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Regression and persistence: remodelling in a tissue engineered axial vascular assembly.

Polykandriotis E, Euler S, Arkudas A, Pryymachuk G, Beier JP, Greil P, Dragu A, Lametschwandtner A, Kneser U, Horch RE - J. Cell. Mol. Med. (2009)

Bottom Line: After the fourth week the absolute number of vessels within the ROI decreased (P < 0.03) whereas, on the contrary, the fractional area of all segments increased (P < 0.02).The variance in calibre was significantly increased in the 8-week group (P < 0.05).Phenomena of remodelling were evaluated quantitatively in a neovascular network and variance could be proposed as a parameter of net vascular maturation.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Hand Surgery, University of Erlangen, Erlangen, Germany. elias.polykandriotis@uk-erlangen.de

ABSTRACT
In later stages of vasculoangiogenesis a vascular network is going through a metamorphosis for optimal perfusion and economy of energy. In this study we make a quantitative approach to phenomena of remodelling in a bioartificial neovascular network and suggest variance of calibre as a parameter of neovascular maturation. For this study, 18 male Lewis rats were subjected to the AV loop operation in combination with a hard porous biogenic matrix and an isolation chamber. The animals were allocated into three groups for different explantation intervals set to 2, 4 and 8 weeks, respectively. Collective attributes like vascular density, percent fractional area and variance of calibre were evaluated for a predefined region of interest (ROI). Late morphogenesis was evaluated by means of scanning electron microscopy. After the fourth week the absolute number of vessels within the ROI decreased (P < 0.03) whereas, on the contrary, the fractional area of all segments increased (P < 0.02). The variance in calibre was significantly increased in the 8-week group (P < 0.05). Lymphatic growth after week 4, early pericyte migration as well as intussusceptive angiogenesis were identified immunohistologically. Phenomena of remodelling were evaluated quantitatively in a neovascular network and variance could be proposed as a parameter of net vascular maturation.

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Luminal diameters (calibre) of individual vessels within a ROI were measured and the variance of calibre was extracted for each ROI. In an attempt to evaluate variance of calibre as a morphologic indicator of maturation for a given neovascular network, this parameter was investigated for all time intervals. The 8-week group presented a significantly higher variance of vascular calibre than either of the 2- and 4-week groups. For each bar n= 5, 30 independent measurements, (*): P < 0.05. Error indicators show standard error of means.
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fig05: Luminal diameters (calibre) of individual vessels within a ROI were measured and the variance of calibre was extracted for each ROI. In an attempt to evaluate variance of calibre as a morphologic indicator of maturation for a given neovascular network, this parameter was investigated for all time intervals. The 8-week group presented a significantly higher variance of vascular calibre than either of the 2- and 4-week groups. For each bar n= 5, 30 independent measurements, (*): P < 0.05. Error indicators show standard error of means.

Mentions: The variance of calibre of vascular lumina detected in the ROI at all segments was as follows: 2 weeks: 215.143 × 103 (SE 88.317 × 103), 4 weeks: 105.619 × 103 (SEM 31.108 × 103), 8 weeks: 643.455 × 103 (SEM 186.243 × 103). The variance in calibre increased significantly between weeks 2 and 8 (P < 0.05) as well as between weeks 4 and 8 (P < 0.01). Change of variance between weeks 2 and 4 was not significant (P= 0.18). The results are summarized in Figs 4 and 5.


Regression and persistence: remodelling in a tissue engineered axial vascular assembly.

Polykandriotis E, Euler S, Arkudas A, Pryymachuk G, Beier JP, Greil P, Dragu A, Lametschwandtner A, Kneser U, Horch RE - J. Cell. Mol. Med. (2009)

Luminal diameters (calibre) of individual vessels within a ROI were measured and the variance of calibre was extracted for each ROI. In an attempt to evaluate variance of calibre as a morphologic indicator of maturation for a given neovascular network, this parameter was investigated for all time intervals. The 8-week group presented a significantly higher variance of vascular calibre than either of the 2- and 4-week groups. For each bar n= 5, 30 independent measurements, (*): P < 0.05. Error indicators show standard error of means.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4496123&req=5

fig05: Luminal diameters (calibre) of individual vessels within a ROI were measured and the variance of calibre was extracted for each ROI. In an attempt to evaluate variance of calibre as a morphologic indicator of maturation for a given neovascular network, this parameter was investigated for all time intervals. The 8-week group presented a significantly higher variance of vascular calibre than either of the 2- and 4-week groups. For each bar n= 5, 30 independent measurements, (*): P < 0.05. Error indicators show standard error of means.
Mentions: The variance of calibre of vascular lumina detected in the ROI at all segments was as follows: 2 weeks: 215.143 × 103 (SE 88.317 × 103), 4 weeks: 105.619 × 103 (SEM 31.108 × 103), 8 weeks: 643.455 × 103 (SEM 186.243 × 103). The variance in calibre increased significantly between weeks 2 and 8 (P < 0.05) as well as between weeks 4 and 8 (P < 0.01). Change of variance between weeks 2 and 4 was not significant (P= 0.18). The results are summarized in Figs 4 and 5.

Bottom Line: After the fourth week the absolute number of vessels within the ROI decreased (P < 0.03) whereas, on the contrary, the fractional area of all segments increased (P < 0.02).The variance in calibre was significantly increased in the 8-week group (P < 0.05).Phenomena of remodelling were evaluated quantitatively in a neovascular network and variance could be proposed as a parameter of net vascular maturation.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Hand Surgery, University of Erlangen, Erlangen, Germany. elias.polykandriotis@uk-erlangen.de

ABSTRACT
In later stages of vasculoangiogenesis a vascular network is going through a metamorphosis for optimal perfusion and economy of energy. In this study we make a quantitative approach to phenomena of remodelling in a bioartificial neovascular network and suggest variance of calibre as a parameter of neovascular maturation. For this study, 18 male Lewis rats were subjected to the AV loop operation in combination with a hard porous biogenic matrix and an isolation chamber. The animals were allocated into three groups for different explantation intervals set to 2, 4 and 8 weeks, respectively. Collective attributes like vascular density, percent fractional area and variance of calibre were evaluated for a predefined region of interest (ROI). Late morphogenesis was evaluated by means of scanning electron microscopy. After the fourth week the absolute number of vessels within the ROI decreased (P < 0.03) whereas, on the contrary, the fractional area of all segments increased (P < 0.02). The variance in calibre was significantly increased in the 8-week group (P < 0.05). Lymphatic growth after week 4, early pericyte migration as well as intussusceptive angiogenesis were identified immunohistologically. Phenomena of remodelling were evaluated quantitatively in a neovascular network and variance could be proposed as a parameter of net vascular maturation.

Show MeSH
Related in: MedlinePlus