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In vitro analysis of integrin expression in stem cells from bone marrow and cord blood during chondrogenic differentiation.

Goessler UR, Bugert P, Bieback K, Stern-Straeter J, Bran G, Sadick H, Hörmann K, Riedel F - J. Cell. Mol. Med. (2008)

Bottom Line: For all integrins, no significant differences could be found in the two types of MSC.Integrin-mediated signalling seems to play an important role in the generation and maintenance of the chondrocytic phenotype during chondrogenic differentiation.To fully harness the potential of these cells, future studies should be directed to ascertain their cellular and molecular characteristics for optimal identification, isolation and expansion.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Head and Neck Surgery, University Hospital Mannheim, University of Heidelberg, Germany. ulrich.goessler@hno.ma.uni-heidelberg.de <ulrich.goessler@hno.ma.uni-heidelberg.de>

ABSTRACT
The use of adult mesenchymal stem cells (MSC) in cartilage tissue engineering has been implemented in the field of regenerative medicine and offers new perspectives in the generation of transplants for reconstructive surgery. The extracellular matrix (ECM) plays a key role in modulating function and phenotype of the embedded cells and contains the integrins as adhesion receptors mediating cell-cell and cell-matrix interactions. In our study, characteristic changes in integrin expression during the course of chondrogenic differentiation of MSC from bone marrow and foetal cord blood were compared. MSC were isolated from bone marrow biopsies and cord blood. During cell culture, chondrogenic differentiation was performed. The expression of integrins and their signalling components were analysed with microarray and immunohistochemistry in freshly isolated MSC and after chondrogenic differentiation. The fibronectin-receptor (integrin a5b1) was expressed by undifferentiated MSC, expression rose during chondrogenic differentiation in both types of MSC. The components of the vitronectin/osteopontin-receptors (avb5) were not expressed by freshly isolated MSC, expression rose with ongoing differentiation. Receptors for collagens (a1b1, a2b1, a3b1) were weakly expressed by undifferentiated MSC and were activated during differentiation. As intracellular signalling components integrin linked kinase (ILK) and CD47 showed increasing expression with ongoing differentiation. For all integrins, no significant differences could be found in the two types of MSC. Integrin-mediated signalling seems to play an important role in the generation and maintenance of the chondrocytic phenotype during chondrogenic differentiation. Especially the receptors for fibronectin, vitronectin, osteopontin and collagens might be involved in the generation of the ECM. Intracellularly, their signals might be transduced by ILK and CD47. To fully harness the potential of these cells, future studies should be directed to ascertain their cellular and molecular characteristics for optimal identification, isolation and expansion.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical staining against different integrins in MSC during chondrogenic differentiation. Day 1 (left) and day 20 (right) of cell culture. A Integrin β1 (day 1) in CB MSC; B Integrin β1 (day 20) in CB MSC; C ILK (day 1) in CB MSC; D ILK (day 20) in CB MSC.
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fig03: Immunohistochemical staining against different integrins in MSC during chondrogenic differentiation. Day 1 (left) and day 20 (right) of cell culture. A Integrin β1 (day 1) in CB MSC; B Integrin β1 (day 20) in CB MSC; C ILK (day 1) in CB MSC; D ILK (day 20) in CB MSC.

Mentions: The analysis of integrin-expression on protein-level was analysed with monoclonal antibodys against integrin av, integrin β1, integrin β5, CD47 and the integrin-linked kinase (ILK). For all markers, during the whole process of chondrogenic differentiation, a constant expression for all markers could be found (Fig. 3, Table 4).


In vitro analysis of integrin expression in stem cells from bone marrow and cord blood during chondrogenic differentiation.

Goessler UR, Bugert P, Bieback K, Stern-Straeter J, Bran G, Sadick H, Hörmann K, Riedel F - J. Cell. Mol. Med. (2008)

Immunohistochemical staining against different integrins in MSC during chondrogenic differentiation. Day 1 (left) and day 20 (right) of cell culture. A Integrin β1 (day 1) in CB MSC; B Integrin β1 (day 20) in CB MSC; C ILK (day 1) in CB MSC; D ILK (day 20) in CB MSC.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4496112&req=5

fig03: Immunohistochemical staining against different integrins in MSC during chondrogenic differentiation. Day 1 (left) and day 20 (right) of cell culture. A Integrin β1 (day 1) in CB MSC; B Integrin β1 (day 20) in CB MSC; C ILK (day 1) in CB MSC; D ILK (day 20) in CB MSC.
Mentions: The analysis of integrin-expression on protein-level was analysed with monoclonal antibodys against integrin av, integrin β1, integrin β5, CD47 and the integrin-linked kinase (ILK). For all markers, during the whole process of chondrogenic differentiation, a constant expression for all markers could be found (Fig. 3, Table 4).

Bottom Line: For all integrins, no significant differences could be found in the two types of MSC.Integrin-mediated signalling seems to play an important role in the generation and maintenance of the chondrocytic phenotype during chondrogenic differentiation.To fully harness the potential of these cells, future studies should be directed to ascertain their cellular and molecular characteristics for optimal identification, isolation and expansion.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Head and Neck Surgery, University Hospital Mannheim, University of Heidelberg, Germany. ulrich.goessler@hno.ma.uni-heidelberg.de <ulrich.goessler@hno.ma.uni-heidelberg.de>

ABSTRACT
The use of adult mesenchymal stem cells (MSC) in cartilage tissue engineering has been implemented in the field of regenerative medicine and offers new perspectives in the generation of transplants for reconstructive surgery. The extracellular matrix (ECM) plays a key role in modulating function and phenotype of the embedded cells and contains the integrins as adhesion receptors mediating cell-cell and cell-matrix interactions. In our study, characteristic changes in integrin expression during the course of chondrogenic differentiation of MSC from bone marrow and foetal cord blood were compared. MSC were isolated from bone marrow biopsies and cord blood. During cell culture, chondrogenic differentiation was performed. The expression of integrins and their signalling components were analysed with microarray and immunohistochemistry in freshly isolated MSC and after chondrogenic differentiation. The fibronectin-receptor (integrin a5b1) was expressed by undifferentiated MSC, expression rose during chondrogenic differentiation in both types of MSC. The components of the vitronectin/osteopontin-receptors (avb5) were not expressed by freshly isolated MSC, expression rose with ongoing differentiation. Receptors for collagens (a1b1, a2b1, a3b1) were weakly expressed by undifferentiated MSC and were activated during differentiation. As intracellular signalling components integrin linked kinase (ILK) and CD47 showed increasing expression with ongoing differentiation. For all integrins, no significant differences could be found in the two types of MSC. Integrin-mediated signalling seems to play an important role in the generation and maintenance of the chondrocytic phenotype during chondrogenic differentiation. Especially the receptors for fibronectin, vitronectin, osteopontin and collagens might be involved in the generation of the ECM. Intracellularly, their signals might be transduced by ILK and CD47. To fully harness the potential of these cells, future studies should be directed to ascertain their cellular and molecular characteristics for optimal identification, isolation and expansion.

No MeSH data available.


Related in: MedlinePlus