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Carryover Effects of Acute DEHP Exposure on Ovarian Function and Oocyte Developmental Competence in Lactating Cows.

Kalo D, Hadas R, Furman O, Ben-Ari J, Maor Y, Patterson DG, Tomey C, Roth Z - PLoS ONE (2015)

Bottom Line: Findings revealed that DEHP impairs the pattern of follicular development, with a prominent effect on dominant follicles.Estradiol concentration in the follicular fluid was lower in the DEHP-treated group than in controls, and associated with a higher number of follicular pathologies (follicle diameter >25 mm).The results describe the risk associated with acute exposure to DEHP and its deleterious carryover effects on ovarian function, nuclear maturation and oocyte developmental competence.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, the Hebrew University, Rehovot, 76100, Israel; Center of Excellence in Agriculture and Environmental Health, Robert H. Smith Faculty of Agriculture, Food and Environment, the Hebrew University, Rehovot, 76100, Israel.

ABSTRACT
We examined acute exposure of Holstein cows to di(2-ethylhexyl) phthalate (DEHP) and its carryover effects on ovarian function and oocyte developmental competence. Synchronized cows were tube-fed with water or 100 mg/kg DEHP per day for 3 days. Blood, urine and milk samples were collected before, during and after DEHP exposure to examine its clearance pattern. Ovarian follicular dynamics was monitored through an entire estrous cycle by ultrasonographic scanning. Follicular fluids were aspirated from the preovulatory follicles on days 0 and 29 of the experiment and analyzed for phthalate metabolites and estradiol concentration. The aspirated follicular fluid was used as maturation medium for in-vitro embryo production. Findings revealed that DEHP impairs the pattern of follicular development, with a prominent effect on dominant follicles. The diameter and growth rate of the first- and second-wave dominant follicles were lower (P < 0.05) in the DEHP-treated group. Estradiol concentration in the follicular fluid was lower in the DEHP-treated group than in controls, and associated with a higher number of follicular pathologies (follicle diameter >25 mm). The pattern of growth and regression of the corpus luteum differed between groups, with a lower volume in the DEHP-treated group (P < 0.05). The follicular fluid aspirated from the DEHP-treated group, but not the controls, contained 23 nM mono(2-ethylhexyl) phthalate. Culturing of cumulus oocyte complexes in the follicular fluid aspirated from DEHP-treated cows reduced the proportion of oocytes progressing to the MII stage, and the proportions of 2- to 4-cell-stage embryos (P < 0.04) and 7-day blastocysts (P < 0.06). The results describe the risk associated with acute exposure to DEHP and its deleterious carryover effects on ovarian function, nuclear maturation and oocyte developmental competence.

No MeSH data available.


Related in: MedlinePlus

DEHP-metabolite concentrations in the plasma before, during and after DEHP exposure.Cows were administered with water (control) or 100 mg/kg DEHP per day on days 1–3 of the experiment (DEHP-treated). Blood samples were taken before (day 0), during (days 2 and 4) and after (days 11, 19 and 24) DEHP exposure. Samples were analyzed for concentrations of DEHP metabolites mono(2-ethylhexyl) phthalate (MEHP), mono(2-ethyl-5-hydroxyhexyl) phthalate (5OH-MEHP), mono(2-ethyl-5-oxohexyl) phthalate (5oxo-MEHP), mono(2-ethyl-5-carboxypentyl) phthalate (5cx-MEPP) and mono[2-(carboxymethyl)hexyl] phthalate (2cx-MMHP) by LC–MS/MS.
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pone.0130896.g002: DEHP-metabolite concentrations in the plasma before, during and after DEHP exposure.Cows were administered with water (control) or 100 mg/kg DEHP per day on days 1–3 of the experiment (DEHP-treated). Blood samples were taken before (day 0), during (days 2 and 4) and after (days 11, 19 and 24) DEHP exposure. Samples were analyzed for concentrations of DEHP metabolites mono(2-ethylhexyl) phthalate (MEHP), mono(2-ethyl-5-hydroxyhexyl) phthalate (5OH-MEHP), mono(2-ethyl-5-oxohexyl) phthalate (5oxo-MEHP), mono(2-ethyl-5-carboxypentyl) phthalate (5cx-MEPP) and mono[2-(carboxymethyl)hexyl] phthalate (2cx-MMHP) by LC–MS/MS.

Mentions: On day 0 of the experiment (i.e., before DEHP exposure), the average levels of all examined DEHP metabolites were relatively low in all three fractions (plasma, milk and urine). In the control group, DEHP-metabolite levels remained low in all collected fractions throughout the experiment and did not differ from levels detected on day 0 (Table 2). In the DEHP-treated cows, DEHP-metabolite levels increased (P < 0.05) in all examined fractions during DEHP exposure (days 2 and 4), followed by rapid clearance throughout the subsequent days of the experiment (days 11, 19 and 24) to levels similar to those detected in the control group (Table 2). For instance, the highest MEHP level in the plasma reached up to 56.58 μM during DEHP exposure and decreased to 5.74 nM over subsequent days to a level similar to those detected before DEHP exposure (12.21 nM) and in the control group (15.5 ± 2.9 nM; Fig 2).


Carryover Effects of Acute DEHP Exposure on Ovarian Function and Oocyte Developmental Competence in Lactating Cows.

Kalo D, Hadas R, Furman O, Ben-Ari J, Maor Y, Patterson DG, Tomey C, Roth Z - PLoS ONE (2015)

DEHP-metabolite concentrations in the plasma before, during and after DEHP exposure.Cows were administered with water (control) or 100 mg/kg DEHP per day on days 1–3 of the experiment (DEHP-treated). Blood samples were taken before (day 0), during (days 2 and 4) and after (days 11, 19 and 24) DEHP exposure. Samples were analyzed for concentrations of DEHP metabolites mono(2-ethylhexyl) phthalate (MEHP), mono(2-ethyl-5-hydroxyhexyl) phthalate (5OH-MEHP), mono(2-ethyl-5-oxohexyl) phthalate (5oxo-MEHP), mono(2-ethyl-5-carboxypentyl) phthalate (5cx-MEPP) and mono[2-(carboxymethyl)hexyl] phthalate (2cx-MMHP) by LC–MS/MS.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496077&req=5

pone.0130896.g002: DEHP-metabolite concentrations in the plasma before, during and after DEHP exposure.Cows were administered with water (control) or 100 mg/kg DEHP per day on days 1–3 of the experiment (DEHP-treated). Blood samples were taken before (day 0), during (days 2 and 4) and after (days 11, 19 and 24) DEHP exposure. Samples were analyzed for concentrations of DEHP metabolites mono(2-ethylhexyl) phthalate (MEHP), mono(2-ethyl-5-hydroxyhexyl) phthalate (5OH-MEHP), mono(2-ethyl-5-oxohexyl) phthalate (5oxo-MEHP), mono(2-ethyl-5-carboxypentyl) phthalate (5cx-MEPP) and mono[2-(carboxymethyl)hexyl] phthalate (2cx-MMHP) by LC–MS/MS.
Mentions: On day 0 of the experiment (i.e., before DEHP exposure), the average levels of all examined DEHP metabolites were relatively low in all three fractions (plasma, milk and urine). In the control group, DEHP-metabolite levels remained low in all collected fractions throughout the experiment and did not differ from levels detected on day 0 (Table 2). In the DEHP-treated cows, DEHP-metabolite levels increased (P < 0.05) in all examined fractions during DEHP exposure (days 2 and 4), followed by rapid clearance throughout the subsequent days of the experiment (days 11, 19 and 24) to levels similar to those detected in the control group (Table 2). For instance, the highest MEHP level in the plasma reached up to 56.58 μM during DEHP exposure and decreased to 5.74 nM over subsequent days to a level similar to those detected before DEHP exposure (12.21 nM) and in the control group (15.5 ± 2.9 nM; Fig 2).

Bottom Line: Findings revealed that DEHP impairs the pattern of follicular development, with a prominent effect on dominant follicles.Estradiol concentration in the follicular fluid was lower in the DEHP-treated group than in controls, and associated with a higher number of follicular pathologies (follicle diameter >25 mm).The results describe the risk associated with acute exposure to DEHP and its deleterious carryover effects on ovarian function, nuclear maturation and oocyte developmental competence.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, the Hebrew University, Rehovot, 76100, Israel; Center of Excellence in Agriculture and Environmental Health, Robert H. Smith Faculty of Agriculture, Food and Environment, the Hebrew University, Rehovot, 76100, Israel.

ABSTRACT
We examined acute exposure of Holstein cows to di(2-ethylhexyl) phthalate (DEHP) and its carryover effects on ovarian function and oocyte developmental competence. Synchronized cows were tube-fed with water or 100 mg/kg DEHP per day for 3 days. Blood, urine and milk samples were collected before, during and after DEHP exposure to examine its clearance pattern. Ovarian follicular dynamics was monitored through an entire estrous cycle by ultrasonographic scanning. Follicular fluids were aspirated from the preovulatory follicles on days 0 and 29 of the experiment and analyzed for phthalate metabolites and estradiol concentration. The aspirated follicular fluid was used as maturation medium for in-vitro embryo production. Findings revealed that DEHP impairs the pattern of follicular development, with a prominent effect on dominant follicles. The diameter and growth rate of the first- and second-wave dominant follicles were lower (P < 0.05) in the DEHP-treated group. Estradiol concentration in the follicular fluid was lower in the DEHP-treated group than in controls, and associated with a higher number of follicular pathologies (follicle diameter >25 mm). The pattern of growth and regression of the corpus luteum differed between groups, with a lower volume in the DEHP-treated group (P < 0.05). The follicular fluid aspirated from the DEHP-treated group, but not the controls, contained 23 nM mono(2-ethylhexyl) phthalate. Culturing of cumulus oocyte complexes in the follicular fluid aspirated from DEHP-treated cows reduced the proportion of oocytes progressing to the MII stage, and the proportions of 2- to 4-cell-stage embryos (P < 0.04) and 7-day blastocysts (P < 0.06). The results describe the risk associated with acute exposure to DEHP and its deleterious carryover effects on ovarian function, nuclear maturation and oocyte developmental competence.

No MeSH data available.


Related in: MedlinePlus