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Maternal Plane of Nutrition during Late Gestation and Weaning Age Alter Angus × Simmental Offspring Longissimus Muscle Transcriptome and Intramuscular Fat.

Moisá SJ, Shike DW, Shoup L, Rodriguez-Zas SL, Loor JJ - PLoS ONE (2015)

Bottom Line: Carcass intramuscular fat content was greater in EW offspring.Bioinformatics analysis of the transcriptome highlighted a modest overall response to maternal plane of nutrition, resulting in only 35 differentially expressed genes (DEG).Additional knowledge on molecular and functional outcomes would help produce more efficient beef cattle.

View Article: PubMed Central - PubMed

Affiliation: Mammalian NutriPhysioGenomics, Department of Animal Sciences, University of Illinois, Urbana, Illinois, United States of America.

ABSTRACT
In model organisms both the nutrition of the mother and the young offspring could induce long-lasting transcriptional changes in tissues. In livestock, such changes could have important roles in determining nutrient use and meat quality. The main objective was to evaluate if plane of maternal nutrition during late-gestation and weaning age alter the offspring's Longissimus muscle (LM) transcriptome, animal performance, and metabolic hormones. Whole-transcriptome microarray analysis was performed on LM samples of early (EW) and normal weaned (NW) Angus × Simmental calves born to grazing cows receiving no supplement [low plane of nutrition (LPN)] or 2.3 kg high-grain mix/day [medium plane of nutrition (MPN)] during the last 105 days of gestation. Biopsies of LM were harvested at 78 (EW), 187 (NW) and 354 (before slaughter) days of age. Despite greater feed intake in MPN offspring, blood insulin was greater in LPN offspring. Carcass intramuscular fat content was greater in EW offspring. Bioinformatics analysis of the transcriptome highlighted a modest overall response to maternal plane of nutrition, resulting in only 35 differentially expressed genes (DEG). However, weaning age and a high-grain diet (EW) strongly impacted the transcriptome (DEG = 167), especially causing a lipogenic program activation. In addition, between 78 and 187 days of age, EW steers had an activation of the innate immune system due presumably to macrophage infiltration of intramuscular fat. Between 187 and 354 days of age (the "finishing" phase), NW steers had an activation of the lipogenic transcriptome machinery, while EW steers had a clear inhibition through the epigenetic control of histone acetylases. Results underscored the need to conduct further studies to understand better the functional outcome of transcriptome changes induced in the offspring by pre- and post-natal nutrition. Additional knowledge on molecular and functional outcomes would help produce more efficient beef cattle.

No MeSH data available.


Related in: MedlinePlus

Results of the 3 most impacted pathways within differentially expressed genes affected during the growing phase (187 vs. 78 d), finishing phase (354 vs. 187 d) and the whole experiment (354 vs. 78 d).Analysis performed using the Dynamic Impact Approach (DIA) based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathways database. Flux represents the direction of each category and the corresponding subcategory (green color = inhibition, yellow color = stable, red color = activation with different color intensities according with the level of up-regulation or down-regulation). Blue bars denote the impact of each biological process.
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pone.0131478.g004: Results of the 3 most impacted pathways within differentially expressed genes affected during the growing phase (187 vs. 78 d), finishing phase (354 vs. 187 d) and the whole experiment (354 vs. 78 d).Analysis performed using the Dynamic Impact Approach (DIA) based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathways database. Flux represents the direction of each category and the corresponding subcategory (green color = inhibition, yellow color = stable, red color = activation with different color intensities according with the level of up-regulation or down-regulation). Blue bars denote the impact of each biological process.

Mentions: The DIA analysis was performed with DEG (n = 167) at an uncorrected P value = 0.05 and an FDR = 0.10. Fig 3 contains the summary of KEGG pathways results from DIA. The top-three most impacted canonical pathways are reported in Fig 4 and the genes with highest activation in Fig 5. For biological processes, only those with an impact value higher than 50% of the maximum total impact value for each time comparison for EW and NW steers are discussed (Fig 6).


Maternal Plane of Nutrition during Late Gestation and Weaning Age Alter Angus × Simmental Offspring Longissimus Muscle Transcriptome and Intramuscular Fat.

Moisá SJ, Shike DW, Shoup L, Rodriguez-Zas SL, Loor JJ - PLoS ONE (2015)

Results of the 3 most impacted pathways within differentially expressed genes affected during the growing phase (187 vs. 78 d), finishing phase (354 vs. 187 d) and the whole experiment (354 vs. 78 d).Analysis performed using the Dynamic Impact Approach (DIA) based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathways database. Flux represents the direction of each category and the corresponding subcategory (green color = inhibition, yellow color = stable, red color = activation with different color intensities according with the level of up-regulation or down-regulation). Blue bars denote the impact of each biological process.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4496061&req=5

pone.0131478.g004: Results of the 3 most impacted pathways within differentially expressed genes affected during the growing phase (187 vs. 78 d), finishing phase (354 vs. 187 d) and the whole experiment (354 vs. 78 d).Analysis performed using the Dynamic Impact Approach (DIA) based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathways database. Flux represents the direction of each category and the corresponding subcategory (green color = inhibition, yellow color = stable, red color = activation with different color intensities according with the level of up-regulation or down-regulation). Blue bars denote the impact of each biological process.
Mentions: The DIA analysis was performed with DEG (n = 167) at an uncorrected P value = 0.05 and an FDR = 0.10. Fig 3 contains the summary of KEGG pathways results from DIA. The top-three most impacted canonical pathways are reported in Fig 4 and the genes with highest activation in Fig 5. For biological processes, only those with an impact value higher than 50% of the maximum total impact value for each time comparison for EW and NW steers are discussed (Fig 6).

Bottom Line: Carcass intramuscular fat content was greater in EW offspring.Bioinformatics analysis of the transcriptome highlighted a modest overall response to maternal plane of nutrition, resulting in only 35 differentially expressed genes (DEG).Additional knowledge on molecular and functional outcomes would help produce more efficient beef cattle.

View Article: PubMed Central - PubMed

Affiliation: Mammalian NutriPhysioGenomics, Department of Animal Sciences, University of Illinois, Urbana, Illinois, United States of America.

ABSTRACT
In model organisms both the nutrition of the mother and the young offspring could induce long-lasting transcriptional changes in tissues. In livestock, such changes could have important roles in determining nutrient use and meat quality. The main objective was to evaluate if plane of maternal nutrition during late-gestation and weaning age alter the offspring's Longissimus muscle (LM) transcriptome, animal performance, and metabolic hormones. Whole-transcriptome microarray analysis was performed on LM samples of early (EW) and normal weaned (NW) Angus × Simmental calves born to grazing cows receiving no supplement [low plane of nutrition (LPN)] or 2.3 kg high-grain mix/day [medium plane of nutrition (MPN)] during the last 105 days of gestation. Biopsies of LM were harvested at 78 (EW), 187 (NW) and 354 (before slaughter) days of age. Despite greater feed intake in MPN offspring, blood insulin was greater in LPN offspring. Carcass intramuscular fat content was greater in EW offspring. Bioinformatics analysis of the transcriptome highlighted a modest overall response to maternal plane of nutrition, resulting in only 35 differentially expressed genes (DEG). However, weaning age and a high-grain diet (EW) strongly impacted the transcriptome (DEG = 167), especially causing a lipogenic program activation. In addition, between 78 and 187 days of age, EW steers had an activation of the innate immune system due presumably to macrophage infiltration of intramuscular fat. Between 187 and 354 days of age (the "finishing" phase), NW steers had an activation of the lipogenic transcriptome machinery, while EW steers had a clear inhibition through the epigenetic control of histone acetylases. Results underscored the need to conduct further studies to understand better the functional outcome of transcriptome changes induced in the offspring by pre- and post-natal nutrition. Additional knowledge on molecular and functional outcomes would help produce more efficient beef cattle.

No MeSH data available.


Related in: MedlinePlus