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Survival Rate and Transcriptional Response upon Infection with the Generalist Parasite Beauveria bassiana in a World-Wide Sample of Drosophila melanogaster.

Paparazzo F, Tellier A, Stephan W, Hutter S - PLoS ONE (2015)

Bottom Line: It is thought to be higher in tropical regions compared to temporal ones.We furthermore found that the American population was transcriptionally highly diverged from all other populations concerning basal levels of gene expression.This was particularly true for stress and immune response genes, which might be the genetic basis for their elevated vigor.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology II, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany.

ABSTRACT
The ability to cope with infection by a parasite is one of the major challenges for any host species and is a major driver of evolution. Parasite pressure differs between habitats. It is thought to be higher in tropical regions compared to temporal ones. We infected Drosophila melanogaster from two tropical (Malaysia and Zimbabwe) and two temperate populations (the Netherlands and North Carolina) with the generalist entomopathogenic fungus Beauveria bassiana to examine if adaptation to local parasite pressures led to differences in resistance. Contrary to previous findings we observed increased survival in temperate populations. This, however, is not due to increased resistance to infection per se, but rather the consequence of a higher general vigor of the temperate populations. We also assessed transcriptional response to infection within these flies eight and 24 hours after infection. Only few genes were induced at the earlier time point, most of which are involved in detoxification. In contrast, we identified more than 4,000 genes that changed their expression state after 24 hours. This response was generally conserved over all populations with only few genes being uniquely regulated in the temperate populations. We furthermore found that the American population was transcriptionally highly diverged from all other populations concerning basal levels of gene expression. This was particularly true for stress and immune response genes, which might be the genetic basis for their elevated vigor.

No MeSH data available.


Related in: MedlinePlus

Comparison of strongly induced genes.Overlap of the 100 most strongly induced genes 24 hours after infection in this study compared to those found in De Gregorio et al. [19] and Roxström-Lundquist et al. [20]. Gene names of the genes found in the microarray studies were updated to reflect the genome annotation of Flybase release 5.57 [36].
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pone.0132129.g003: Comparison of strongly induced genes.Overlap of the 100 most strongly induced genes 24 hours after infection in this study compared to those found in De Gregorio et al. [19] and Roxström-Lundquist et al. [20]. Gene names of the genes found in the microarray studies were updated to reflect the genome annotation of Flybase release 5.57 [36].

Mentions: We then compared our list of strongly induced genes with those found in previous microarray studies at the same time point (Fig 3). We find that several genes overlap between the studies, especially those belonging to the immune induced molecule gene family. However, overall the proportion of overlapping genes is rather small. Also, several genes that were found to be strongly induced in both microarray studies (e.g. Drosomycin, Metchnikowin) were not found to be induced in our experiments. The overlap is even smaller when looking at genes that are strongly repressed. Here we found that only a single gene, the odorant-binding protein Obp99b, was shared between our study and the work of Röxstrom-Lundquist et al. [20], while the genes lectin-21Cb and CG18179 overlapped with the data set of De Gregorio et al. [19].


Survival Rate and Transcriptional Response upon Infection with the Generalist Parasite Beauveria bassiana in a World-Wide Sample of Drosophila melanogaster.

Paparazzo F, Tellier A, Stephan W, Hutter S - PLoS ONE (2015)

Comparison of strongly induced genes.Overlap of the 100 most strongly induced genes 24 hours after infection in this study compared to those found in De Gregorio et al. [19] and Roxström-Lundquist et al. [20]. Gene names of the genes found in the microarray studies were updated to reflect the genome annotation of Flybase release 5.57 [36].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4495925&req=5

pone.0132129.g003: Comparison of strongly induced genes.Overlap of the 100 most strongly induced genes 24 hours after infection in this study compared to those found in De Gregorio et al. [19] and Roxström-Lundquist et al. [20]. Gene names of the genes found in the microarray studies were updated to reflect the genome annotation of Flybase release 5.57 [36].
Mentions: We then compared our list of strongly induced genes with those found in previous microarray studies at the same time point (Fig 3). We find that several genes overlap between the studies, especially those belonging to the immune induced molecule gene family. However, overall the proportion of overlapping genes is rather small. Also, several genes that were found to be strongly induced in both microarray studies (e.g. Drosomycin, Metchnikowin) were not found to be induced in our experiments. The overlap is even smaller when looking at genes that are strongly repressed. Here we found that only a single gene, the odorant-binding protein Obp99b, was shared between our study and the work of Röxstrom-Lundquist et al. [20], while the genes lectin-21Cb and CG18179 overlapped with the data set of De Gregorio et al. [19].

Bottom Line: It is thought to be higher in tropical regions compared to temporal ones.We furthermore found that the American population was transcriptionally highly diverged from all other populations concerning basal levels of gene expression.This was particularly true for stress and immune response genes, which might be the genetic basis for their elevated vigor.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology II, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany.

ABSTRACT
The ability to cope with infection by a parasite is one of the major challenges for any host species and is a major driver of evolution. Parasite pressure differs between habitats. It is thought to be higher in tropical regions compared to temporal ones. We infected Drosophila melanogaster from two tropical (Malaysia and Zimbabwe) and two temperate populations (the Netherlands and North Carolina) with the generalist entomopathogenic fungus Beauveria bassiana to examine if adaptation to local parasite pressures led to differences in resistance. Contrary to previous findings we observed increased survival in temperate populations. This, however, is not due to increased resistance to infection per se, but rather the consequence of a higher general vigor of the temperate populations. We also assessed transcriptional response to infection within these flies eight and 24 hours after infection. Only few genes were induced at the earlier time point, most of which are involved in detoxification. In contrast, we identified more than 4,000 genes that changed their expression state after 24 hours. This response was generally conserved over all populations with only few genes being uniquely regulated in the temperate populations. We furthermore found that the American population was transcriptionally highly diverged from all other populations concerning basal levels of gene expression. This was particularly true for stress and immune response genes, which might be the genetic basis for their elevated vigor.

No MeSH data available.


Related in: MedlinePlus