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Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells.

Aziz J, Abu Kassim NL, Abu Kasim NH, Haque N, Rahman MT - BMC Complement Altern Med (2015)

Bottom Line: Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papaya pulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF) RESULTS: In vitro scratch gap closure was significantly faster (p < .05) in SHED culture treated with UPJ.IL-6 concentration was significantly increased (p < .05) in SHED and PBL culture supernatant when treated with UPJ.SCF synthesis in SHED culture was also significantly increased (p < .05) when treated with UPJ CONCLUSION: In vitro upregulated synthesis of IL -6 and SCF both in PBL and SHED reveals the potential mechanism of unripe papaya to induce thrombopoietic cytokines synthesis in cells of hematopoietic and mesenchymal origin.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, International Islamic University Malaysia, Bandar Indera Mahkota, Kuantan, 25200, Malaysia. jazliaziz87@gmail.com.

ABSTRACT

Background: Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction

Methods: In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papaya pulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF) RESULTS: In vitro scratch gap closure was significantly faster (p < .05) in SHED culture treated with UPJ. IL-6 concentration was significantly increased (p < .05) in SHED and PBL culture supernatant when treated with UPJ. SCF synthesis in SHED culture was also significantly increased (p < .05) when treated with UPJ CONCLUSION: In vitro upregulated synthesis of IL -6 and SCF both in PBL and SHED reveals the potential mechanism of unripe papaya to induce thrombopoietic cytokines synthesis in cells of hematopoietic and mesenchymal origin.

No MeSH data available.


Related in: MedlinePlus

Proposed mechanism of IL-6 induced thrombocyte production. Addition of UPJ may enhance IL-6 secretion by both haematopoietic stem cells (HSC) and mesenchymal stem cells (MSC) such as SHED. IL-6 can then directly enhance HSC proliferation, maturation and differentiation, or act on the liver to stimulate higher production of TPO, the chief cytokine involved in megakaryopoiesis and thrombopoiesis. Increased synthesis of SCF by MSC may also act on TPO to further stimulate megakaryopoiesis and thrombopoiesis. [CMP: common myeloid progenitor; MK: megakaryocyte.]
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Fig4: Proposed mechanism of IL-6 induced thrombocyte production. Addition of UPJ may enhance IL-6 secretion by both haematopoietic stem cells (HSC) and mesenchymal stem cells (MSC) such as SHED. IL-6 can then directly enhance HSC proliferation, maturation and differentiation, or act on the liver to stimulate higher production of TPO, the chief cytokine involved in megakaryopoiesis and thrombopoiesis. Increased synthesis of SCF by MSC may also act on TPO to further stimulate megakaryopoiesis and thrombopoiesis. [CMP: common myeloid progenitor; MK: megakaryocyte.]

Mentions: Since TPO is the major cytokine involved in megakaryopoiesis and thrombopoiesis, it is possible that papaya extract/juice enhances thrombocyte production by first increasing IL-6 expression in stem cells and leukocytes, as shown in this study, which in turn enhances the production of TPO in the liver, leading to an increased rate of thrombocyte production (Fig. 4).Fig. 4


Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells.

Aziz J, Abu Kassim NL, Abu Kasim NH, Haque N, Rahman MT - BMC Complement Altern Med (2015)

Proposed mechanism of IL-6 induced thrombocyte production. Addition of UPJ may enhance IL-6 secretion by both haematopoietic stem cells (HSC) and mesenchymal stem cells (MSC) such as SHED. IL-6 can then directly enhance HSC proliferation, maturation and differentiation, or act on the liver to stimulate higher production of TPO, the chief cytokine involved in megakaryopoiesis and thrombopoiesis. Increased synthesis of SCF by MSC may also act on TPO to further stimulate megakaryopoiesis and thrombopoiesis. [CMP: common myeloid progenitor; MK: megakaryocyte.]
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4495848&req=5

Fig4: Proposed mechanism of IL-6 induced thrombocyte production. Addition of UPJ may enhance IL-6 secretion by both haematopoietic stem cells (HSC) and mesenchymal stem cells (MSC) such as SHED. IL-6 can then directly enhance HSC proliferation, maturation and differentiation, or act on the liver to stimulate higher production of TPO, the chief cytokine involved in megakaryopoiesis and thrombopoiesis. Increased synthesis of SCF by MSC may also act on TPO to further stimulate megakaryopoiesis and thrombopoiesis. [CMP: common myeloid progenitor; MK: megakaryocyte.]
Mentions: Since TPO is the major cytokine involved in megakaryopoiesis and thrombopoiesis, it is possible that papaya extract/juice enhances thrombocyte production by first increasing IL-6 expression in stem cells and leukocytes, as shown in this study, which in turn enhances the production of TPO in the liver, leading to an increased rate of thrombocyte production (Fig. 4).Fig. 4

Bottom Line: Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papaya pulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF) RESULTS: In vitro scratch gap closure was significantly faster (p < .05) in SHED culture treated with UPJ.IL-6 concentration was significantly increased (p < .05) in SHED and PBL culture supernatant when treated with UPJ.SCF synthesis in SHED culture was also significantly increased (p < .05) when treated with UPJ CONCLUSION: In vitro upregulated synthesis of IL -6 and SCF both in PBL and SHED reveals the potential mechanism of unripe papaya to induce thrombopoietic cytokines synthesis in cells of hematopoietic and mesenchymal origin.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, International Islamic University Malaysia, Bandar Indera Mahkota, Kuantan, 25200, Malaysia. jazliaziz87@gmail.com.

ABSTRACT

Background: Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction

Methods: In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papaya pulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF) RESULTS: In vitro scratch gap closure was significantly faster (p < .05) in SHED culture treated with UPJ. IL-6 concentration was significantly increased (p < .05) in SHED and PBL culture supernatant when treated with UPJ. SCF synthesis in SHED culture was also significantly increased (p < .05) when treated with UPJ CONCLUSION: In vitro upregulated synthesis of IL -6 and SCF both in PBL and SHED reveals the potential mechanism of unripe papaya to induce thrombopoietic cytokines synthesis in cells of hematopoietic and mesenchymal origin.

No MeSH data available.


Related in: MedlinePlus