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Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells.

Aziz J, Abu Kassim NL, Abu Kasim NH, Haque N, Rahman MT - BMC Complement Altern Med (2015)

Bottom Line: Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papaya pulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF) RESULTS: In vitro scratch gap closure was significantly faster (p < .05) in SHED culture treated with UPJ.IL-6 concentration was significantly increased (p < .05) in SHED and PBL culture supernatant when treated with UPJ.SCF synthesis in SHED culture was also significantly increased (p < .05) when treated with UPJ CONCLUSION: In vitro upregulated synthesis of IL -6 and SCF both in PBL and SHED reveals the potential mechanism of unripe papaya to induce thrombopoietic cytokines synthesis in cells of hematopoietic and mesenchymal origin.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, International Islamic University Malaysia, Bandar Indera Mahkota, Kuantan, 25200, Malaysia. jazliaziz87@gmail.com.

ABSTRACT

Background: Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction

Methods: In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papaya pulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF) RESULTS: In vitro scratch gap closure was significantly faster (p < .05) in SHED culture treated with UPJ. IL-6 concentration was significantly increased (p < .05) in SHED and PBL culture supernatant when treated with UPJ. SCF synthesis in SHED culture was also significantly increased (p < .05) when treated with UPJ CONCLUSION: In vitro upregulated synthesis of IL -6 and SCF both in PBL and SHED reveals the potential mechanism of unripe papaya to induce thrombopoietic cytokines synthesis in cells of hematopoietic and mesenchymal origin.

No MeSH data available.


Related in: MedlinePlus

Unripe papaya pulp juice enhances in vitro IL-6 synthesis in SHED in dose dependent manner. A significant increase in IL-6 (a) synthesis was found in the 10% UPJ group compared to the control, 2% UPJ and 5% UPJ groups (p = .008), while the 5% UPJ group produced significantly higher IL-6 compared to control and the 2% UPJ group (p = .008). Synthesis of IL-3 (b), TPO (c) and SCF (d) was not significantly different between groups. *Significantly higher compared to control and 2% UPJ group (p = .008); #Significantly higher than 5% UPJ group (p = .008); n = 9
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Fig2: Unripe papaya pulp juice enhances in vitro IL-6 synthesis in SHED in dose dependent manner. A significant increase in IL-6 (a) synthesis was found in the 10% UPJ group compared to the control, 2% UPJ and 5% UPJ groups (p = .008), while the 5% UPJ group produced significantly higher IL-6 compared to control and the 2% UPJ group (p = .008). Synthesis of IL-3 (b), TPO (c) and SCF (d) was not significantly different between groups. *Significantly higher compared to control and 2% UPJ group (p = .008); #Significantly higher than 5% UPJ group (p = .008); n = 9

Mentions: Of the six cytokines measured, only IL-6, IL-3, TPO and SCF were detectable in the assay (Fig. 2), while G-CSF and GM-CSF were below the assay’s level of detection. IL-6 was significantly increased (p = .008) with the addition of 5% and 10% UPJ (Fig. 2a). increase of SCF secretion compared to the control after addition of 5% and 10% UPJ was statistically significant (p = .046 and p = .018 respectively) (Fig. 2d). Concentration of IL-3 declined with the addition of higher concentrations of UPJ (Fig. 2b) while concentration of TPO remained relatively constant across all groups (Fig. 2c).Fig. 2


Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells.

Aziz J, Abu Kassim NL, Abu Kasim NH, Haque N, Rahman MT - BMC Complement Altern Med (2015)

Unripe papaya pulp juice enhances in vitro IL-6 synthesis in SHED in dose dependent manner. A significant increase in IL-6 (a) synthesis was found in the 10% UPJ group compared to the control, 2% UPJ and 5% UPJ groups (p = .008), while the 5% UPJ group produced significantly higher IL-6 compared to control and the 2% UPJ group (p = .008). Synthesis of IL-3 (b), TPO (c) and SCF (d) was not significantly different between groups. *Significantly higher compared to control and 2% UPJ group (p = .008); #Significantly higher than 5% UPJ group (p = .008); n = 9
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4495848&req=5

Fig2: Unripe papaya pulp juice enhances in vitro IL-6 synthesis in SHED in dose dependent manner. A significant increase in IL-6 (a) synthesis was found in the 10% UPJ group compared to the control, 2% UPJ and 5% UPJ groups (p = .008), while the 5% UPJ group produced significantly higher IL-6 compared to control and the 2% UPJ group (p = .008). Synthesis of IL-3 (b), TPO (c) and SCF (d) was not significantly different between groups. *Significantly higher compared to control and 2% UPJ group (p = .008); #Significantly higher than 5% UPJ group (p = .008); n = 9
Mentions: Of the six cytokines measured, only IL-6, IL-3, TPO and SCF were detectable in the assay (Fig. 2), while G-CSF and GM-CSF were below the assay’s level of detection. IL-6 was significantly increased (p = .008) with the addition of 5% and 10% UPJ (Fig. 2a). increase of SCF secretion compared to the control after addition of 5% and 10% UPJ was statistically significant (p = .046 and p = .018 respectively) (Fig. 2d). Concentration of IL-3 declined with the addition of higher concentrations of UPJ (Fig. 2b) while concentration of TPO remained relatively constant across all groups (Fig. 2c).Fig. 2

Bottom Line: Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papaya pulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF) RESULTS: In vitro scratch gap closure was significantly faster (p < .05) in SHED culture treated with UPJ.IL-6 concentration was significantly increased (p < .05) in SHED and PBL culture supernatant when treated with UPJ.SCF synthesis in SHED culture was also significantly increased (p < .05) when treated with UPJ CONCLUSION: In vitro upregulated synthesis of IL -6 and SCF both in PBL and SHED reveals the potential mechanism of unripe papaya to induce thrombopoietic cytokines synthesis in cells of hematopoietic and mesenchymal origin.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, International Islamic University Malaysia, Bandar Indera Mahkota, Kuantan, 25200, Malaysia. jazliaziz87@gmail.com.

ABSTRACT

Background: Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction

Methods: In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papaya pulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF) RESULTS: In vitro scratch gap closure was significantly faster (p < .05) in SHED culture treated with UPJ. IL-6 concentration was significantly increased (p < .05) in SHED and PBL culture supernatant when treated with UPJ. SCF synthesis in SHED culture was also significantly increased (p < .05) when treated with UPJ CONCLUSION: In vitro upregulated synthesis of IL -6 and SCF both in PBL and SHED reveals the potential mechanism of unripe papaya to induce thrombopoietic cytokines synthesis in cells of hematopoietic and mesenchymal origin.

No MeSH data available.


Related in: MedlinePlus