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Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells.

Aziz J, Abu Kassim NL, Abu Kasim NH, Haque N, Rahman MT - BMC Complement Altern Med (2015)

Bottom Line: Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papaya pulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF) RESULTS: In vitro scratch gap closure was significantly faster (p < .05) in SHED culture treated with UPJ.IL-6 concentration was significantly increased (p < .05) in SHED and PBL culture supernatant when treated with UPJ.SCF synthesis in SHED culture was also significantly increased (p < .05) when treated with UPJ CONCLUSION: In vitro upregulated synthesis of IL -6 and SCF both in PBL and SHED reveals the potential mechanism of unripe papaya to induce thrombopoietic cytokines synthesis in cells of hematopoietic and mesenchymal origin.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, International Islamic University Malaysia, Bandar Indera Mahkota, Kuantan, 25200, Malaysia. jazliaziz87@gmail.com.

ABSTRACT

Background: Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction

Methods: In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papaya pulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF) RESULTS: In vitro scratch gap closure was significantly faster (p < .05) in SHED culture treated with UPJ. IL-6 concentration was significantly increased (p < .05) in SHED and PBL culture supernatant when treated with UPJ. SCF synthesis in SHED culture was also significantly increased (p < .05) when treated with UPJ CONCLUSION: In vitro upregulated synthesis of IL -6 and SCF both in PBL and SHED reveals the potential mechanism of unripe papaya to induce thrombopoietic cytokines synthesis in cells of hematopoietic and mesenchymal origin.

No MeSH data available.


Related in: MedlinePlus

Unripe papaya pulp juice promotes gap closure in vitro SHED scratch culture. Addition of 10% UPJ significantly (p = .008) reduced scratch gap width (a) and gap area (b) compared with control groups. Addition of 2% and 5% UPJ did not show any significant difference. Representative photomicrographs of scratch assay of control and 10% UPJ groups at 0 hour (c and e respectively) and 24 hour (d and f respectively). Dotted lines (c and e) show initial scratch. In vitro addition of 2%, 5% and 10% UPJ did not significantly affect SHEDs’ viability (g). *Significantly higher than control group (p = .008); n = 9; [scale bar (C-F): 50 μm]
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Fig1: Unripe papaya pulp juice promotes gap closure in vitro SHED scratch culture. Addition of 10% UPJ significantly (p = .008) reduced scratch gap width (a) and gap area (b) compared with control groups. Addition of 2% and 5% UPJ did not show any significant difference. Representative photomicrographs of scratch assay of control and 10% UPJ groups at 0 hour (c and e respectively) and 24 hour (d and f respectively). Dotted lines (c and e) show initial scratch. In vitro addition of 2%, 5% and 10% UPJ did not significantly affect SHEDs’ viability (g). *Significantly higher than control group (p = .008); n = 9; [scale bar (C-F): 50 μm]

Mentions: The width and surface area of the gap was obtained from the scratch assay to determine the effect of UPJ on SHED proliferation. Both the width (Fig. 1a) and surface area (Fig. 1b) of the gap was significantly reduced with the addition of 10% (v/v) UPJ compared to the control (p = .008). At 72 hours, cell viability in the 10% (v/v) UPJ group was slightly higher compared to control and other treatment groups (Fig. 1g), however the difference was not statistically significant (p = .173).Fig. 1


Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells.

Aziz J, Abu Kassim NL, Abu Kasim NH, Haque N, Rahman MT - BMC Complement Altern Med (2015)

Unripe papaya pulp juice promotes gap closure in vitro SHED scratch culture. Addition of 10% UPJ significantly (p = .008) reduced scratch gap width (a) and gap area (b) compared with control groups. Addition of 2% and 5% UPJ did not show any significant difference. Representative photomicrographs of scratch assay of control and 10% UPJ groups at 0 hour (c and e respectively) and 24 hour (d and f respectively). Dotted lines (c and e) show initial scratch. In vitro addition of 2%, 5% and 10% UPJ did not significantly affect SHEDs’ viability (g). *Significantly higher than control group (p = .008); n = 9; [scale bar (C-F): 50 μm]
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4495848&req=5

Fig1: Unripe papaya pulp juice promotes gap closure in vitro SHED scratch culture. Addition of 10% UPJ significantly (p = .008) reduced scratch gap width (a) and gap area (b) compared with control groups. Addition of 2% and 5% UPJ did not show any significant difference. Representative photomicrographs of scratch assay of control and 10% UPJ groups at 0 hour (c and e respectively) and 24 hour (d and f respectively). Dotted lines (c and e) show initial scratch. In vitro addition of 2%, 5% and 10% UPJ did not significantly affect SHEDs’ viability (g). *Significantly higher than control group (p = .008); n = 9; [scale bar (C-F): 50 μm]
Mentions: The width and surface area of the gap was obtained from the scratch assay to determine the effect of UPJ on SHED proliferation. Both the width (Fig. 1a) and surface area (Fig. 1b) of the gap was significantly reduced with the addition of 10% (v/v) UPJ compared to the control (p = .008). At 72 hours, cell viability in the 10% (v/v) UPJ group was slightly higher compared to control and other treatment groups (Fig. 1g), however the difference was not statistically significant (p = .173).Fig. 1

Bottom Line: Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papaya pulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF) RESULTS: In vitro scratch gap closure was significantly faster (p < .05) in SHED culture treated with UPJ.IL-6 concentration was significantly increased (p < .05) in SHED and PBL culture supernatant when treated with UPJ.SCF synthesis in SHED culture was also significantly increased (p < .05) when treated with UPJ CONCLUSION: In vitro upregulated synthesis of IL -6 and SCF both in PBL and SHED reveals the potential mechanism of unripe papaya to induce thrombopoietic cytokines synthesis in cells of hematopoietic and mesenchymal origin.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, International Islamic University Malaysia, Bandar Indera Mahkota, Kuantan, 25200, Malaysia. jazliaziz87@gmail.com.

ABSTRACT

Background: Use of Carica papaya leaf extracts, reported to improve thrombocyte counts in dengue patients, demands further analysis on the underlying mechanism of its thrombopoietic cytokines induction

Methods: In vitro cultures of peripheral blood leukocytes (PBL) and stem cells from human exfoliated deciduous teeth (SHED) were treated with unripe papaya pulp juice (UPJ) to evaluate its potential to induce thrombopoietic cytokines (IL-6 and SCF) RESULTS: In vitro scratch gap closure was significantly faster (p < .05) in SHED culture treated with UPJ. IL-6 concentration was significantly increased (p < .05) in SHED and PBL culture supernatant when treated with UPJ. SCF synthesis in SHED culture was also significantly increased (p < .05) when treated with UPJ CONCLUSION: In vitro upregulated synthesis of IL -6 and SCF both in PBL and SHED reveals the potential mechanism of unripe papaya to induce thrombopoietic cytokines synthesis in cells of hematopoietic and mesenchymal origin.

No MeSH data available.


Related in: MedlinePlus