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Gastroprotective and ulcer healing effects of piptadeniastrum Africanum on experimentally induced gastric ulcers in rats.

Ateufack G, Domgnim Mokam EC, Mbiantcha M, Dongmo Feudjio RB, David N, Kamanyi A - BMC Complement Altern Med (2015)

Bottom Line: As such, both extracts were found to exhibit preventive and curative effects through the release of NO and growth factors.Piptadeniastrum africanum stem bark extracts thus have gastroprotective and ulcer healing effects, which could result from their activities by stimulating important cellular mechanisms such as migration and proliferation of epithelial cells that may have a cytoprotective effect by stimulating the release of prostaglandins.These results are required to confirm the ethnopharmacological use of Piptadeniastrum africanum stem bark in the treatment of ulcer.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon. ateufack2000@yahoo.fr.

ABSTRACT

Background: Gastric peptic ulcer is one of the common disorders of gastrointestinal tract, which occur due to an imbalance between the offensive and defensive factors. It is an illness that affects a considerable number of people worldwide. This study was conducted to evaluate the antiulcerogenic and antiulcer effects and recognize the basic mechanism of action of Piptadeniastrum africanum stem bark extracts.

Methods: The aqueous and methanol extracts of Piptadeniastrum africanum were administered at the doses 125, 250 and 500 mg/kg to evaluate their effects on gastric ulcer induced by the HCl/ethanol mixture, indomethacin and acetic acid in Wistar strain male adult rats, aged between 12 and 16 weeks and weighing between 180 and 220 g. Ranitidine, Maalox and Misoprostol were used as standard drugs. Histopathological examination and nitric oxide level were performed to evaluate the basic mechanism of action of Piptadeniastrum africanum. Phytochemical screening was carried out to identify known phytochemicals present in these extracts.

Results: The aqueous and methanol extracts of stem bark of Piptadeniastrum africanum significantly inhibited (p < 0.01) gastric ulceration induced by HCl/ethanol to the percentages of inhibition of 81.38; 98.75 and 100 % for the aqueous extract and then 75.83, 89.76 and 96.52 % for the methanol extract, and with the Indomethacin-induced ulcers, aqueous and methanol extracts of bark of Piptadeniastrum africanum reduce significantly (p < 0.01) induced gastric lesions in rats, with percentage of cure 35.75; 52.33 and 98.58 % for the aqueous extract, and 33.7; 51.97; and 65.93 to the methanol extract. The results revealed a significant reduction of ulcerated surface in both extracts and increase of nitric oxide (NO) level with methanol extract. When compared to methanol extract, aqueous extract showed more pronounced effects, corresponding to percentages of healing of 59. 92; 84.12 and 59.65 % for the aqueous extract; and 70.43; 55.49 and 57.59 % for the methanol extract in the ulcer induced by acetic acid, all at the respective doses of 125, 250 and 500 mg/kg. Histopathological observations also demonstrated curative effect. As such, both extracts were found to exhibit preventive and curative effects through the release of NO and growth factors. This could also be due to the presence of phytochemicals such as alkaloids, flavonoids, phenols and saponins which act as antisecretory agents.

Conclusions: Piptadeniastrum africanum stem bark extracts thus have gastroprotective and ulcer healing effects, which could result from their activities by stimulating important cellular mechanisms such as migration and proliferation of epithelial cells that may have a cytoprotective effect by stimulating the release of prostaglandins. These results are required to confirm the ethnopharmacological use of Piptadeniastrum africanum stem bark in the treatment of ulcer.

No MeSH data available.


Related in: MedlinePlus

Gross appearance of the gastric mucosa in rats. a Rats pre-treated with 1ml/100g distilled water (ulcer control). Severe injuries are seen in the gastric mucosa: Hcl/ethanol produced extensive visible hemorrhagic necrosis of gastric mucosa. b & c Rats pre-treated with Maalox and Ranitidine (50 mg/kg) respectively: injuries to the gastric mucosa are milder compared to the injuries seen in the ulcer control rats. d & e Rats pre-treated with aqueous extract at doses 125 and 250 mg/kg respectively: moderate injuries are seen in the gastric mucosa, and the injuries decrease when the dose increase. f Rats pre-treated with aqueous extract at dose 500 mg/kg: no injuries are seen, so at this dose, the aqueous extract completely inhibits the gastric lesions induced by acidified ethanol. g, h & i Rats pre-treated with methanol extract at doses 125, 250 and 500 mg/kg: the injuries reduce with the increase of dose; hence, at 500 mg/kg few injuries are seen. The methanol extract reduces gastric lesions induced by acidified ethanol
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Fig1: Gross appearance of the gastric mucosa in rats. a Rats pre-treated with 1ml/100g distilled water (ulcer control). Severe injuries are seen in the gastric mucosa: Hcl/ethanol produced extensive visible hemorrhagic necrosis of gastric mucosa. b & c Rats pre-treated with Maalox and Ranitidine (50 mg/kg) respectively: injuries to the gastric mucosa are milder compared to the injuries seen in the ulcer control rats. d & e Rats pre-treated with aqueous extract at doses 125 and 250 mg/kg respectively: moderate injuries are seen in the gastric mucosa, and the injuries decrease when the dose increase. f Rats pre-treated with aqueous extract at dose 500 mg/kg: no injuries are seen, so at this dose, the aqueous extract completely inhibits the gastric lesions induced by acidified ethanol. g, h & i Rats pre-treated with methanol extract at doses 125, 250 and 500 mg/kg: the injuries reduce with the increase of dose; hence, at 500 mg/kg few injuries are seen. The methanol extract reduces gastric lesions induced by acidified ethanol

Mentions: The oral administration of HCl/ethanol solution produced characteristic lesions in the glandular portion of the negative control rat stomach with a total surface area of 236.54 ± 1.73 mm2. The aqueous extract of Piptadeniastrum africanum produced a dose-dependent inhibition of gastric ulceration ranging from 81.38 % at a dose of 125 mg/kg to 100 % at a dose of 500 mg/kg with ulcer surface areas of 58.86 ± 5.33 and 0.00 ± 0.00 mm2 respectively. The methanol extract also produced 75.83 % inhibition at a dose of 125 mg/kg and 96.52 % inhibition at a dose of 500 mg/kg. The corresponding ulcer surface areas were 85.74 ± 5.08 and 8.23 ± 2.47mm2. Animals treated with Maalox and Ranitidine at a dose 50mg/kg produced a significant (p < 0.01) decrease in ulcer surface area from 236.54 ± 1.73 mm2 to 67.74 ± 4.41 and 66.66 ± 9.78 mm2 respectively (Table 1). Macroscopic observation (Fig. 1) showed the gastric mucosal of rats.Table 1


Gastroprotective and ulcer healing effects of piptadeniastrum Africanum on experimentally induced gastric ulcers in rats.

Ateufack G, Domgnim Mokam EC, Mbiantcha M, Dongmo Feudjio RB, David N, Kamanyi A - BMC Complement Altern Med (2015)

Gross appearance of the gastric mucosa in rats. a Rats pre-treated with 1ml/100g distilled water (ulcer control). Severe injuries are seen in the gastric mucosa: Hcl/ethanol produced extensive visible hemorrhagic necrosis of gastric mucosa. b & c Rats pre-treated with Maalox and Ranitidine (50 mg/kg) respectively: injuries to the gastric mucosa are milder compared to the injuries seen in the ulcer control rats. d & e Rats pre-treated with aqueous extract at doses 125 and 250 mg/kg respectively: moderate injuries are seen in the gastric mucosa, and the injuries decrease when the dose increase. f Rats pre-treated with aqueous extract at dose 500 mg/kg: no injuries are seen, so at this dose, the aqueous extract completely inhibits the gastric lesions induced by acidified ethanol. g, h & i Rats pre-treated with methanol extract at doses 125, 250 and 500 mg/kg: the injuries reduce with the increase of dose; hence, at 500 mg/kg few injuries are seen. The methanol extract reduces gastric lesions induced by acidified ethanol
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4495702&req=5

Fig1: Gross appearance of the gastric mucosa in rats. a Rats pre-treated with 1ml/100g distilled water (ulcer control). Severe injuries are seen in the gastric mucosa: Hcl/ethanol produced extensive visible hemorrhagic necrosis of gastric mucosa. b & c Rats pre-treated with Maalox and Ranitidine (50 mg/kg) respectively: injuries to the gastric mucosa are milder compared to the injuries seen in the ulcer control rats. d & e Rats pre-treated with aqueous extract at doses 125 and 250 mg/kg respectively: moderate injuries are seen in the gastric mucosa, and the injuries decrease when the dose increase. f Rats pre-treated with aqueous extract at dose 500 mg/kg: no injuries are seen, so at this dose, the aqueous extract completely inhibits the gastric lesions induced by acidified ethanol. g, h & i Rats pre-treated with methanol extract at doses 125, 250 and 500 mg/kg: the injuries reduce with the increase of dose; hence, at 500 mg/kg few injuries are seen. The methanol extract reduces gastric lesions induced by acidified ethanol
Mentions: The oral administration of HCl/ethanol solution produced characteristic lesions in the glandular portion of the negative control rat stomach with a total surface area of 236.54 ± 1.73 mm2. The aqueous extract of Piptadeniastrum africanum produced a dose-dependent inhibition of gastric ulceration ranging from 81.38 % at a dose of 125 mg/kg to 100 % at a dose of 500 mg/kg with ulcer surface areas of 58.86 ± 5.33 and 0.00 ± 0.00 mm2 respectively. The methanol extract also produced 75.83 % inhibition at a dose of 125 mg/kg and 96.52 % inhibition at a dose of 500 mg/kg. The corresponding ulcer surface areas were 85.74 ± 5.08 and 8.23 ± 2.47mm2. Animals treated with Maalox and Ranitidine at a dose 50mg/kg produced a significant (p < 0.01) decrease in ulcer surface area from 236.54 ± 1.73 mm2 to 67.74 ± 4.41 and 66.66 ± 9.78 mm2 respectively (Table 1). Macroscopic observation (Fig. 1) showed the gastric mucosal of rats.Table 1

Bottom Line: As such, both extracts were found to exhibit preventive and curative effects through the release of NO and growth factors.Piptadeniastrum africanum stem bark extracts thus have gastroprotective and ulcer healing effects, which could result from their activities by stimulating important cellular mechanisms such as migration and proliferation of epithelial cells that may have a cytoprotective effect by stimulating the release of prostaglandins.These results are required to confirm the ethnopharmacological use of Piptadeniastrum africanum stem bark in the treatment of ulcer.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon. ateufack2000@yahoo.fr.

ABSTRACT

Background: Gastric peptic ulcer is one of the common disorders of gastrointestinal tract, which occur due to an imbalance between the offensive and defensive factors. It is an illness that affects a considerable number of people worldwide. This study was conducted to evaluate the antiulcerogenic and antiulcer effects and recognize the basic mechanism of action of Piptadeniastrum africanum stem bark extracts.

Methods: The aqueous and methanol extracts of Piptadeniastrum africanum were administered at the doses 125, 250 and 500 mg/kg to evaluate their effects on gastric ulcer induced by the HCl/ethanol mixture, indomethacin and acetic acid in Wistar strain male adult rats, aged between 12 and 16 weeks and weighing between 180 and 220 g. Ranitidine, Maalox and Misoprostol were used as standard drugs. Histopathological examination and nitric oxide level were performed to evaluate the basic mechanism of action of Piptadeniastrum africanum. Phytochemical screening was carried out to identify known phytochemicals present in these extracts.

Results: The aqueous and methanol extracts of stem bark of Piptadeniastrum africanum significantly inhibited (p < 0.01) gastric ulceration induced by HCl/ethanol to the percentages of inhibition of 81.38; 98.75 and 100 % for the aqueous extract and then 75.83, 89.76 and 96.52 % for the methanol extract, and with the Indomethacin-induced ulcers, aqueous and methanol extracts of bark of Piptadeniastrum africanum reduce significantly (p < 0.01) induced gastric lesions in rats, with percentage of cure 35.75; 52.33 and 98.58 % for the aqueous extract, and 33.7; 51.97; and 65.93 to the methanol extract. The results revealed a significant reduction of ulcerated surface in both extracts and increase of nitric oxide (NO) level with methanol extract. When compared to methanol extract, aqueous extract showed more pronounced effects, corresponding to percentages of healing of 59. 92; 84.12 and 59.65 % for the aqueous extract; and 70.43; 55.49 and 57.59 % for the methanol extract in the ulcer induced by acetic acid, all at the respective doses of 125, 250 and 500 mg/kg. Histopathological observations also demonstrated curative effect. As such, both extracts were found to exhibit preventive and curative effects through the release of NO and growth factors. This could also be due to the presence of phytochemicals such as alkaloids, flavonoids, phenols and saponins which act as antisecretory agents.

Conclusions: Piptadeniastrum africanum stem bark extracts thus have gastroprotective and ulcer healing effects, which could result from their activities by stimulating important cellular mechanisms such as migration and proliferation of epithelial cells that may have a cytoprotective effect by stimulating the release of prostaglandins. These results are required to confirm the ethnopharmacological use of Piptadeniastrum africanum stem bark in the treatment of ulcer.

No MeSH data available.


Related in: MedlinePlus