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Effect of N-acetylcysteine and allopurinol combination to protect spinal cord ischemia/reperfusion injury induced by aortic cross-clamping in rat model.

Erkut B, Onk OA - J Cardiothorac Surg (2015)

Bottom Line: The experimental group subjects had better neurological functions than control group subjects.In experimental group; superoxide dismutase and catalase levels increased, while malondialdehyde and xantine oxidase levels decreased as compared with control group.Histopathological examination showed that experimental group had less cell degeneration, hemorrhage, edema and inflammation loss than control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Surgery, Erzincan University Medical Faculty, Training and Research Hospital, Erzincan, Turkey. bilgehanerkut@yahoo.com.

ABSTRACT

Purpose: The aim of this experimental study was to determine whether combination of N-acetylcysteine and allopurinol can reduce the ischemia/reperfusion injury of spinal cord in a rat model.

Methods: Twenty-seven Spraque Dawley rats, all male, weighing between 220 to 370 (mean 325) gr were used in the study. 27 rats were divided into three groups: sham group, control group and experimental group. Abdominal aortic occlusion between the renal arteries and iliac bifurcations was carried out for 60 min with proximal and distal clip in control and experimental groups. Hindlimb motor functions were evaluated at 24, and 48 h using the Tarlov Scale. Besides, spinal cord samples were taken for determination of superoxide dismutase, and catalase activities as antioxidant enzymes, and malondialdehyde as an indicator of lipid peroxidation and xanthine oxidase levels as source hydroxyl radical for biochemical studies. Also, histopathological evaluation was made from cord tissue samples.

Results: The experimental group subjects had better neurological functions than control group subjects. In experimental group; superoxide dismutase and catalase levels increased, while malondialdehyde and xantine oxidase levels decreased as compared with control group. Histopathological examination showed that experimental group had less cell degeneration, hemorrhage, edema and inflammation loss than control group.

Conclusions: This study offers that combined use of N-acetylcysteine and allopurinol might help protect the spinal cord against ischemia/reperfusion injury.

No MeSH data available.


Related in: MedlinePlus

Effects of NAC and allopurinol on spinal cord tissue. Between groups antioxidant enzyme levels. a, b Increased SOD and CAT levels in group experimental in comparison to control group. c, d Decreased MDA and XO levels in experimental group in comparison to control group. SOD, superoxide dismutase; CAT, catalase; MDA, malondialdehyde; XO, xanthine oxidase
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Fig3: Effects of NAC and allopurinol on spinal cord tissue. Between groups antioxidant enzyme levels. a, b Increased SOD and CAT levels in group experimental in comparison to control group. c, d Decreased MDA and XO levels in experimental group in comparison to control group. SOD, superoxide dismutase; CAT, catalase; MDA, malondialdehyde; XO, xanthine oxidase

Mentions: SOD, CAT, MDA, and XO levels were measured in spinal cord tissue specimens after 48 h reperfusion. SOD and CAT levels increased in the experimental groups compared to control group (from 0,10 ± 0,07 to 0,33 ± 0,05 for SOD; from 6,96 ± 0.90 to 12,52 ± 0,80 for CAT), and and it was significant as statistically (p < 0.05) (Fig. 3a and Fig. 3b). XO levels in the spinal cord were found to be higher in the control group. However, in treatment groups lowered (from 5,11 ± 0,55 to 1,71 ± 0,44) the levels of XO Tissue MDA levels were decreased (from 2,92 ± 0,09 to 0,61 ± 0,08) in the treatment group compared to the control group. The results of SOD, CAT, MDA, and XO assays are shown in Fig. 3a–d.Fig. 3


Effect of N-acetylcysteine and allopurinol combination to protect spinal cord ischemia/reperfusion injury induced by aortic cross-clamping in rat model.

Erkut B, Onk OA - J Cardiothorac Surg (2015)

Effects of NAC and allopurinol on spinal cord tissue. Between groups antioxidant enzyme levels. a, b Increased SOD and CAT levels in group experimental in comparison to control group. c, d Decreased MDA and XO levels in experimental group in comparison to control group. SOD, superoxide dismutase; CAT, catalase; MDA, malondialdehyde; XO, xanthine oxidase
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4495695&req=5

Fig3: Effects of NAC and allopurinol on spinal cord tissue. Between groups antioxidant enzyme levels. a, b Increased SOD and CAT levels in group experimental in comparison to control group. c, d Decreased MDA and XO levels in experimental group in comparison to control group. SOD, superoxide dismutase; CAT, catalase; MDA, malondialdehyde; XO, xanthine oxidase
Mentions: SOD, CAT, MDA, and XO levels were measured in spinal cord tissue specimens after 48 h reperfusion. SOD and CAT levels increased in the experimental groups compared to control group (from 0,10 ± 0,07 to 0,33 ± 0,05 for SOD; from 6,96 ± 0.90 to 12,52 ± 0,80 for CAT), and and it was significant as statistically (p < 0.05) (Fig. 3a and Fig. 3b). XO levels in the spinal cord were found to be higher in the control group. However, in treatment groups lowered (from 5,11 ± 0,55 to 1,71 ± 0,44) the levels of XO Tissue MDA levels were decreased (from 2,92 ± 0,09 to 0,61 ± 0,08) in the treatment group compared to the control group. The results of SOD, CAT, MDA, and XO assays are shown in Fig. 3a–d.Fig. 3

Bottom Line: The experimental group subjects had better neurological functions than control group subjects.In experimental group; superoxide dismutase and catalase levels increased, while malondialdehyde and xantine oxidase levels decreased as compared with control group.Histopathological examination showed that experimental group had less cell degeneration, hemorrhage, edema and inflammation loss than control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Surgery, Erzincan University Medical Faculty, Training and Research Hospital, Erzincan, Turkey. bilgehanerkut@yahoo.com.

ABSTRACT

Purpose: The aim of this experimental study was to determine whether combination of N-acetylcysteine and allopurinol can reduce the ischemia/reperfusion injury of spinal cord in a rat model.

Methods: Twenty-seven Spraque Dawley rats, all male, weighing between 220 to 370 (mean 325) gr were used in the study. 27 rats were divided into three groups: sham group, control group and experimental group. Abdominal aortic occlusion between the renal arteries and iliac bifurcations was carried out for 60 min with proximal and distal clip in control and experimental groups. Hindlimb motor functions were evaluated at 24, and 48 h using the Tarlov Scale. Besides, spinal cord samples were taken for determination of superoxide dismutase, and catalase activities as antioxidant enzymes, and malondialdehyde as an indicator of lipid peroxidation and xanthine oxidase levels as source hydroxyl radical for biochemical studies. Also, histopathological evaluation was made from cord tissue samples.

Results: The experimental group subjects had better neurological functions than control group subjects. In experimental group; superoxide dismutase and catalase levels increased, while malondialdehyde and xantine oxidase levels decreased as compared with control group. Histopathological examination showed that experimental group had less cell degeneration, hemorrhage, edema and inflammation loss than control group.

Conclusions: This study offers that combined use of N-acetylcysteine and allopurinol might help protect the spinal cord against ischemia/reperfusion injury.

No MeSH data available.


Related in: MedlinePlus