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Relationship between circulating syndecan-1 levels (CD138s) and serum free light chains in monoclonal gammopathies.

Cigliana G, Torti E, Gulli F, De Santis E, Dell'Abate MT, Colacicco L, Pisani F, Conti L, Basile U - J. Exp. Clin. Cancer Res. (2015)

Bottom Line: We observed higher CD138 mean values in myeloma patients compared to the light chain only myeloma group.A positive linear regression of CD138 and FLC was observed in the light chain only cohort as opposed to myeloma patients which show an inverse trend.The study highlighted an existing relationship between FLCs and CD138 and wishes to seek also a correlation in order to rapidly and efficiently perform diagnosis and different diagnostic schemes.

View Article: PubMed Central - PubMed

Affiliation: Department of Prevention and Diagnostic Oncology, Laboratory of Clinical Pathology -National Cancer Institute "Regina Elena", Rome, Italy. cigliana@ifo.it.

ABSTRACT

Background: Monoclonal gammopathies encompass a wide range of diseases characterized by the monoclonal expansion of a B-cell clone. Despite emerging therapeutic strategies, chances of survival of patients who are affected are still scarce, which implies that new tools are necessary not only for the diagnosis but also for the follow-up of patients affected by such diseases. In this context, the use of free light chains (FLCs) has been incorporated into many guidelines. Likewise, tumor microenvironment is consistently gaining importance as role player in tumor pathogenesis. Specifically, Syndecan-1 (CD138), a heparan-sulfate proteoglycan is attracting interests as it is highly expressed and shed by myeloma plasma-cells. The aim of our study was to analyze CD138 levels in the serum of patients affected by multiple myeloma or light chain only disease, and to compare the values obtained with free light chain (FLC) kappa, lambda and FLC ratio in both groups of patients.

Methods: 84 patients affected by Multiple Myeloma and Light Chain Myeloma were recruited for this study. Serum CD138 was assessed by ELISA (Diaclone Research, France) and FLC values were quantified by nephelometry (Freelite TM Human Kappa and Lambda Free Kits, The Binding Site, UK). Data was analyzed by GraphPad Prism software and Statgraph.

Results: We observed higher CD138 mean values in myeloma patients compared to the light chain only myeloma group. A positive linear regression of CD138 and FLC was observed in the light chain only cohort as opposed to myeloma patients which show an inverse trend.

Conclusions: The study highlighted an existing relationship between FLCs and CD138 and wishes to seek also a correlation in order to rapidly and efficiently perform diagnosis and different diagnostic schemes.

No MeSH data available.


Related in: MedlinePlus

Scatter graph and correlations (R) of serum CD138 concentrations versus Serum Free Light Chain Kappa or Lambda concentrations in Intact Immunoglobulin Multiple Myeloma and Light Chain Myeloma Patients. R values were calculated by Pearson’s correlation.
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Fig1: Scatter graph and correlations (R) of serum CD138 concentrations versus Serum Free Light Chain Kappa or Lambda concentrations in Intact Immunoglobulin Multiple Myeloma and Light Chain Myeloma Patients. R values were calculated by Pearson’s correlation.

Mentions: Analysis of linear correlations was performed by means of Pearson’s correlation, in order to assess the degree of association of the two variables. Correlation coefficients are reported in Figure 1. ROC curves (Receiver Operating Characteristic or Relative Operating Characteristic curves) were generated by plotting data (CD138 values of patients against CD138 values of healthy donors) on GraphPad and the resulting information was then re-plotted on Excel (Figure 2). Data from serum CD138 concentrations in healthy subjects were plotted against values derived from the two groups of patients to give estimates of true positive values (sensitivity) and the proportion of false negatives (specificity). Plotted values are represented as a curve, and the Area Under the Curve (AUC) is indicative of diagnostic accuracy. An AUC = 1 (100%) denotes full accuracy of the test. In this way, it is possible to discriminate normal from abnormal values, which give an estimate of a cut-off value for a specific test in a particular setting. Usually, an AUC = 80% is considered as optimal value. Results were deemed statistically significant for P < 0.05.Figure 1


Relationship between circulating syndecan-1 levels (CD138s) and serum free light chains in monoclonal gammopathies.

Cigliana G, Torti E, Gulli F, De Santis E, Dell'Abate MT, Colacicco L, Pisani F, Conti L, Basile U - J. Exp. Clin. Cancer Res. (2015)

Scatter graph and correlations (R) of serum CD138 concentrations versus Serum Free Light Chain Kappa or Lambda concentrations in Intact Immunoglobulin Multiple Myeloma and Light Chain Myeloma Patients. R values were calculated by Pearson’s correlation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4495691&req=5

Fig1: Scatter graph and correlations (R) of serum CD138 concentrations versus Serum Free Light Chain Kappa or Lambda concentrations in Intact Immunoglobulin Multiple Myeloma and Light Chain Myeloma Patients. R values were calculated by Pearson’s correlation.
Mentions: Analysis of linear correlations was performed by means of Pearson’s correlation, in order to assess the degree of association of the two variables. Correlation coefficients are reported in Figure 1. ROC curves (Receiver Operating Characteristic or Relative Operating Characteristic curves) were generated by plotting data (CD138 values of patients against CD138 values of healthy donors) on GraphPad and the resulting information was then re-plotted on Excel (Figure 2). Data from serum CD138 concentrations in healthy subjects were plotted against values derived from the two groups of patients to give estimates of true positive values (sensitivity) and the proportion of false negatives (specificity). Plotted values are represented as a curve, and the Area Under the Curve (AUC) is indicative of diagnostic accuracy. An AUC = 1 (100%) denotes full accuracy of the test. In this way, it is possible to discriminate normal from abnormal values, which give an estimate of a cut-off value for a specific test in a particular setting. Usually, an AUC = 80% is considered as optimal value. Results were deemed statistically significant for P < 0.05.Figure 1

Bottom Line: We observed higher CD138 mean values in myeloma patients compared to the light chain only myeloma group.A positive linear regression of CD138 and FLC was observed in the light chain only cohort as opposed to myeloma patients which show an inverse trend.The study highlighted an existing relationship between FLCs and CD138 and wishes to seek also a correlation in order to rapidly and efficiently perform diagnosis and different diagnostic schemes.

View Article: PubMed Central - PubMed

Affiliation: Department of Prevention and Diagnostic Oncology, Laboratory of Clinical Pathology -National Cancer Institute "Regina Elena", Rome, Italy. cigliana@ifo.it.

ABSTRACT

Background: Monoclonal gammopathies encompass a wide range of diseases characterized by the monoclonal expansion of a B-cell clone. Despite emerging therapeutic strategies, chances of survival of patients who are affected are still scarce, which implies that new tools are necessary not only for the diagnosis but also for the follow-up of patients affected by such diseases. In this context, the use of free light chains (FLCs) has been incorporated into many guidelines. Likewise, tumor microenvironment is consistently gaining importance as role player in tumor pathogenesis. Specifically, Syndecan-1 (CD138), a heparan-sulfate proteoglycan is attracting interests as it is highly expressed and shed by myeloma plasma-cells. The aim of our study was to analyze CD138 levels in the serum of patients affected by multiple myeloma or light chain only disease, and to compare the values obtained with free light chain (FLC) kappa, lambda and FLC ratio in both groups of patients.

Methods: 84 patients affected by Multiple Myeloma and Light Chain Myeloma were recruited for this study. Serum CD138 was assessed by ELISA (Diaclone Research, France) and FLC values were quantified by nephelometry (Freelite TM Human Kappa and Lambda Free Kits, The Binding Site, UK). Data was analyzed by GraphPad Prism software and Statgraph.

Results: We observed higher CD138 mean values in myeloma patients compared to the light chain only myeloma group. A positive linear regression of CD138 and FLC was observed in the light chain only cohort as opposed to myeloma patients which show an inverse trend.

Conclusions: The study highlighted an existing relationship between FLCs and CD138 and wishes to seek also a correlation in order to rapidly and efficiently perform diagnosis and different diagnostic schemes.

No MeSH data available.


Related in: MedlinePlus