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Effect of Ampelopsis Radix on wound healing in scalded rats.

Lee K, Lee B, Lee MH, Kim B, Chinannai KS, Ham I, Choi HY - BMC Complement Altern Med (2015)

Bottom Line: Histopathological evaluation showed a well-organized epithelial layer, angiogenesis, tissue granulation and collagen formation with the exception of inflammatory cells in the AJE-treated groups compared to the control groups on day 14, indicating that tissue regeneration had occurred.AJE treatment decreased TNF-α and increased IL-10 levels on days 2 and 14, indicating the anti-inflammatory action of AJE.These results suggest that AJE possesses scald wound healing activity via accelerating the scald wound repair during the inflammation and proliferative phases of the healing process.

View Article: PubMed Central - PubMed

Affiliation: Department of Herbology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 130-701, Republic of Korea. dostudy@naver.com.

ABSTRACT

Background: Ampelopsis Radix has been used as a traditional Korean medicine for the treatment of burns and scalds. However, there has been no scientific research to date on the wound healing properties of Ampelopsis Radix for scald burns. This study aimed to evaluate the healing effect of Ampelopsis japonica root tuber ethanol extract (AJE) on induced cutaneous scald injury in Sprague Dawley (SD) rats.

Methods: Hot water scalds were induced in SD rats, who were then divided into the following 5 groups; 1) control group without treatment, 2) positive control group with 1% Silver sulfadiazine (SSD), 3) Vaseline group, and groups 4) and 5) that used Vaseline containing 5% and 20% AJE, respectively. The ointment was applied topically to the experimental rats, once daily for 21 days, starting at 24 h post induction of the scald injury. Gross examination, measurement of wound size, and histopathological examination were performed. And quantitative measurement of cytokine levels of tumor necrosis factor alpha (TNF-α), interleukin-10 (IL-10), transforming growth factor beta 1 (TGF-β1), and vascular endothelial growth factor (VEGF) were performed by enzyme-linked immunosorbent assay.

Results: Clinical evaluation showed that the AJE and Vaseline groups, rapidly desquamated scab on day 12 post-scalding; in particular, the 20% AJE group achieved the greatest extent of skin recovery. Sizes of scald wound were significantly lower on days 12, 15, 18, and 21 in the AJE treated groups compared to the control groups. Histopathological evaluation showed a well-organized epithelial layer, angiogenesis, tissue granulation and collagen formation with the exception of inflammatory cells in the AJE-treated groups compared to the control groups on day 14, indicating that tissue regeneration had occurred. AJE treatment decreased TNF-α and increased IL-10 levels on days 2 and 14, indicating the anti-inflammatory action of AJE. The AJE groups also showed a decrease in TGF-β1 levels on day 7 and VEGF on day 14 in the serum of scald inflicted SD rat model.

Conclusions: These results suggest that AJE possesses scald wound healing activity via accelerating the scald wound repair during the inflammation and proliferative phases of the healing process.

No MeSH data available.


Related in: MedlinePlus

IL-10 levels in cutaneous scald injury. Abbreviations: CON, control; SSD, silver sulfadiazine; AJE, Ampelopsis japonica tuberous root ethanol extract; IL-10, interleukin-10. Values expressed as mean ± standard error of the mean (n = 4–8). ***P < 0.001 vs. control. #P < 0.05 vs. Vaseline
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Fig6: IL-10 levels in cutaneous scald injury. Abbreviations: CON, control; SSD, silver sulfadiazine; AJE, Ampelopsis japonica tuberous root ethanol extract; IL-10, interleukin-10. Values expressed as mean ± standard error of the mean (n = 4–8). ***P < 0.001 vs. control. #P < 0.05 vs. Vaseline

Mentions: TNF-α, a pro-inflammatory cytokine, is up-regulated during the inflammatory phase of wound healing [28] and appears to be involved in initiating the early wound healing response [29]. Low levels of TNF-α can promote wound healing indirectly but high levels of TNF-α can delay wound healing [28]. Therefore up-regulated TNF-α during the inflammatory phase should decreased for the rapid wound healing. IL-10 is an anti-inflammatory cytokine produced by various cells, including macrophages and T-lymphocytes, and is also involved in angiogenesis [30]. It is known to be a major regulator in suppressing the inflammatory response. IL-10 inhibits the synthesis of pro-inflammatory cytokines such as IL-1B, IL-6 and TNF-α in activated macrophages [31]. In the present study, serum samples were collected on days 2 and 14 to determine the impact of the scald wound on the pro-inflammatory response. On day 2, the levels of TNF-α were higher in the 5 % and 20 % AJE treated group compared to the control group and Vaseline treated group. On day 14, TNF-α level was decreased in all groups and TNF-α level was lower in the 20 % AJE treated group than Vaseline treated group (Fig. 5). On the other hand, the IL-10 levels were higher on day 14 in AJE treated groups than in the control group and Vaseline treated group. On day 21, all experimental groups were maintained higher levels of IL-10 than the control group throughout the proliferative phase (Fig. 6). In addition, the histopathological sections of the dorsal skin on day 14 in the post-scald AJE treatment groups showed a lower number of inflammatory cells than the control group (Table 1). These result suggested that AJE can reduce wound size and promote wound healing via decreasing of up-regulated TNF-α levels and Increasing of IL-10.Fig. 5


Effect of Ampelopsis Radix on wound healing in scalded rats.

Lee K, Lee B, Lee MH, Kim B, Chinannai KS, Ham I, Choi HY - BMC Complement Altern Med (2015)

IL-10 levels in cutaneous scald injury. Abbreviations: CON, control; SSD, silver sulfadiazine; AJE, Ampelopsis japonica tuberous root ethanol extract; IL-10, interleukin-10. Values expressed as mean ± standard error of the mean (n = 4–8). ***P < 0.001 vs. control. #P < 0.05 vs. Vaseline
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4495638&req=5

Fig6: IL-10 levels in cutaneous scald injury. Abbreviations: CON, control; SSD, silver sulfadiazine; AJE, Ampelopsis japonica tuberous root ethanol extract; IL-10, interleukin-10. Values expressed as mean ± standard error of the mean (n = 4–8). ***P < 0.001 vs. control. #P < 0.05 vs. Vaseline
Mentions: TNF-α, a pro-inflammatory cytokine, is up-regulated during the inflammatory phase of wound healing [28] and appears to be involved in initiating the early wound healing response [29]. Low levels of TNF-α can promote wound healing indirectly but high levels of TNF-α can delay wound healing [28]. Therefore up-regulated TNF-α during the inflammatory phase should decreased for the rapid wound healing. IL-10 is an anti-inflammatory cytokine produced by various cells, including macrophages and T-lymphocytes, and is also involved in angiogenesis [30]. It is known to be a major regulator in suppressing the inflammatory response. IL-10 inhibits the synthesis of pro-inflammatory cytokines such as IL-1B, IL-6 and TNF-α in activated macrophages [31]. In the present study, serum samples were collected on days 2 and 14 to determine the impact of the scald wound on the pro-inflammatory response. On day 2, the levels of TNF-α were higher in the 5 % and 20 % AJE treated group compared to the control group and Vaseline treated group. On day 14, TNF-α level was decreased in all groups and TNF-α level was lower in the 20 % AJE treated group than Vaseline treated group (Fig. 5). On the other hand, the IL-10 levels were higher on day 14 in AJE treated groups than in the control group and Vaseline treated group. On day 21, all experimental groups were maintained higher levels of IL-10 than the control group throughout the proliferative phase (Fig. 6). In addition, the histopathological sections of the dorsal skin on day 14 in the post-scald AJE treatment groups showed a lower number of inflammatory cells than the control group (Table 1). These result suggested that AJE can reduce wound size and promote wound healing via decreasing of up-regulated TNF-α levels and Increasing of IL-10.Fig. 5

Bottom Line: Histopathological evaluation showed a well-organized epithelial layer, angiogenesis, tissue granulation and collagen formation with the exception of inflammatory cells in the AJE-treated groups compared to the control groups on day 14, indicating that tissue regeneration had occurred.AJE treatment decreased TNF-α and increased IL-10 levels on days 2 and 14, indicating the anti-inflammatory action of AJE.These results suggest that AJE possesses scald wound healing activity via accelerating the scald wound repair during the inflammation and proliferative phases of the healing process.

View Article: PubMed Central - PubMed

Affiliation: Department of Herbology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 130-701, Republic of Korea. dostudy@naver.com.

ABSTRACT

Background: Ampelopsis Radix has been used as a traditional Korean medicine for the treatment of burns and scalds. However, there has been no scientific research to date on the wound healing properties of Ampelopsis Radix for scald burns. This study aimed to evaluate the healing effect of Ampelopsis japonica root tuber ethanol extract (AJE) on induced cutaneous scald injury in Sprague Dawley (SD) rats.

Methods: Hot water scalds were induced in SD rats, who were then divided into the following 5 groups; 1) control group without treatment, 2) positive control group with 1% Silver sulfadiazine (SSD), 3) Vaseline group, and groups 4) and 5) that used Vaseline containing 5% and 20% AJE, respectively. The ointment was applied topically to the experimental rats, once daily for 21 days, starting at 24 h post induction of the scald injury. Gross examination, measurement of wound size, and histopathological examination were performed. And quantitative measurement of cytokine levels of tumor necrosis factor alpha (TNF-α), interleukin-10 (IL-10), transforming growth factor beta 1 (TGF-β1), and vascular endothelial growth factor (VEGF) were performed by enzyme-linked immunosorbent assay.

Results: Clinical evaluation showed that the AJE and Vaseline groups, rapidly desquamated scab on day 12 post-scalding; in particular, the 20% AJE group achieved the greatest extent of skin recovery. Sizes of scald wound were significantly lower on days 12, 15, 18, and 21 in the AJE treated groups compared to the control groups. Histopathological evaluation showed a well-organized epithelial layer, angiogenesis, tissue granulation and collagen formation with the exception of inflammatory cells in the AJE-treated groups compared to the control groups on day 14, indicating that tissue regeneration had occurred. AJE treatment decreased TNF-α and increased IL-10 levels on days 2 and 14, indicating the anti-inflammatory action of AJE. The AJE groups also showed a decrease in TGF-β1 levels on day 7 and VEGF on day 14 in the serum of scald inflicted SD rat model.

Conclusions: These results suggest that AJE possesses scald wound healing activity via accelerating the scald wound repair during the inflammation and proliferative phases of the healing process.

No MeSH data available.


Related in: MedlinePlus