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Mesenchymal stromal cells for cutaneous wound healing in a rabbit model: pre-clinical study applicable in the pediatric surgical setting.

Pelizzo G, Avanzini MA, Icaro Cornaglia A, Osti M, Romano P, Avolio L, Maccario R, Dominici M, De Silvestri A, Andreatta E, Costanzo F, Mantelli M, Ingo D, Piccinno S, Calcaterra V - J Transl Med (2015)

Bottom Line: Rabbit ASCs were isolated and expanded in vitro with relative abundance, cells expressed typical surface markers (CD49e, CD90 and CD29).Improved re-epithelization, reduced inflammatory infiltration and increased collagen deposition were observed in biopsies from wounds treated with ASCs, with the best result in the autologous setting.Pre-clinical studies are however necessary to validate the best skin regeneration technique, which could be used in pediatric surgical translational research.

View Article: PubMed Central - PubMed

Affiliation: Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo and University of Pavia, 27100, Pavia, Italy. g.pelizzo@smatteo.pv.it.

ABSTRACT

Objective: Mesenchymal stromal cells (MSCs) expanded in vitro have been proposed as a potential therapy for congenital or acquired skin defects in pediatrics. The aim of this pre-clinical study was to investigate the effects of intradermal injections of MSC in experimental cutaneous wound repair comparing allogeneic and autologous adipose stem cells (ASCs) and autologous bone marrow-mesenchymal stromal cells (BM-MSCs).

Methods: Mesenchymal stromal cells were in vitro expanded from adipose and BM tissues of young female New Zealand rabbits. MSCs were characterized for plastic adhesion, surface markers, proliferation and differentiation capacity. When an adequate number of cells (ASCs 10 × 10(6) and BM-MSCs 3 × 10(6), because of their low rate of proliferation) was reached, two skin wounds were surgically induced in each animal. The first was topically treated with cell infusions, the second was used as a control. The intradermal inoculation included autologous or allogeneic ASCs or autologous BM-MSCs. For histological examination, animals were sacrificed and wounds were harvested after 11 and 21 days of treatment.

Results: Rabbit ASCs were isolated and expanded in vitro with relative abundance, cells expressed typical surface markers (CD49e, CD90 and CD29). Topically, ASC inoculation provided more rapid wound healing than BM-MSCs and controls. Improved re-epithelization, reduced inflammatory infiltration and increased collagen deposition were observed in biopsies from wounds treated with ASCs, with the best result in the autologous setting. ASCs also improved restoration of skin architecture during wound healing.

Conclusion: The use of ASCs may offer a promising solution to treat extended wounds. Pre-clinical studies are however necessary to validate the best skin regeneration technique, which could be used in pediatric surgical translational research.

No MeSH data available.


Related in: MedlinePlus

Experimental design. Rabbit ASCs and BM-MSCs were collected, isolated and expanded. As soon as an adequate number of in vitro expanded MSCs was reached, two wound lesions (wound 1 and wound 2) were created. Within 5 min from the wound lesion establishment, autologous or allogeneic ASCs or autologous BM-MSCs were directly injected into the wound bed 1. Into wound bed 2, 3 ml of saline 2% rabbit albumin solution was injected as a control. Wound healing was monitored daily. After rabbit euthanasia, biopsies of the regenerated tissues were collected, using dermal biopsy punches, for histological examination.
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Fig1: Experimental design. Rabbit ASCs and BM-MSCs were collected, isolated and expanded. As soon as an adequate number of in vitro expanded MSCs was reached, two wound lesions (wound 1 and wound 2) were created. Within 5 min from the wound lesion establishment, autologous or allogeneic ASCs or autologous BM-MSCs were directly injected into the wound bed 1. Into wound bed 2, 3 ml of saline 2% rabbit albumin solution was injected as a control. Wound healing was monitored daily. After rabbit euthanasia, biopsies of the regenerated tissues were collected, using dermal biopsy punches, for histological examination.

Mentions: For the experimental outline see Figure 1.Figure 1


Mesenchymal stromal cells for cutaneous wound healing in a rabbit model: pre-clinical study applicable in the pediatric surgical setting.

Pelizzo G, Avanzini MA, Icaro Cornaglia A, Osti M, Romano P, Avolio L, Maccario R, Dominici M, De Silvestri A, Andreatta E, Costanzo F, Mantelli M, Ingo D, Piccinno S, Calcaterra V - J Transl Med (2015)

Experimental design. Rabbit ASCs and BM-MSCs were collected, isolated and expanded. As soon as an adequate number of in vitro expanded MSCs was reached, two wound lesions (wound 1 and wound 2) were created. Within 5 min from the wound lesion establishment, autologous or allogeneic ASCs or autologous BM-MSCs were directly injected into the wound bed 1. Into wound bed 2, 3 ml of saline 2% rabbit albumin solution was injected as a control. Wound healing was monitored daily. After rabbit euthanasia, biopsies of the regenerated tissues were collected, using dermal biopsy punches, for histological examination.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4495634&req=5

Fig1: Experimental design. Rabbit ASCs and BM-MSCs were collected, isolated and expanded. As soon as an adequate number of in vitro expanded MSCs was reached, two wound lesions (wound 1 and wound 2) were created. Within 5 min from the wound lesion establishment, autologous or allogeneic ASCs or autologous BM-MSCs were directly injected into the wound bed 1. Into wound bed 2, 3 ml of saline 2% rabbit albumin solution was injected as a control. Wound healing was monitored daily. After rabbit euthanasia, biopsies of the regenerated tissues were collected, using dermal biopsy punches, for histological examination.
Mentions: For the experimental outline see Figure 1.Figure 1

Bottom Line: Rabbit ASCs were isolated and expanded in vitro with relative abundance, cells expressed typical surface markers (CD49e, CD90 and CD29).Improved re-epithelization, reduced inflammatory infiltration and increased collagen deposition were observed in biopsies from wounds treated with ASCs, with the best result in the autologous setting.Pre-clinical studies are however necessary to validate the best skin regeneration technique, which could be used in pediatric surgical translational research.

View Article: PubMed Central - PubMed

Affiliation: Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo and University of Pavia, 27100, Pavia, Italy. g.pelizzo@smatteo.pv.it.

ABSTRACT

Objective: Mesenchymal stromal cells (MSCs) expanded in vitro have been proposed as a potential therapy for congenital or acquired skin defects in pediatrics. The aim of this pre-clinical study was to investigate the effects of intradermal injections of MSC in experimental cutaneous wound repair comparing allogeneic and autologous adipose stem cells (ASCs) and autologous bone marrow-mesenchymal stromal cells (BM-MSCs).

Methods: Mesenchymal stromal cells were in vitro expanded from adipose and BM tissues of young female New Zealand rabbits. MSCs were characterized for plastic adhesion, surface markers, proliferation and differentiation capacity. When an adequate number of cells (ASCs 10 × 10(6) and BM-MSCs 3 × 10(6), because of their low rate of proliferation) was reached, two skin wounds were surgically induced in each animal. The first was topically treated with cell infusions, the second was used as a control. The intradermal inoculation included autologous or allogeneic ASCs or autologous BM-MSCs. For histological examination, animals were sacrificed and wounds were harvested after 11 and 21 days of treatment.

Results: Rabbit ASCs were isolated and expanded in vitro with relative abundance, cells expressed typical surface markers (CD49e, CD90 and CD29). Topically, ASC inoculation provided more rapid wound healing than BM-MSCs and controls. Improved re-epithelization, reduced inflammatory infiltration and increased collagen deposition were observed in biopsies from wounds treated with ASCs, with the best result in the autologous setting. ASCs also improved restoration of skin architecture during wound healing.

Conclusion: The use of ASCs may offer a promising solution to treat extended wounds. Pre-clinical studies are however necessary to validate the best skin regeneration technique, which could be used in pediatric surgical translational research.

No MeSH data available.


Related in: MedlinePlus