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TOL19-001 reduces inflammation and MMP expression in monolayer cultures of tendon cells.

Baugé C, Leclercq S, Conrozier T, Boumediene K - BMC Complement Altern Med (2015)

Bottom Line: Scleraxis expression was also increased by CIP whereas it was decreased by IL-1β treatment.These findings indicate that TOL19-001 exerts anti-inflammatory effects on tendon cells, which might explain why TOL19-001 diet may improve tendon function in patients with tendon injury.Future research is required to determine TOL19-001 effect on injured or overused tendons in vivo.

View Article: PubMed Central - PubMed

Affiliation: Normandie Univ, Caen, France. catherine.bauge@unicaen.fr.

ABSTRACT

Background: Tendinopathies are tendon conditions associated with degeneration and disorganization of the matrix collagen fibers, tendon cells apoptosis and inflammation through up-regulation of proinflammatory cytokines, matrix metalloproteinase (MMP) expression, and prostaglandin E2 (PGE2) production. Currently, the pharmacological treatment is mainly based on non-steroidal anti-inflammatory drugs (NSAIDs) use and corticosteroid injections, which both can lead to numerous side effects for patients. TOL19-001 is a diet supplementary composed mostly of spirulina and glucosamine sulfate whose antioxidant properties could be helpful to treat tendinopathies while avoiding taking NSAIDs. In this study we developed an in vitro model of tendinopathy in order to evaluate the therapeutic potential of TOL19-001.

Methods: Tendon cells were cultured on monolayer and treated with interleukin-1β (IL-1β) or ciprofloxacin (CIP), and then, MMPs, PGE2 and collagen expression was evaluated by RT-PCR or Elisa. In addition, a cotreatment with increased doses of TOL19-001 was done. Toxicity of TOL19-001 was evaluated using a metabolic activity assay.

Results: This study demonstrates that IL-1β mimics some aspects of tendinopathies with PGE2 induction, MMP expression (mostly MMP1 and MMP3), and increases of type III/I collagen ratio. CIP, meanwhile, leads to an increase of MMP2 and p65 mRNA, whereas it reduces TIMP1 expression. Scleraxis expression was also increased by CIP whereas it was decreased by IL-1β treatment. Besides, TOL19-001 cotreatment suppresses tendon cell inflammation in vitro, marked by the downregulation of PGE2, MMPs and type III collagen in IL-1β stimulated-cells. TOL19-001 also represses CIP induced-changes.

Conclusions: These findings indicate that TOL19-001 exerts anti-inflammatory effects on tendon cells, which might explain why TOL19-001 diet may improve tendon function in patients with tendon injury. Future research is required to determine TOL19-001 effect on injured or overused tendons in vivo.

No MeSH data available.


Related in: MedlinePlus

Effect of CIP and IL-1β on p65 mRNA level and PGE2 release. Tendon cells were treated as Fig. 1, and p65 mRNA level (a) and PGE2 release (b) were determined by RT-PCR and ELISA, respectively. Histograms represent mean values from 3 independent experiments ± SEM
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Fig2: Effect of CIP and IL-1β on p65 mRNA level and PGE2 release. Tendon cells were treated as Fig. 1, and p65 mRNA level (a) and PGE2 release (b) were determined by RT-PCR and ELISA, respectively. Histograms represent mean values from 3 independent experiments ± SEM

Mentions: Since it is known that MMPs are regulated, in part, by NFκB pathway, we hypothesized that IL-1β and CIP may activate this signaling pathway in tendons cells. Insofar as we have previously observed that IL-1β regulates NFκB signaling in chondrocytes, in part, by increasing mRNA expression of the NFκB subunit p65, we postulated that, in tendon cells, IL-1β and CIP may also upregulate p65. Surprisingly, IL-1β (1 ng/ml) did not induce an increased p65 mRNA level in tendon cells after 48 h-treatment (Fig. 2a). However, CIP increased its expression. This suggests that CIP and IL-1β have different mechanisms of action.Fig. 2


TOL19-001 reduces inflammation and MMP expression in monolayer cultures of tendon cells.

Baugé C, Leclercq S, Conrozier T, Boumediene K - BMC Complement Altern Med (2015)

Effect of CIP and IL-1β on p65 mRNA level and PGE2 release. Tendon cells were treated as Fig. 1, and p65 mRNA level (a) and PGE2 release (b) were determined by RT-PCR and ELISA, respectively. Histograms represent mean values from 3 independent experiments ± SEM
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4495633&req=5

Fig2: Effect of CIP and IL-1β on p65 mRNA level and PGE2 release. Tendon cells were treated as Fig. 1, and p65 mRNA level (a) and PGE2 release (b) were determined by RT-PCR and ELISA, respectively. Histograms represent mean values from 3 independent experiments ± SEM
Mentions: Since it is known that MMPs are regulated, in part, by NFκB pathway, we hypothesized that IL-1β and CIP may activate this signaling pathway in tendons cells. Insofar as we have previously observed that IL-1β regulates NFκB signaling in chondrocytes, in part, by increasing mRNA expression of the NFκB subunit p65, we postulated that, in tendon cells, IL-1β and CIP may also upregulate p65. Surprisingly, IL-1β (1 ng/ml) did not induce an increased p65 mRNA level in tendon cells after 48 h-treatment (Fig. 2a). However, CIP increased its expression. This suggests that CIP and IL-1β have different mechanisms of action.Fig. 2

Bottom Line: Scleraxis expression was also increased by CIP whereas it was decreased by IL-1β treatment.These findings indicate that TOL19-001 exerts anti-inflammatory effects on tendon cells, which might explain why TOL19-001 diet may improve tendon function in patients with tendon injury.Future research is required to determine TOL19-001 effect on injured or overused tendons in vivo.

View Article: PubMed Central - PubMed

Affiliation: Normandie Univ, Caen, France. catherine.bauge@unicaen.fr.

ABSTRACT

Background: Tendinopathies are tendon conditions associated with degeneration and disorganization of the matrix collagen fibers, tendon cells apoptosis and inflammation through up-regulation of proinflammatory cytokines, matrix metalloproteinase (MMP) expression, and prostaglandin E2 (PGE2) production. Currently, the pharmacological treatment is mainly based on non-steroidal anti-inflammatory drugs (NSAIDs) use and corticosteroid injections, which both can lead to numerous side effects for patients. TOL19-001 is a diet supplementary composed mostly of spirulina and glucosamine sulfate whose antioxidant properties could be helpful to treat tendinopathies while avoiding taking NSAIDs. In this study we developed an in vitro model of tendinopathy in order to evaluate the therapeutic potential of TOL19-001.

Methods: Tendon cells were cultured on monolayer and treated with interleukin-1β (IL-1β) or ciprofloxacin (CIP), and then, MMPs, PGE2 and collagen expression was evaluated by RT-PCR or Elisa. In addition, a cotreatment with increased doses of TOL19-001 was done. Toxicity of TOL19-001 was evaluated using a metabolic activity assay.

Results: This study demonstrates that IL-1β mimics some aspects of tendinopathies with PGE2 induction, MMP expression (mostly MMP1 and MMP3), and increases of type III/I collagen ratio. CIP, meanwhile, leads to an increase of MMP2 and p65 mRNA, whereas it reduces TIMP1 expression. Scleraxis expression was also increased by CIP whereas it was decreased by IL-1β treatment. Besides, TOL19-001 cotreatment suppresses tendon cell inflammation in vitro, marked by the downregulation of PGE2, MMPs and type III collagen in IL-1β stimulated-cells. TOL19-001 also represses CIP induced-changes.

Conclusions: These findings indicate that TOL19-001 exerts anti-inflammatory effects on tendon cells, which might explain why TOL19-001 diet may improve tendon function in patients with tendon injury. Future research is required to determine TOL19-001 effect on injured or overused tendons in vivo.

No MeSH data available.


Related in: MedlinePlus