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Determining the Prevalence and Seasonality of Fasciola hepatica in Pasture-based Dairy herds in Ireland using a Bulk Tank Milk ELISA.

Bloemhoff Y, Forbes A, Danaher M, Good B, Morgan E, Mulcahy G, Sekiya M, Sayers R - Ir Vet J (2015)

Bottom Line: A within-herd prevalence of ≤ 50 % was found for herds with negative bulk tank milk samples.The seasonal pattern of F. hepatica shows elevated antibodies as the grazing season progressed, reaching a peak in January.A significant association was found between F. hepatica and age at first calving.

View Article: PubMed Central - PubMed

Affiliation: Animal and Bioscience Research Department, Animal & Grassland Research and Innovation Centre, Teagasc, Moorepark, Fermoy, Co. Cork, Republic of Ireland.

ABSTRACT

Background: Fasciola hepatica is a helminth parasite of global importance in livestock, with major economic impact. However information on F. hepatica infections in Irish pasture-based dairy herds is limited. Therefore this study was conducted in order to determine the prevalence, seasonality and management factors associated with F. hepatica. A total of 319 Irish dairy herds were selected for this study. Bulk tank milk (BTM) samples were collected from 290 dairy farms on a quarter year basis, while from a further 29 dairy farms BTM samples were collected on a monthly basis to provide a more detailed pattern of F. hepatica exposure in Irish herds. BTM samples were analysed using a commercially available F. hepatica antibody detection ELISA. Furthermore, within-herd prevalence of F. hepatica was assessed in a subset of these 29 herds (n = 17); both individual serum samples and bulk tank milk samples were collected.

Results: A within-herd prevalence of ≤ 50 % was found for herds with negative bulk tank milk samples. The mean prevalence of the 290 study herds was 75.4 % (Range 52 %-75.1 %), with the highest prevalence being observed in November (75.1 %). The seasonal pattern of F. hepatica shows elevated antibodies as the grazing season progressed, reaching a peak in January. A significant association was found between F. hepatica and age at first calving.

Conclusion: This study demonstrates that F. hepatica is present in a large proportion of Irish dairy herds and provides a basis on which control practices, particularly in adult dairy cows, can be reviewed.

No MeSH data available.


Related in: MedlinePlus

The within-prevalence of ‘Dairy17’ herds showing the correlation between positive individual blood S/P values and corresponding mean positive BTM herd samples
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Fig2: The within-prevalence of ‘Dairy17’ herds showing the correlation between positive individual blood S/P values and corresponding mean positive BTM herd samples

Mentions: The ‘Dairy17’ herds were used to evaluate the relationship between the proportion of positive blood samples and the matching bulk tank milk S/P value (Fig. 2). In addition the individual blood samples of each herd were plotted in a boxplot, with attached bulk tank milk sample S/P result (Fig. 3). Each of the herds was categorized relative to the bulk tank milk result (Negative/None, low positive, moderate positive and high positive). The within-herd prevalence of F. hepatica of the ‘Dairy17’ herds was calculated as the number of individual cows positive at the >20 S/P value as a percentage of all cows serum sampled. Both Figs. 2 and 3 show a good agreement between the BTM S/P range and the percentage of cows positive to F. hepatica. The three herds that had a negative BTM result had less than half of the cows’ serum samples positive to F. hepatica. In addition these negative BTM herds had considerably lower mean S/P values (S/P = 21), compared to positive BTM herds (S/P = 73). There was a good correlation between BTM S/P results and the individual blood S/P results in the subset of BTM positive herds (Fig. 3). BTM positive herds had a mean within-herd prevalence of approximately 88 % of cows seropositive to F. hepatica.


Determining the Prevalence and Seasonality of Fasciola hepatica in Pasture-based Dairy herds in Ireland using a Bulk Tank Milk ELISA.

Bloemhoff Y, Forbes A, Danaher M, Good B, Morgan E, Mulcahy G, Sekiya M, Sayers R - Ir Vet J (2015)

The within-prevalence of ‘Dairy17’ herds showing the correlation between positive individual blood S/P values and corresponding mean positive BTM herd samples
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4495626&req=5

Fig2: The within-prevalence of ‘Dairy17’ herds showing the correlation between positive individual blood S/P values and corresponding mean positive BTM herd samples
Mentions: The ‘Dairy17’ herds were used to evaluate the relationship between the proportion of positive blood samples and the matching bulk tank milk S/P value (Fig. 2). In addition the individual blood samples of each herd were plotted in a boxplot, with attached bulk tank milk sample S/P result (Fig. 3). Each of the herds was categorized relative to the bulk tank milk result (Negative/None, low positive, moderate positive and high positive). The within-herd prevalence of F. hepatica of the ‘Dairy17’ herds was calculated as the number of individual cows positive at the >20 S/P value as a percentage of all cows serum sampled. Both Figs. 2 and 3 show a good agreement between the BTM S/P range and the percentage of cows positive to F. hepatica. The three herds that had a negative BTM result had less than half of the cows’ serum samples positive to F. hepatica. In addition these negative BTM herds had considerably lower mean S/P values (S/P = 21), compared to positive BTM herds (S/P = 73). There was a good correlation between BTM S/P results and the individual blood S/P results in the subset of BTM positive herds (Fig. 3). BTM positive herds had a mean within-herd prevalence of approximately 88 % of cows seropositive to F. hepatica.

Bottom Line: A within-herd prevalence of ≤ 50 % was found for herds with negative bulk tank milk samples.The seasonal pattern of F. hepatica shows elevated antibodies as the grazing season progressed, reaching a peak in January.A significant association was found between F. hepatica and age at first calving.

View Article: PubMed Central - PubMed

Affiliation: Animal and Bioscience Research Department, Animal & Grassland Research and Innovation Centre, Teagasc, Moorepark, Fermoy, Co. Cork, Republic of Ireland.

ABSTRACT

Background: Fasciola hepatica is a helminth parasite of global importance in livestock, with major economic impact. However information on F. hepatica infections in Irish pasture-based dairy herds is limited. Therefore this study was conducted in order to determine the prevalence, seasonality and management factors associated with F. hepatica. A total of 319 Irish dairy herds were selected for this study. Bulk tank milk (BTM) samples were collected from 290 dairy farms on a quarter year basis, while from a further 29 dairy farms BTM samples were collected on a monthly basis to provide a more detailed pattern of F. hepatica exposure in Irish herds. BTM samples were analysed using a commercially available F. hepatica antibody detection ELISA. Furthermore, within-herd prevalence of F. hepatica was assessed in a subset of these 29 herds (n = 17); both individual serum samples and bulk tank milk samples were collected.

Results: A within-herd prevalence of ≤ 50 % was found for herds with negative bulk tank milk samples. The mean prevalence of the 290 study herds was 75.4 % (Range 52 %-75.1 %), with the highest prevalence being observed in November (75.1 %). The seasonal pattern of F. hepatica shows elevated antibodies as the grazing season progressed, reaching a peak in January. A significant association was found between F. hepatica and age at first calving.

Conclusion: This study demonstrates that F. hepatica is present in a large proportion of Irish dairy herds and provides a basis on which control practices, particularly in adult dairy cows, can be reviewed.

No MeSH data available.


Related in: MedlinePlus