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Discrimination of Transgenic Rice containing the Cry1Ab Protein using Terahertz Spectroscopy and Chemometrics.

Xu W, Xie L, Ye Z, Gao W, Yao Y, Chen M, Qin J, Ying Y - Sci Rep (2015)

Bottom Line: In this work, terahertz time-domain spectroscopy (THz-TDS) combined with discriminate analysis (DA) and principal component analysis (PCA) with derivative pretreatments was performed to differentiate transgenic rice (Hua Hui 1, containing the Cry1Ab protein) from its parent (Ming Hui 63).Both rice samples and the Cry1Ab protein were ground and pressed into pellets for terahertz (THz) measurements.By applying the first derivative of the THz spectra in conjunction with the DA model, the discrimination of transgenic from non-transgenic rice was possible with accuracies up to 89.4% and 85.0% for the calibration set and validation set, respectively.

View Article: PubMed Central - PubMed

Affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Rd., 310058 Hangzhou, PR China.

ABSTRACT
Spectroscopic techniques combined with chemometrics methods have proven to be effective tools for the discrimination of objects with similar properties. In this work, terahertz time-domain spectroscopy (THz-TDS) combined with discriminate analysis (DA) and principal component analysis (PCA) with derivative pretreatments was performed to differentiate transgenic rice (Hua Hui 1, containing the Cry1Ab protein) from its parent (Ming Hui 63). Both rice samples and the Cry1Ab protein were ground and pressed into pellets for terahertz (THz) measurements. The resulting time-domain spectra were transformed into frequency-domain spectra, and then, the transmittances of the rice and Cry1Ab protein were calculated. By applying the first derivative of the THz spectra in conjunction with the DA model, the discrimination of transgenic from non-transgenic rice was possible with accuracies up to 89.4% and 85.0% for the calibration set and validation set, respectively. The results indicated that THz spectroscopic techniques and chemometrics methods could be new feasible ways to differentiate transgenic rice.

No MeSH data available.


Related in: MedlinePlus

Discrimination results for the transgenic and non-transgenic rice samples using the DA method and the THz spectra with the first derivate pretreatment.
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Related In: Results  -  Collection

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f4: Discrimination results for the transgenic and non-transgenic rice samples using the DA method and the THz spectra with the first derivate pretreatment.

Mentions: Figure 4 shows a plot of the Mahalanobis distance of the THz spectroscopy of the rice samples with the first derivative pretreatment. This plot shows transgenic rice and non-transgenic rice divided into two groups with a few samples overlapping each other. Therefore, the DA method demonstrates good capability for differentiating the rice with different genotypes.


Discrimination of Transgenic Rice containing the Cry1Ab Protein using Terahertz Spectroscopy and Chemometrics.

Xu W, Xie L, Ye Z, Gao W, Yao Y, Chen M, Qin J, Ying Y - Sci Rep (2015)

Discrimination results for the transgenic and non-transgenic rice samples using the DA method and the THz spectra with the first derivate pretreatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4495602&req=5

f4: Discrimination results for the transgenic and non-transgenic rice samples using the DA method and the THz spectra with the first derivate pretreatment.
Mentions: Figure 4 shows a plot of the Mahalanobis distance of the THz spectroscopy of the rice samples with the first derivative pretreatment. This plot shows transgenic rice and non-transgenic rice divided into two groups with a few samples overlapping each other. Therefore, the DA method demonstrates good capability for differentiating the rice with different genotypes.

Bottom Line: In this work, terahertz time-domain spectroscopy (THz-TDS) combined with discriminate analysis (DA) and principal component analysis (PCA) with derivative pretreatments was performed to differentiate transgenic rice (Hua Hui 1, containing the Cry1Ab protein) from its parent (Ming Hui 63).Both rice samples and the Cry1Ab protein were ground and pressed into pellets for terahertz (THz) measurements.By applying the first derivative of the THz spectra in conjunction with the DA model, the discrimination of transgenic from non-transgenic rice was possible with accuracies up to 89.4% and 85.0% for the calibration set and validation set, respectively.

View Article: PubMed Central - PubMed

Affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Rd., 310058 Hangzhou, PR China.

ABSTRACT
Spectroscopic techniques combined with chemometrics methods have proven to be effective tools for the discrimination of objects with similar properties. In this work, terahertz time-domain spectroscopy (THz-TDS) combined with discriminate analysis (DA) and principal component analysis (PCA) with derivative pretreatments was performed to differentiate transgenic rice (Hua Hui 1, containing the Cry1Ab protein) from its parent (Ming Hui 63). Both rice samples and the Cry1Ab protein were ground and pressed into pellets for terahertz (THz) measurements. The resulting time-domain spectra were transformed into frequency-domain spectra, and then, the transmittances of the rice and Cry1Ab protein were calculated. By applying the first derivative of the THz spectra in conjunction with the DA model, the discrimination of transgenic from non-transgenic rice was possible with accuracies up to 89.4% and 85.0% for the calibration set and validation set, respectively. The results indicated that THz spectroscopic techniques and chemometrics methods could be new feasible ways to differentiate transgenic rice.

No MeSH data available.


Related in: MedlinePlus