Limits...
Discrimination of Transgenic Rice containing the Cry1Ab Protein using Terahertz Spectroscopy and Chemometrics.

Xu W, Xie L, Ye Z, Gao W, Yao Y, Chen M, Qin J, Ying Y - Sci Rep (2015)

Bottom Line: In this work, terahertz time-domain spectroscopy (THz-TDS) combined with discriminate analysis (DA) and principal component analysis (PCA) with derivative pretreatments was performed to differentiate transgenic rice (Hua Hui 1, containing the Cry1Ab protein) from its parent (Ming Hui 63).Both rice samples and the Cry1Ab protein were ground and pressed into pellets for terahertz (THz) measurements.By applying the first derivative of the THz spectra in conjunction with the DA model, the discrimination of transgenic from non-transgenic rice was possible with accuracies up to 89.4% and 85.0% for the calibration set and validation set, respectively.

View Article: PubMed Central - PubMed

Affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Rd., 310058 Hangzhou, PR China.

ABSTRACT
Spectroscopic techniques combined with chemometrics methods have proven to be effective tools for the discrimination of objects with similar properties. In this work, terahertz time-domain spectroscopy (THz-TDS) combined with discriminate analysis (DA) and principal component analysis (PCA) with derivative pretreatments was performed to differentiate transgenic rice (Hua Hui 1, containing the Cry1Ab protein) from its parent (Ming Hui 63). Both rice samples and the Cry1Ab protein were ground and pressed into pellets for terahertz (THz) measurements. The resulting time-domain spectra were transformed into frequency-domain spectra, and then, the transmittances of the rice and Cry1Ab protein were calculated. By applying the first derivative of the THz spectra in conjunction with the DA model, the discrimination of transgenic from non-transgenic rice was possible with accuracies up to 89.4% and 85.0% for the calibration set and validation set, respectively. The results indicated that THz spectroscopic techniques and chemometrics methods could be new feasible ways to differentiate transgenic rice.

No MeSH data available.


Related in: MedlinePlus

Three-dimensional score plot of the first three principle components for the transgenic and non-transgenic rice samples.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4495602&req=5

f3: Three-dimensional score plot of the first three principle components for the transgenic and non-transgenic rice samples.

Mentions: where T(0%) is the transmittance of the PE pellet and T(sample) is the transmittance of a Cry1Ab pellet sample. Strong peaks at 1.20, 1.40, 1.70, 2.05 and 2.20 THz appeared, as shown in the difference transmittance spectra in Fig. 3c. Because L-glutamic acid shows absorption peaks at 1.20 and 2.05 THz, L-cysteine shows absorption peaks at 1.40 and 1.70 THz, and L-histidine shows an absorption peak at 2.20 THz27, these peaks may be assigned to amino acids. Because the Cry1Ab protein is mainly composed of different types of amino acids, these characteristic peaks of the Cry1Ab protein may be caused by its amino acids. These spectra overlapped each other, and no clear correlation between the difference transmittance and the concentration was shown, except for the 1.30–1.60 THz and 1.75–2.00 THz bands. The inset of Fig. 2c shows the transmittance spectra of the Cry1Ab pellet samples in the range of 1.75–2.00 THz. In the region of 1.75–2.00 THz, the waveforms of the difference transmittance spectra could be distinguished from each other, and the amplitude increased with the concentration of the Cry1Ab protein. That is, the difference transmittance shape changed with the Cry1Ab protein concentration and the PE mixing ratio and could be separated in the 1.30–1.60 THz and 1.75–2.00 THz bands.


Discrimination of Transgenic Rice containing the Cry1Ab Protein using Terahertz Spectroscopy and Chemometrics.

Xu W, Xie L, Ye Z, Gao W, Yao Y, Chen M, Qin J, Ying Y - Sci Rep (2015)

Three-dimensional score plot of the first three principle components for the transgenic and non-transgenic rice samples.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4495602&req=5

f3: Three-dimensional score plot of the first three principle components for the transgenic and non-transgenic rice samples.
Mentions: where T(0%) is the transmittance of the PE pellet and T(sample) is the transmittance of a Cry1Ab pellet sample. Strong peaks at 1.20, 1.40, 1.70, 2.05 and 2.20 THz appeared, as shown in the difference transmittance spectra in Fig. 3c. Because L-glutamic acid shows absorption peaks at 1.20 and 2.05 THz, L-cysteine shows absorption peaks at 1.40 and 1.70 THz, and L-histidine shows an absorption peak at 2.20 THz27, these peaks may be assigned to amino acids. Because the Cry1Ab protein is mainly composed of different types of amino acids, these characteristic peaks of the Cry1Ab protein may be caused by its amino acids. These spectra overlapped each other, and no clear correlation between the difference transmittance and the concentration was shown, except for the 1.30–1.60 THz and 1.75–2.00 THz bands. The inset of Fig. 2c shows the transmittance spectra of the Cry1Ab pellet samples in the range of 1.75–2.00 THz. In the region of 1.75–2.00 THz, the waveforms of the difference transmittance spectra could be distinguished from each other, and the amplitude increased with the concentration of the Cry1Ab protein. That is, the difference transmittance shape changed with the Cry1Ab protein concentration and the PE mixing ratio and could be separated in the 1.30–1.60 THz and 1.75–2.00 THz bands.

Bottom Line: In this work, terahertz time-domain spectroscopy (THz-TDS) combined with discriminate analysis (DA) and principal component analysis (PCA) with derivative pretreatments was performed to differentiate transgenic rice (Hua Hui 1, containing the Cry1Ab protein) from its parent (Ming Hui 63).Both rice samples and the Cry1Ab protein were ground and pressed into pellets for terahertz (THz) measurements.By applying the first derivative of the THz spectra in conjunction with the DA model, the discrimination of transgenic from non-transgenic rice was possible with accuracies up to 89.4% and 85.0% for the calibration set and validation set, respectively.

View Article: PubMed Central - PubMed

Affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Rd., 310058 Hangzhou, PR China.

ABSTRACT
Spectroscopic techniques combined with chemometrics methods have proven to be effective tools for the discrimination of objects with similar properties. In this work, terahertz time-domain spectroscopy (THz-TDS) combined with discriminate analysis (DA) and principal component analysis (PCA) with derivative pretreatments was performed to differentiate transgenic rice (Hua Hui 1, containing the Cry1Ab protein) from its parent (Ming Hui 63). Both rice samples and the Cry1Ab protein were ground and pressed into pellets for terahertz (THz) measurements. The resulting time-domain spectra were transformed into frequency-domain spectra, and then, the transmittances of the rice and Cry1Ab protein were calculated. By applying the first derivative of the THz spectra in conjunction with the DA model, the discrimination of transgenic from non-transgenic rice was possible with accuracies up to 89.4% and 85.0% for the calibration set and validation set, respectively. The results indicated that THz spectroscopic techniques and chemometrics methods could be new feasible ways to differentiate transgenic rice.

No MeSH data available.


Related in: MedlinePlus