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Creating Anti-icing Surfaces via the Direct Immobilization of Antifreeze Proteins on Aluminum.

Gwak Y, Park JI, Kim M, Kim HS, Kwon MJ, Oh SJ, Kim YP, Jin E - Sci Rep (2015)

Bottom Line: The ABP bound well to the Al and did not considerably change the functional properties of AFP.Additional trehalose coating of Cn-AFP-Al considerably delayed AFP denaturation on the Al without affecting its antifreeze activity.This metal surface-coating method using trehalose-fortified AFP can be applied to other metals important in the aircraft and cold storage fields where anti-icing materials are critical.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Research Institute for Natural Science, Hanyang University, Seoul, 133-791, South Korea.

ABSTRACT
Cryoprotectants such as antifreeze proteins (AFPs) and sugar molecules may provide a solution for icing problems. These anti-icing substances protect cells and tissues from freezing by inhibiting ice formation. In this study, we developed a method for coating an industrial metal material (aluminum, Al) with AFP from the Antarctic marine diatom, Chaetoceros neogracile (Cn-AFP), to prevent or delay ice formation. To coat Al with Cn-AFP, we used an Al-binding peptide (ABP) as a conjugator and fused it with Cn-AFP. The ABP bound well to the Al and did not considerably change the functional properties of AFP. Cn-AFP-coated Al (Cn-AFP-Al) showed a sufficiently low supercooling point. Additional trehalose coating of Cn-AFP-Al considerably delayed AFP denaturation on the Al without affecting its antifreeze activity. This metal surface-coating method using trehalose-fortified AFP can be applied to other metals important in the aircraft and cold storage fields where anti-icing materials are critical.

No MeSH data available.


The anti-icing effect of Tre-ABP-Cn-AFPG124Y on Al.The ABP-Cn-AFPG124Y coating fortified by trehalose inhibited ice crystal growth and impeded frost formation, while large amounts of ice and frost accumulated on bare Al and on Al with a hydrophilic ZrO2 coating.
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f7: The anti-icing effect of Tre-ABP-Cn-AFPG124Y on Al.The ABP-Cn-AFPG124Y coating fortified by trehalose inhibited ice crystal growth and impeded frost formation, while large amounts of ice and frost accumulated on bare Al and on Al with a hydrophilic ZrO2 coating.

Mentions: To investigate the effect of trehalose on the anti-icing activity of ABP-Cn-AFP, the supercooling point of ABP-Cn-AFPG124Y was measured via differential scanning calorimetry both with and without trehalose treatment of the Al surface. Figure 6 shows a representative thermogram of the ABP-Cn-AFPG124Y with and without trehalose on Al cooled at 0.1 °C min−1 from 0 °C to −25 °C. The supercooling point of ABP-Cn-AFPG124Y in the presence of trehalose (−7.92 °C, Fig. 6A) is similar to that in the absence of trehalose (−8.43 °C, Fig. 6B). This result indicates that trehalose treatment did not affect the anti-icing activity of ABP-Cn-AFPG124Y. To further explore the possibility that directly immobilized AFP on Al prevents ice or frost formation, we examined time-dependent ice formation in a cold chamber for 3 h. Notably, Cn-AFP-immobilized on the Al surface (ABP-Cn-AFPG124Y with trehalose) prevented ice formation, whereas ice formed on bare Al and hydrophilic Al surfaces coated with a thin ZrO2 film, which is a universal surface-coating method for Al2627282930 (Fig. 7). This result demonstrates that direct immobilization of ABP-CnAFPG124Y followed by trehalose treatment inhibits ice formation on an Al surface.


Creating Anti-icing Surfaces via the Direct Immobilization of Antifreeze Proteins on Aluminum.

Gwak Y, Park JI, Kim M, Kim HS, Kwon MJ, Oh SJ, Kim YP, Jin E - Sci Rep (2015)

The anti-icing effect of Tre-ABP-Cn-AFPG124Y on Al.The ABP-Cn-AFPG124Y coating fortified by trehalose inhibited ice crystal growth and impeded frost formation, while large amounts of ice and frost accumulated on bare Al and on Al with a hydrophilic ZrO2 coating.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4495550&req=5

f7: The anti-icing effect of Tre-ABP-Cn-AFPG124Y on Al.The ABP-Cn-AFPG124Y coating fortified by trehalose inhibited ice crystal growth and impeded frost formation, while large amounts of ice and frost accumulated on bare Al and on Al with a hydrophilic ZrO2 coating.
Mentions: To investigate the effect of trehalose on the anti-icing activity of ABP-Cn-AFP, the supercooling point of ABP-Cn-AFPG124Y was measured via differential scanning calorimetry both with and without trehalose treatment of the Al surface. Figure 6 shows a representative thermogram of the ABP-Cn-AFPG124Y with and without trehalose on Al cooled at 0.1 °C min−1 from 0 °C to −25 °C. The supercooling point of ABP-Cn-AFPG124Y in the presence of trehalose (−7.92 °C, Fig. 6A) is similar to that in the absence of trehalose (−8.43 °C, Fig. 6B). This result indicates that trehalose treatment did not affect the anti-icing activity of ABP-Cn-AFPG124Y. To further explore the possibility that directly immobilized AFP on Al prevents ice or frost formation, we examined time-dependent ice formation in a cold chamber for 3 h. Notably, Cn-AFP-immobilized on the Al surface (ABP-Cn-AFPG124Y with trehalose) prevented ice formation, whereas ice formed on bare Al and hydrophilic Al surfaces coated with a thin ZrO2 film, which is a universal surface-coating method for Al2627282930 (Fig. 7). This result demonstrates that direct immobilization of ABP-CnAFPG124Y followed by trehalose treatment inhibits ice formation on an Al surface.

Bottom Line: The ABP bound well to the Al and did not considerably change the functional properties of AFP.Additional trehalose coating of Cn-AFP-Al considerably delayed AFP denaturation on the Al without affecting its antifreeze activity.This metal surface-coating method using trehalose-fortified AFP can be applied to other metals important in the aircraft and cold storage fields where anti-icing materials are critical.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Research Institute for Natural Science, Hanyang University, Seoul, 133-791, South Korea.

ABSTRACT
Cryoprotectants such as antifreeze proteins (AFPs) and sugar molecules may provide a solution for icing problems. These anti-icing substances protect cells and tissues from freezing by inhibiting ice formation. In this study, we developed a method for coating an industrial metal material (aluminum, Al) with AFP from the Antarctic marine diatom, Chaetoceros neogracile (Cn-AFP), to prevent or delay ice formation. To coat Al with Cn-AFP, we used an Al-binding peptide (ABP) as a conjugator and fused it with Cn-AFP. The ABP bound well to the Al and did not considerably change the functional properties of AFP. Cn-AFP-coated Al (Cn-AFP-Al) showed a sufficiently low supercooling point. Additional trehalose coating of Cn-AFP-Al considerably delayed AFP denaturation on the Al without affecting its antifreeze activity. This metal surface-coating method using trehalose-fortified AFP can be applied to other metals important in the aircraft and cold storage fields where anti-icing materials are critical.

No MeSH data available.