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Pathogenic diversity amongst serotype C VGIII and VGIV Cryptococcus gattii isolates.

Rodrigues J, Fonseca FL, Schneider RO, Godinho RM, Firacative C, Maszewska K, Meyer W, Schrank A, Staats C, Kmetzsch L, Vainstein MH, Rodrigues ML - Sci Rep (2015)

Bottom Line: This great phenotypic diversity reflected in differential pathogenicity.VGIV isolates WM 779 and 106.97 were similar in their ability to cause lethality and produced higher pulmonary fungal burden in a murine model of cryptococcosis, while isolate ATCC 24066 (VGIII) was unable to reach the brain and caused reduced lethality in intranasally infected mice.These results demonstrate a high diversity in the pathogenic potential of isolates of C. gattii belonging to the molecular types VGIII and VGIV.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Microbiologia Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

ABSTRACT
Cryptococcus gattii is one of the causative agents of human cryptococcosis. Highly virulent strains of serotype B C. gattii have been studied in detail, but little information is available on the pathogenic properties of serotype C isolates. In this study, we analyzed pathogenic determinants in three serotype C C. gattii isolates (106.97, ATCC 24066 and WM 779). Isolate ATCC 24066 (molecular type VGIII) differed from isolates WM 779 and 106.97 (both VGIV) in capsule dimensions, expression of CAP genes, chitooligomer distribution, and induction of host chitinase activity. Isolate WM 779 was more efficient than the others in producing pigments and all three isolates had distinct patterns of reactivity with antibodies to glucuronoxylomannan. This great phenotypic diversity reflected in differential pathogenicity. VGIV isolates WM 779 and 106.97 were similar in their ability to cause lethality and produced higher pulmonary fungal burden in a murine model of cryptococcosis, while isolate ATCC 24066 (VGIII) was unable to reach the brain and caused reduced lethality in intranasally infected mice. These results demonstrate a high diversity in the pathogenic potential of isolates of C. gattii belonging to the molecular types VGIII and VGIV.

No MeSH data available.


Related in: MedlinePlus

Glycosyl composition of polysaccharide extracts from the C. gattii isolates analyzed in this study.Monosaccharides were obtained after methanolysis of polysaccharide extracts for further GC-MS analysis, as detailed in the Methods section.
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f4: Glycosyl composition of polysaccharide extracts from the C. gattii isolates analyzed in this study.Monosaccharides were obtained after methanolysis of polysaccharide extracts for further GC-MS analysis, as detailed in the Methods section.

Mentions: Capsular polysaccharides were extracted with DMSO and the resulting fractions had their glycosidic composition determined by GC-MS (Fig. 4). All isolates had similar xylose : glucuronic acid : mannose ratios, which was in accordance with previous studies determining the composition of capsular extracts from isolates belonging to VGIII and VGIV molecular types (serotype C)35. Other sugar units, however, varied in each isolate. Galactose, which was detected in trace amounts in capsular samples from isolate ATCC 24066, was abundant in fractions obtained from isolates 106.97 and WM 779. Glucose was highly concentrated in the samples obtained from isolates ATCC 24066 and 106.97, but not in the glycan fraction extracted from isolate WM 779. Finally, N-acetylglucosamine, which was absent in ATCC 24066 and WM 779 samples, was present at the trace level in fractions extracted from isolate 106.97.


Pathogenic diversity amongst serotype C VGIII and VGIV Cryptococcus gattii isolates.

Rodrigues J, Fonseca FL, Schneider RO, Godinho RM, Firacative C, Maszewska K, Meyer W, Schrank A, Staats C, Kmetzsch L, Vainstein MH, Rodrigues ML - Sci Rep (2015)

Glycosyl composition of polysaccharide extracts from the C. gattii isolates analyzed in this study.Monosaccharides were obtained after methanolysis of polysaccharide extracts for further GC-MS analysis, as detailed in the Methods section.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4495446&req=5

f4: Glycosyl composition of polysaccharide extracts from the C. gattii isolates analyzed in this study.Monosaccharides were obtained after methanolysis of polysaccharide extracts for further GC-MS analysis, as detailed in the Methods section.
Mentions: Capsular polysaccharides were extracted with DMSO and the resulting fractions had their glycosidic composition determined by GC-MS (Fig. 4). All isolates had similar xylose : glucuronic acid : mannose ratios, which was in accordance with previous studies determining the composition of capsular extracts from isolates belonging to VGIII and VGIV molecular types (serotype C)35. Other sugar units, however, varied in each isolate. Galactose, which was detected in trace amounts in capsular samples from isolate ATCC 24066, was abundant in fractions obtained from isolates 106.97 and WM 779. Glucose was highly concentrated in the samples obtained from isolates ATCC 24066 and 106.97, but not in the glycan fraction extracted from isolate WM 779. Finally, N-acetylglucosamine, which was absent in ATCC 24066 and WM 779 samples, was present at the trace level in fractions extracted from isolate 106.97.

Bottom Line: This great phenotypic diversity reflected in differential pathogenicity.VGIV isolates WM 779 and 106.97 were similar in their ability to cause lethality and produced higher pulmonary fungal burden in a murine model of cryptococcosis, while isolate ATCC 24066 (VGIII) was unable to reach the brain and caused reduced lethality in intranasally infected mice.These results demonstrate a high diversity in the pathogenic potential of isolates of C. gattii belonging to the molecular types VGIII and VGIV.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Microbiologia Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

ABSTRACT
Cryptococcus gattii is one of the causative agents of human cryptococcosis. Highly virulent strains of serotype B C. gattii have been studied in detail, but little information is available on the pathogenic properties of serotype C isolates. In this study, we analyzed pathogenic determinants in three serotype C C. gattii isolates (106.97, ATCC 24066 and WM 779). Isolate ATCC 24066 (molecular type VGIII) differed from isolates WM 779 and 106.97 (both VGIV) in capsule dimensions, expression of CAP genes, chitooligomer distribution, and induction of host chitinase activity. Isolate WM 779 was more efficient than the others in producing pigments and all three isolates had distinct patterns of reactivity with antibodies to glucuronoxylomannan. This great phenotypic diversity reflected in differential pathogenicity. VGIV isolates WM 779 and 106.97 were similar in their ability to cause lethality and produced higher pulmonary fungal burden in a murine model of cryptococcosis, while isolate ATCC 24066 (VGIII) was unable to reach the brain and caused reduced lethality in intranasally infected mice. These results demonstrate a high diversity in the pathogenic potential of isolates of C. gattii belonging to the molecular types VGIII and VGIV.

No MeSH data available.


Related in: MedlinePlus