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Different distribution of Helicobacter pylori EPIYA- cagA motifs and dupA genes in the upper gastrointestinal diseases and correlation with clinical outcomes in iranian patients.

Haddadi MH, Bazargani A, Khashei R, Fattahi MR, Bagheri Lankarani K, Moini M, Rokni Hosseini SM - Gastroenterol Hepatol Bed Bench (2015)

Bottom Line: Of 120 H. pylori isolates, 35.9% were dupA positive and 56.26% were cagA positive, while cagA with ABC and ABCC motifs were 55.5% and 44.5%, respectively.Fifty six percent of the isolates with the ABCC motif have had dupA genes.We also found a significant association between strains with genotypes of dupA-ABC and duodenal ulcer disease (p = 0.007).

View Article: PubMed Central - PubMed

Affiliation: Department of Bacteriology and Virology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Aim: Our aim was to determine the EPIYA-cagA Phosphorylation sites and dupA gene in H. pylori isolates among patients with upper gastrointestinal diseases.

Background: Pathogenicity of the cagA-positive Helicobacter pylori is associated with EPIYA motifs and higher number of EPIYA-C segments is a risk factor of gastric cancer, while duodenal ulcer-promoting gene (dupA) is determined as a protective factor against gastric cancer.

Patients and methods: A total of 280 non-repeated gastric biopsies obtained from patients undergoing endoscopy from January 2013 till July 2013. Samples were cultured on selective horse blood agar and incubated in microaerophilic atmosphere. The isolated organisms were identified as H. pylori by Gram staining and positive oxidase, catalase, and urease tests. Various motif types of cagA and the prevalence of dupA were determined by PCR method.

Results: Out of 280 specimens, 128 (54.7%) isolated organisms were identified as H. pylori. Of 120 H. pylori isolates, 35.9% were dupA positive and 56.26% were cagA positive, while cagA with ABC and ABCC motifs were 55.5% and 44.5%, respectively. Fifty six percent of the isolates with the ABCC motif have had dupA genes. We also found a significant association between strains with genotypes of dupA-ABC and duodenal ulcer disease (p = 0.007).

Conclusion: The results of this study showed that the prevalence of cagA-positive H. pylori in Shiraz was as high as in western countries and higher numbers of EPIYA-C segments were seen in gastric cancer patients. We may also use dupA as a prognostic and pathogenic marker for duodenal ulcer disease and cagA with the segment C for gastric cancer and gastric ulcer disease in this region.

No MeSH data available.


Related in: MedlinePlus

Characterized of cagA EPIYA motifs, ureC and dupA genes. A) ureC with 300 bp. B); Motif ABC, (1): motif A (180 bp), (2): 237 bp motif B (237 bp), (3): motif C (367 bp). C) Motif ABCC: motif A (180 bp), (2): 237 bp motif B (237 bp), (3): motif C (367 bp) and motif CC (480 bp). D) (1) and (2) are positive a sample of gene dupA with 1080 bp. (3): Patient with negative results. Lane (M) DNA ladder from 100-1500 bp.
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Figure 1: Characterized of cagA EPIYA motifs, ureC and dupA genes. A) ureC with 300 bp. B); Motif ABC, (1): motif A (180 bp), (2): 237 bp motif B (237 bp), (3): motif C (367 bp). C) Motif ABCC: motif A (180 bp), (2): 237 bp motif B (237 bp), (3): motif C (367 bp) and motif CC (480 bp). D) (1) and (2) are positive a sample of gene dupA with 1080 bp. (3): Patient with negative results. Lane (M) DNA ladder from 100-1500 bp.

Mentions: Table 2 shows the Primers used in this study. For identification and verification of H. pylori presence at the species level, the ureC gene was amplified using primers glmM, with a 296-bp size product for all samples (Fig 1.A) (12).


Different distribution of Helicobacter pylori EPIYA- cagA motifs and dupA genes in the upper gastrointestinal diseases and correlation with clinical outcomes in iranian patients.

Haddadi MH, Bazargani A, Khashei R, Fattahi MR, Bagheri Lankarani K, Moini M, Rokni Hosseini SM - Gastroenterol Hepatol Bed Bench (2015)

Characterized of cagA EPIYA motifs, ureC and dupA genes. A) ureC with 300 bp. B); Motif ABC, (1): motif A (180 bp), (2): 237 bp motif B (237 bp), (3): motif C (367 bp). C) Motif ABCC: motif A (180 bp), (2): 237 bp motif B (237 bp), (3): motif C (367 bp) and motif CC (480 bp). D) (1) and (2) are positive a sample of gene dupA with 1080 bp. (3): Patient with negative results. Lane (M) DNA ladder from 100-1500 bp.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4495425&req=5

Figure 1: Characterized of cagA EPIYA motifs, ureC and dupA genes. A) ureC with 300 bp. B); Motif ABC, (1): motif A (180 bp), (2): 237 bp motif B (237 bp), (3): motif C (367 bp). C) Motif ABCC: motif A (180 bp), (2): 237 bp motif B (237 bp), (3): motif C (367 bp) and motif CC (480 bp). D) (1) and (2) are positive a sample of gene dupA with 1080 bp. (3): Patient with negative results. Lane (M) DNA ladder from 100-1500 bp.
Mentions: Table 2 shows the Primers used in this study. For identification and verification of H. pylori presence at the species level, the ureC gene was amplified using primers glmM, with a 296-bp size product for all samples (Fig 1.A) (12).

Bottom Line: Of 120 H. pylori isolates, 35.9% were dupA positive and 56.26% were cagA positive, while cagA with ABC and ABCC motifs were 55.5% and 44.5%, respectively.Fifty six percent of the isolates with the ABCC motif have had dupA genes.We also found a significant association between strains with genotypes of dupA-ABC and duodenal ulcer disease (p = 0.007).

View Article: PubMed Central - PubMed

Affiliation: Department of Bacteriology and Virology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Aim: Our aim was to determine the EPIYA-cagA Phosphorylation sites and dupA gene in H. pylori isolates among patients with upper gastrointestinal diseases.

Background: Pathogenicity of the cagA-positive Helicobacter pylori is associated with EPIYA motifs and higher number of EPIYA-C segments is a risk factor of gastric cancer, while duodenal ulcer-promoting gene (dupA) is determined as a protective factor against gastric cancer.

Patients and methods: A total of 280 non-repeated gastric biopsies obtained from patients undergoing endoscopy from January 2013 till July 2013. Samples were cultured on selective horse blood agar and incubated in microaerophilic atmosphere. The isolated organisms were identified as H. pylori by Gram staining and positive oxidase, catalase, and urease tests. Various motif types of cagA and the prevalence of dupA were determined by PCR method.

Results: Out of 280 specimens, 128 (54.7%) isolated organisms were identified as H. pylori. Of 120 H. pylori isolates, 35.9% were dupA positive and 56.26% were cagA positive, while cagA with ABC and ABCC motifs were 55.5% and 44.5%, respectively. Fifty six percent of the isolates with the ABCC motif have had dupA genes. We also found a significant association between strains with genotypes of dupA-ABC and duodenal ulcer disease (p = 0.007).

Conclusion: The results of this study showed that the prevalence of cagA-positive H. pylori in Shiraz was as high as in western countries and higher numbers of EPIYA-C segments were seen in gastric cancer patients. We may also use dupA as a prognostic and pathogenic marker for duodenal ulcer disease and cagA with the segment C for gastric cancer and gastric ulcer disease in this region.

No MeSH data available.


Related in: MedlinePlus