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Sleep deprivation induces changes in immunity in Trichinella spiralis-infected rats.

Ibarra-Coronado EG, Velazquéz-Moctezuma J, Diaz D, Becerril-Villanueva LE, Pavón L, Morales-Montor J - Int. J. Biol. Sci. (2015)

Bottom Line: Our results demonstrate that the sleep deprivation and stress have a differential effect in mesenteric lymph nodes (MLN) and spleen.In the MLN both are also associated to an increase in cytotoxic T cells (CD3+CD8+) and B cells (CD45+).These results support the notion that sleep is a fundamental process for an adequate and strong immune response generated against this parasite.

View Article: PubMed Central - PubMed

Affiliation: 1. Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP 70228, México D.F. 04510, México.

ABSTRACT
Sleep is considered an important predictor of immunity. A lack of sleep may reduce immunity, which increases susceptibility to any type of infection. Moreover, sleep deprivation in humans produces changes in both, the percent of circulating immune cells (T cells and NK cells) and cytokine levels (IL-1, IFNγ, TNΦ-αα, IL-6 and IL-17). The aim of our study was to investigate whether sleep deprivation produces deregulation on immune variables during the immune response generated against the helminth parasite Trichinella spiralis. Because sleep deprivation is stressful per se, we designed another experiments to compared stress alone (consisting in movement restriction and single housing) with sleep deprivation, in both control (uninfected) and experimental (infected) rats. Our results demonstrate that the sleep deprivation and stress have a differential effect in mesenteric lymph nodes (MLN) and spleen. In uninfected rats sleep deprivation alone produces an increase in natural killer cells (NK+) and B cells (CD45+), accompanied by a decrease in cytotoxic T cells (CD3+CD8+) in spleen; while, in MLN, produces only an increase in natural killer cells (NK+). Both, SD and stress, produce an increased percentage of total T cells (CD3+) in spleen. In the MLN both are also associated to an increase in cytotoxic T cells (CD3+CD8+) and B cells (CD45+). In the spleens of parasitized rats, cell populations did not change. In spleens of both, sleep-deprived and stressed infected rats, we observed an increase in B cells (CD45+). In infected rats, sleep deprivation alone produced an increase in NK cells (NK+). In mesenteric node cell populations of parasitized rats, we observed a decrease in NK cells and an increase in T helper (CD4+) cells in both SD and stressed rats. Rats that were only subjected to stress showed a decrease in B cells (CD45+). These findings suggest that the immune response generated against infection caused by T. spiralis is affected when the sleep pattern is disrupted. These results support the notion that sleep is a fundamental process for an adequate and strong immune response generated against this parasite.

No MeSH data available.


Related in: MedlinePlus

Helper T cells (CD3+CD4+) in the mesenteric lymph nodes (MLN) and spleen. Sleep-sufficient (open bars), stress (shaded bars) and sleep deprivation (SD) (solid bars) conditions in the MLN (A) and spleen (C) were measured in infected and uninfected groups. Panels B and D show the differential effect of infection in the three study conditions: sleep sufficiency, stress and SD in the MLN (B) and spleen (D). The dashed red line represents the percentage of T cells (CD3+) in the uninfected groups; the solid blue line represents the percentage of T cells in the infected groups. *p<0.05%; the percentage of change with respect to the uninfected group is shown. In infected groups, the SD and stress conditions increased the percentage of helper T cells compared to the condition of sleep sufficiency in the MLN, whereas in the spleen, only stress induced an increase compared to the sleep-sufficient condition. The infection induced a significant decrease in the sleep-sufficient condition and an increase in the SD condition in the MLN, whereas in the spleen, a significant increase was only observed in the stress condition. Experimental group were shown as an average ± standard error and analysed by means of a two way-ANOVA and Bonferroni as a post hoc test. Differences were considered significant when p< 0.05.
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Figure 3: Helper T cells (CD3+CD4+) in the mesenteric lymph nodes (MLN) and spleen. Sleep-sufficient (open bars), stress (shaded bars) and sleep deprivation (SD) (solid bars) conditions in the MLN (A) and spleen (C) were measured in infected and uninfected groups. Panels B and D show the differential effect of infection in the three study conditions: sleep sufficiency, stress and SD in the MLN (B) and spleen (D). The dashed red line represents the percentage of T cells (CD3+) in the uninfected groups; the solid blue line represents the percentage of T cells in the infected groups. *p<0.05%; the percentage of change with respect to the uninfected group is shown. In infected groups, the SD and stress conditions increased the percentage of helper T cells compared to the condition of sleep sufficiency in the MLN, whereas in the spleen, only stress induced an increase compared to the sleep-sufficient condition. The infection induced a significant decrease in the sleep-sufficient condition and an increase in the SD condition in the MLN, whereas in the spleen, a significant increase was only observed in the stress condition. Experimental group were shown as an average ± standard error and analysed by means of a two way-ANOVA and Bonferroni as a post hoc test. Differences were considered significant when p< 0.05.

Mentions: The proportions of the T cell subpopulations were analysed from the total population of T cells (CD3+). The proportions of T helper cells (CD3+, CD4+) and cytotoxic T cells (CD3+, CD8+) are shown in panels (Fig 3 and 4). For the T helper cell population (CD4+), in infected rats we observed in the MLN an increase of 23 and 31% (p <0.05) for the SD and stress conditions, respectively (Fig. 3A and 3B). In spleens of infected animals, stress condition caused also an increase of 19% in CD4+ lymphocytes (p <0.05).


Sleep deprivation induces changes in immunity in Trichinella spiralis-infected rats.

Ibarra-Coronado EG, Velazquéz-Moctezuma J, Diaz D, Becerril-Villanueva LE, Pavón L, Morales-Montor J - Int. J. Biol. Sci. (2015)

Helper T cells (CD3+CD4+) in the mesenteric lymph nodes (MLN) and spleen. Sleep-sufficient (open bars), stress (shaded bars) and sleep deprivation (SD) (solid bars) conditions in the MLN (A) and spleen (C) were measured in infected and uninfected groups. Panels B and D show the differential effect of infection in the three study conditions: sleep sufficiency, stress and SD in the MLN (B) and spleen (D). The dashed red line represents the percentage of T cells (CD3+) in the uninfected groups; the solid blue line represents the percentage of T cells in the infected groups. *p<0.05%; the percentage of change with respect to the uninfected group is shown. In infected groups, the SD and stress conditions increased the percentage of helper T cells compared to the condition of sleep sufficiency in the MLN, whereas in the spleen, only stress induced an increase compared to the sleep-sufficient condition. The infection induced a significant decrease in the sleep-sufficient condition and an increase in the SD condition in the MLN, whereas in the spleen, a significant increase was only observed in the stress condition. Experimental group were shown as an average ± standard error and analysed by means of a two way-ANOVA and Bonferroni as a post hoc test. Differences were considered significant when p< 0.05.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4495408&req=5

Figure 3: Helper T cells (CD3+CD4+) in the mesenteric lymph nodes (MLN) and spleen. Sleep-sufficient (open bars), stress (shaded bars) and sleep deprivation (SD) (solid bars) conditions in the MLN (A) and spleen (C) were measured in infected and uninfected groups. Panels B and D show the differential effect of infection in the three study conditions: sleep sufficiency, stress and SD in the MLN (B) and spleen (D). The dashed red line represents the percentage of T cells (CD3+) in the uninfected groups; the solid blue line represents the percentage of T cells in the infected groups. *p<0.05%; the percentage of change with respect to the uninfected group is shown. In infected groups, the SD and stress conditions increased the percentage of helper T cells compared to the condition of sleep sufficiency in the MLN, whereas in the spleen, only stress induced an increase compared to the sleep-sufficient condition. The infection induced a significant decrease in the sleep-sufficient condition and an increase in the SD condition in the MLN, whereas in the spleen, a significant increase was only observed in the stress condition. Experimental group were shown as an average ± standard error and analysed by means of a two way-ANOVA and Bonferroni as a post hoc test. Differences were considered significant when p< 0.05.
Mentions: The proportions of the T cell subpopulations were analysed from the total population of T cells (CD3+). The proportions of T helper cells (CD3+, CD4+) and cytotoxic T cells (CD3+, CD8+) are shown in panels (Fig 3 and 4). For the T helper cell population (CD4+), in infected rats we observed in the MLN an increase of 23 and 31% (p <0.05) for the SD and stress conditions, respectively (Fig. 3A and 3B). In spleens of infected animals, stress condition caused also an increase of 19% in CD4+ lymphocytes (p <0.05).

Bottom Line: Our results demonstrate that the sleep deprivation and stress have a differential effect in mesenteric lymph nodes (MLN) and spleen.In the MLN both are also associated to an increase in cytotoxic T cells (CD3+CD8+) and B cells (CD45+).These results support the notion that sleep is a fundamental process for an adequate and strong immune response generated against this parasite.

View Article: PubMed Central - PubMed

Affiliation: 1. Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP 70228, México D.F. 04510, México.

ABSTRACT
Sleep is considered an important predictor of immunity. A lack of sleep may reduce immunity, which increases susceptibility to any type of infection. Moreover, sleep deprivation in humans produces changes in both, the percent of circulating immune cells (T cells and NK cells) and cytokine levels (IL-1, IFNγ, TNΦ-αα, IL-6 and IL-17). The aim of our study was to investigate whether sleep deprivation produces deregulation on immune variables during the immune response generated against the helminth parasite Trichinella spiralis. Because sleep deprivation is stressful per se, we designed another experiments to compared stress alone (consisting in movement restriction and single housing) with sleep deprivation, in both control (uninfected) and experimental (infected) rats. Our results demonstrate that the sleep deprivation and stress have a differential effect in mesenteric lymph nodes (MLN) and spleen. In uninfected rats sleep deprivation alone produces an increase in natural killer cells (NK+) and B cells (CD45+), accompanied by a decrease in cytotoxic T cells (CD3+CD8+) in spleen; while, in MLN, produces only an increase in natural killer cells (NK+). Both, SD and stress, produce an increased percentage of total T cells (CD3+) in spleen. In the MLN both are also associated to an increase in cytotoxic T cells (CD3+CD8+) and B cells (CD45+). In the spleens of parasitized rats, cell populations did not change. In spleens of both, sleep-deprived and stressed infected rats, we observed an increase in B cells (CD45+). In infected rats, sleep deprivation alone produced an increase in NK cells (NK+). In mesenteric node cell populations of parasitized rats, we observed a decrease in NK cells and an increase in T helper (CD4+) cells in both SD and stressed rats. Rats that were only subjected to stress showed a decrease in B cells (CD45+). These findings suggest that the immune response generated against infection caused by T. spiralis is affected when the sleep pattern is disrupted. These results support the notion that sleep is a fundamental process for an adequate and strong immune response generated against this parasite.

No MeSH data available.


Related in: MedlinePlus