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Silver nanoparticle exposure induced mitochondrial stress, caspase-3 activation and cell death: amelioration by sodium selenite.

Ma W, Jing L, Valladares A, Mehta SL, Wang Z, Li PA, Bang JJ - Int. J. Biol. Sci. (2015)

Bottom Line: Selenium, an essential trace element that has been known to carry protecting property from heavy metals, was tested for its ameliorating potential in the cells exposed to AgNP.Results showed that AgNP reduced cell viability.It is concluded that AgNP induces mitochondrial stress and treatment with selenite is capable of preventing the adverse effects of AgNP on the mitochondria.

View Article: PubMed Central - PubMed

Affiliation: 1. Department of Comprehensive Medicine, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, P.R. China ; 4. Department of Pharmaceutical Sciences, Biomanufacturing Research Institute and Technological Enterprise (BRITE), North Carolina Central University, Durham, North Carolina, USA.

ABSTRACT
Silver nanoparticles (AgNP), one of the most commonly used engineered nanomaterial for biomedical and industrial applications, has shown a toxic potential to our ecosystems and humans. In this study, murine hippocampal neuronal HT22 cells were used to delineate subcellular responses and mechanisms to AgNP by assessing the response levels of caspase-3, mitochondrial oxygen consumption, reactive oxygen species (ROS), and mitochondrial membrane potential in addition to cell viability testing. Selenium, an essential trace element that has been known to carry protecting property from heavy metals, was tested for its ameliorating potential in the cells exposed to AgNP. Results showed that AgNP reduced cell viability. The toxicity was associated with mitochondrial membrane depolarization, increased accumulation of ROS, elevated mitochondrial oxygen consumption, and caspase-3 activation. Treatment with sodium selenite reduced cell death, stabilized mitochondrial membrane potential and oxygen consumption rate, and prevented accumulation of ROS and activation of caspase-3. It is concluded that AgNP induces mitochondrial stress and treatment with selenite is capable of preventing the adverse effects of AgNP on the mitochondria.

No MeSH data available.


Related in: MedlinePlus

AgNP cell toxicity detected using alamar blue assay. The viability decreased with increased concentrations of AgNP. Data were collected from 3 or more independent experiments and presented as means ± s.d. One-way ANOVA followed by post-hoc Scheffe's test. *p<0.001 vs. control.
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Figure 2: AgNP cell toxicity detected using alamar blue assay. The viability decreased with increased concentrations of AgNP. Data were collected from 3 or more independent experiments and presented as means ± s.d. One-way ANOVA followed by post-hoc Scheffe's test. *p<0.001 vs. control.

Mentions: The cell toxicity of AgNP was determined after 24 h following various amounts of nano-siver particle incubation. The results showed that there was an inversed linear relationship between the concentration of AgNP and cell viability. As shown in Figure 2, at a low concentration of 0.1 ppm, AgNP did not induce cell death. When its concentration increased to 1.0 ppm, the viability decreased to 60%, and further declined to 35% and 10% when the AgNP increased to 2.0 ppm and 5.0 ppm, respectively.


Silver nanoparticle exposure induced mitochondrial stress, caspase-3 activation and cell death: amelioration by sodium selenite.

Ma W, Jing L, Valladares A, Mehta SL, Wang Z, Li PA, Bang JJ - Int. J. Biol. Sci. (2015)

AgNP cell toxicity detected using alamar blue assay. The viability decreased with increased concentrations of AgNP. Data were collected from 3 or more independent experiments and presented as means ± s.d. One-way ANOVA followed by post-hoc Scheffe's test. *p<0.001 vs. control.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4495404&req=5

Figure 2: AgNP cell toxicity detected using alamar blue assay. The viability decreased with increased concentrations of AgNP. Data were collected from 3 or more independent experiments and presented as means ± s.d. One-way ANOVA followed by post-hoc Scheffe's test. *p<0.001 vs. control.
Mentions: The cell toxicity of AgNP was determined after 24 h following various amounts of nano-siver particle incubation. The results showed that there was an inversed linear relationship between the concentration of AgNP and cell viability. As shown in Figure 2, at a low concentration of 0.1 ppm, AgNP did not induce cell death. When its concentration increased to 1.0 ppm, the viability decreased to 60%, and further declined to 35% and 10% when the AgNP increased to 2.0 ppm and 5.0 ppm, respectively.

Bottom Line: Selenium, an essential trace element that has been known to carry protecting property from heavy metals, was tested for its ameliorating potential in the cells exposed to AgNP.Results showed that AgNP reduced cell viability.It is concluded that AgNP induces mitochondrial stress and treatment with selenite is capable of preventing the adverse effects of AgNP on the mitochondria.

View Article: PubMed Central - PubMed

Affiliation: 1. Department of Comprehensive Medicine, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, P.R. China ; 4. Department of Pharmaceutical Sciences, Biomanufacturing Research Institute and Technological Enterprise (BRITE), North Carolina Central University, Durham, North Carolina, USA.

ABSTRACT
Silver nanoparticles (AgNP), one of the most commonly used engineered nanomaterial for biomedical and industrial applications, has shown a toxic potential to our ecosystems and humans. In this study, murine hippocampal neuronal HT22 cells were used to delineate subcellular responses and mechanisms to AgNP by assessing the response levels of caspase-3, mitochondrial oxygen consumption, reactive oxygen species (ROS), and mitochondrial membrane potential in addition to cell viability testing. Selenium, an essential trace element that has been known to carry protecting property from heavy metals, was tested for its ameliorating potential in the cells exposed to AgNP. Results showed that AgNP reduced cell viability. The toxicity was associated with mitochondrial membrane depolarization, increased accumulation of ROS, elevated mitochondrial oxygen consumption, and caspase-3 activation. Treatment with sodium selenite reduced cell death, stabilized mitochondrial membrane potential and oxygen consumption rate, and prevented accumulation of ROS and activation of caspase-3. It is concluded that AgNP induces mitochondrial stress and treatment with selenite is capable of preventing the adverse effects of AgNP on the mitochondria.

No MeSH data available.


Related in: MedlinePlus