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The effects of age and ganglioside composition on the rate of motor nerve terminal regeneration following antibody-mediated injury in mice.

Rupp A, Cunningham ME, Yao D, Furukawa K, Willison HJ - Synapse (2013)

Bottom Line: Gangliosides are glycosphingolipids highly enriched in neural plasma membranes, where they mediate a diverse range of functions and can act as targets for auto-antibodies present in human immune-mediated neuropathy sera.Both aging and ganglioside-deficiency have been linked to impaired axonal regeneration.Five days later, most NMJs, regardless of age and strain, had recovered their mNTs.

View Article: PubMed Central - PubMed

Affiliation: Neuroimmunology Group, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow, G12 8TA, United Kingdom.

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Extent of mNT injury and regeneration (assessed by CFP overlying the NMJ) following application of anti-ganglioside Abs and complement in young adult WT, GD3sKO, and GM2sKO mice. Due to ganglioside expression and specificity of the anti-ganglioside Abs applied, WT-1 and GD3sKO mice experienced a selective mNT injury, whereas WT-2 and GM2sKO mice experienced a combined mNT and pSC injury. Following 5 days of regeneration, most NMJs under examination exhibited CFP and the values obtained in the experimental groups tallied very closely with their control counterparts. In the two KO strains, no statistically significant differences were observed between experimental and control tissue (GD3sKO: P = 0.21, Fisher's exact test, n = 3; GM2sKO: P = 0.19, Fisher's exact test, n = 3), whereas, in the two WT groups, statistically significant differences were observed between experimental and control tissue (P < 0.0001, Chi-square test, n = 3). Data for each group is presented as mean and standard error of the mean.
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fig03: Extent of mNT injury and regeneration (assessed by CFP overlying the NMJ) following application of anti-ganglioside Abs and complement in young adult WT, GD3sKO, and GM2sKO mice. Due to ganglioside expression and specificity of the anti-ganglioside Abs applied, WT-1 and GD3sKO mice experienced a selective mNT injury, whereas WT-2 and GM2sKO mice experienced a combined mNT and pSC injury. Following 5 days of regeneration, most NMJs under examination exhibited CFP and the values obtained in the experimental groups tallied very closely with their control counterparts. In the two KO strains, no statistically significant differences were observed between experimental and control tissue (GD3sKO: P = 0.21, Fisher's exact test, n = 3; GM2sKO: P = 0.19, Fisher's exact test, n = 3), whereas, in the two WT groups, statistically significant differences were observed between experimental and control tissue (P < 0.0001, Chi-square test, n = 3). Data for each group is presented as mean and standard error of the mean.

Mentions: The extent of mNT injury following an anti-ganglioside Ab and complement-mediated injury differed between the various mouse strains (Fig. 3) and statistically significant differences were observed when comparing strains which had been subjected to the same type of injury (WT-1 vs. GD3sKO: P = 0.0001; WT-2 vs. GM2sKO: P < 0.0001). The average percentage of NMJs still exhibiting cytosolic CFP (i.e., intact mNT) following induction of the injury ranged from 1.5% (GD3sKO) to 11.8% (WT-2).


The effects of age and ganglioside composition on the rate of motor nerve terminal regeneration following antibody-mediated injury in mice.

Rupp A, Cunningham ME, Yao D, Furukawa K, Willison HJ - Synapse (2013)

Extent of mNT injury and regeneration (assessed by CFP overlying the NMJ) following application of anti-ganglioside Abs and complement in young adult WT, GD3sKO, and GM2sKO mice. Due to ganglioside expression and specificity of the anti-ganglioside Abs applied, WT-1 and GD3sKO mice experienced a selective mNT injury, whereas WT-2 and GM2sKO mice experienced a combined mNT and pSC injury. Following 5 days of regeneration, most NMJs under examination exhibited CFP and the values obtained in the experimental groups tallied very closely with their control counterparts. In the two KO strains, no statistically significant differences were observed between experimental and control tissue (GD3sKO: P = 0.21, Fisher's exact test, n = 3; GM2sKO: P = 0.19, Fisher's exact test, n = 3), whereas, in the two WT groups, statistically significant differences were observed between experimental and control tissue (P < 0.0001, Chi-square test, n = 3). Data for each group is presented as mean and standard error of the mean.
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Related In: Results  -  Collection

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fig03: Extent of mNT injury and regeneration (assessed by CFP overlying the NMJ) following application of anti-ganglioside Abs and complement in young adult WT, GD3sKO, and GM2sKO mice. Due to ganglioside expression and specificity of the anti-ganglioside Abs applied, WT-1 and GD3sKO mice experienced a selective mNT injury, whereas WT-2 and GM2sKO mice experienced a combined mNT and pSC injury. Following 5 days of regeneration, most NMJs under examination exhibited CFP and the values obtained in the experimental groups tallied very closely with their control counterparts. In the two KO strains, no statistically significant differences were observed between experimental and control tissue (GD3sKO: P = 0.21, Fisher's exact test, n = 3; GM2sKO: P = 0.19, Fisher's exact test, n = 3), whereas, in the two WT groups, statistically significant differences were observed between experimental and control tissue (P < 0.0001, Chi-square test, n = 3). Data for each group is presented as mean and standard error of the mean.
Mentions: The extent of mNT injury following an anti-ganglioside Ab and complement-mediated injury differed between the various mouse strains (Fig. 3) and statistically significant differences were observed when comparing strains which had been subjected to the same type of injury (WT-1 vs. GD3sKO: P = 0.0001; WT-2 vs. GM2sKO: P < 0.0001). The average percentage of NMJs still exhibiting cytosolic CFP (i.e., intact mNT) following induction of the injury ranged from 1.5% (GD3sKO) to 11.8% (WT-2).

Bottom Line: Gangliosides are glycosphingolipids highly enriched in neural plasma membranes, where they mediate a diverse range of functions and can act as targets for auto-antibodies present in human immune-mediated neuropathy sera.Both aging and ganglioside-deficiency have been linked to impaired axonal regeneration.Five days later, most NMJs, regardless of age and strain, had recovered their mNTs.

View Article: PubMed Central - PubMed

Affiliation: Neuroimmunology Group, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow, G12 8TA, United Kingdom.

Show MeSH
Related in: MedlinePlus