The effects of age and ganglioside composition on the rate of motor nerve terminal regeneration following antibody-mediated injury in mice.
Bottom Line: Gangliosides are glycosphingolipids highly enriched in neural plasma membranes, where they mediate a diverse range of functions and can act as targets for auto-antibodies present in human immune-mediated neuropathy sera.Both aging and ganglioside-deficiency have been linked to impaired axonal regeneration.Five days later, most NMJs, regardless of age and strain, had recovered their mNTs.
Affiliation: Neuroimmunology Group, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow, G12 8TA, United Kingdom.Show MeSH
Related in: MedlinePlus
Mentions: Depending on the binding specificity of the anti-ganglioside Ab applied, a selective injury to the mNTs (loss of CFP overlying the NMJ) or a combined injury to the mNTs and pSCs (loss of both CFP and GFP overlying the NMJ) was observed (Fig. 2). In WT mice, the application of TBG3 and complement was associated with a selective mNT injury (WT-1), whereas the combined application of TBG3 and EG1 lead to a combined mNT and pSC injury (WT-2). In GM2sKO mice, the application of EG1 and complement elicited a loss of both CFP and GFP, thus inducing a combined mNT and pSC injury, and in GD3sKO mice, the application of TBG3 and complement lead to a selective loss of CFP, thus inducing a selective mNT injury. The application of human serum as a source of complement (in the absence of anti-ganglioside Ab) and exposure of muscles to in vivo imaging procedures did not induce any changes to the fluorescent proteins of the NMJ (Table1; Fig. 2).
Affiliation: Neuroimmunology Group, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow, G12 8TA, United Kingdom.