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A Duo of Potassium-Responsive Histidine Kinases Govern the Multicellular Destiny of Bacillus subtilis.

Grau RR, de Oña P, Kunert M, Leñini C, Gallegos-Monterrosa R, Mhatre E, Vileta D, Donato V, Hölscher T, Boland W, Kuipers OP, Kovács ÁT - MBio (2015)

Bottom Line: Overall, these results provide insights into how multicellular behaviors formerly believed to be antagonistic are coordinately activated in benefit of the bacterium and its interaction with the host.Here, we use the model plant-beneficial bacterium Bacillus subtilis to answer this question.The spatiotemporal response of these kinases to variable potassium levels and the gradual increase in Spo0A~Pi levels that orchestrates the activation of sliding before biofilm formation shed light on how multicellular behaviors formerly believed to be antagonistic work together to benefit the population fitness.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas (FCByF), Universidad Nacional de Rosario (UNR)-CONICET, Argentina robertograu@fulbrightmail.org akos-tibor.kovacs@uni-jena.de.

No MeSH data available.


Related in: MedlinePlus

De novo branched fatty acid synthesis is required for swarming and sliding proficiencies in B. subtilis. (A) Dose-dependent inhibitory effect of sub-MICs of cerulenin on swarming and sliding proficiencies of NCIB3610-related wild-type and bslA and hag mutant strains, respectively. Sliding and swarming experiments were performed as indicated in the legend to Fig. 1 but with the inclusion of the indicated cerulenin concentration in the soft agar plates. (B) Exogenous branched FAs but not linear FAs (palmitic [nC16:0] and oleic [nC18:1] acids) restore the sliding proficiency of the B. subtilis natto strain in the absence of de novo FA synthesis.
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fig4: De novo branched fatty acid synthesis is required for swarming and sliding proficiencies in B. subtilis. (A) Dose-dependent inhibitory effect of sub-MICs of cerulenin on swarming and sliding proficiencies of NCIB3610-related wild-type and bslA and hag mutant strains, respectively. Sliding and swarming experiments were performed as indicated in the legend to Fig. 1 but with the inclusion of the indicated cerulenin concentration in the soft agar plates. (B) Exogenous branched FAs but not linear FAs (palmitic [nC16:0] and oleic [nC18:1] acids) restore the sliding proficiency of the B. subtilis natto strain in the absence of de novo FA synthesis.

Mentions: While the genes involved in fatty acid (FA) synthesis (i.e., fabF, fabHBA, fabG, etc.) were overexpressed in wild-type cells under sliding-permissive conditions, those genes involved in FA degradation (i.e., fadR, fadA, fadE, etc.) were downregulated at the same time (see Table S2 in the supplemental material). Is an active lipid synthesis, and therefore active membrane formation and remodeling, necessary to slide? In order to confirm the in silico results and test the formulated hypothesis, we proceeded to specifically block de novo FA synthesis in B. subtilis cells grown under swarming- and sliding-supportive conditions. To this end, we treated B. subtilis cells with the antibiotic cerulenin, which is a specific inhibitor of the FabF condensing enzyme (14), at sub-MICs (below 2 µg ⋅ ml−1), which do not affect the vegetative growth of the NCIB3610 and RG4365 strains (50) (see Fig. S3A and B). Our results show that sub-MICs of cerulenin produce a dose-dependent impediment of sliding motility as well as swarming in B. subtilis (Fig. 4A; also see Fig. S3C and D). These results confirm the microarray data and suggest that an active de novo FA synthesis constitutes an overlooked requirement for surface (sliding and swarming) motility.


A Duo of Potassium-Responsive Histidine Kinases Govern the Multicellular Destiny of Bacillus subtilis.

Grau RR, de Oña P, Kunert M, Leñini C, Gallegos-Monterrosa R, Mhatre E, Vileta D, Donato V, Hölscher T, Boland W, Kuipers OP, Kovács ÁT - MBio (2015)

De novo branched fatty acid synthesis is required for swarming and sliding proficiencies in B. subtilis. (A) Dose-dependent inhibitory effect of sub-MICs of cerulenin on swarming and sliding proficiencies of NCIB3610-related wild-type and bslA and hag mutant strains, respectively. Sliding and swarming experiments were performed as indicated in the legend to Fig. 1 but with the inclusion of the indicated cerulenin concentration in the soft agar plates. (B) Exogenous branched FAs but not linear FAs (palmitic [nC16:0] and oleic [nC18:1] acids) restore the sliding proficiency of the B. subtilis natto strain in the absence of de novo FA synthesis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4495169&req=5

fig4: De novo branched fatty acid synthesis is required for swarming and sliding proficiencies in B. subtilis. (A) Dose-dependent inhibitory effect of sub-MICs of cerulenin on swarming and sliding proficiencies of NCIB3610-related wild-type and bslA and hag mutant strains, respectively. Sliding and swarming experiments were performed as indicated in the legend to Fig. 1 but with the inclusion of the indicated cerulenin concentration in the soft agar plates. (B) Exogenous branched FAs but not linear FAs (palmitic [nC16:0] and oleic [nC18:1] acids) restore the sliding proficiency of the B. subtilis natto strain in the absence of de novo FA synthesis.
Mentions: While the genes involved in fatty acid (FA) synthesis (i.e., fabF, fabHBA, fabG, etc.) were overexpressed in wild-type cells under sliding-permissive conditions, those genes involved in FA degradation (i.e., fadR, fadA, fadE, etc.) were downregulated at the same time (see Table S2 in the supplemental material). Is an active lipid synthesis, and therefore active membrane formation and remodeling, necessary to slide? In order to confirm the in silico results and test the formulated hypothesis, we proceeded to specifically block de novo FA synthesis in B. subtilis cells grown under swarming- and sliding-supportive conditions. To this end, we treated B. subtilis cells with the antibiotic cerulenin, which is a specific inhibitor of the FabF condensing enzyme (14), at sub-MICs (below 2 µg ⋅ ml−1), which do not affect the vegetative growth of the NCIB3610 and RG4365 strains (50) (see Fig. S3A and B). Our results show that sub-MICs of cerulenin produce a dose-dependent impediment of sliding motility as well as swarming in B. subtilis (Fig. 4A; also see Fig. S3C and D). These results confirm the microarray data and suggest that an active de novo FA synthesis constitutes an overlooked requirement for surface (sliding and swarming) motility.

Bottom Line: Overall, these results provide insights into how multicellular behaviors formerly believed to be antagonistic are coordinately activated in benefit of the bacterium and its interaction with the host.Here, we use the model plant-beneficial bacterium Bacillus subtilis to answer this question.The spatiotemporal response of these kinases to variable potassium levels and the gradual increase in Spo0A~Pi levels that orchestrates the activation of sliding before biofilm formation shed light on how multicellular behaviors formerly believed to be antagonistic work together to benefit the population fitness.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas (FCByF), Universidad Nacional de Rosario (UNR)-CONICET, Argentina robertograu@fulbrightmail.org akos-tibor.kovacs@uni-jena.de.

No MeSH data available.


Related in: MedlinePlus