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Influence of different intensities of vibration on proliferation and differentiation of human periodontal ligament stem cells.

Zhang C, Lu Y, Zhang L, Liu Y, Zhou Y, Chen Y, Yu H - Arch Med Sci (2015)

Bottom Line: The expression of alkaline phosphatase (ALP) and osteocalcin (OCN) mRNA was up-regulated at 0.1 g, 0.3 g, 0.6 g and 0.9 g magnitude, with the peak at 0.3 g.The expression levels of both mRNA and protein of Runx2 and osterix (OSX) significantly increased at a magnitude of 0.1 g to 0.9 g, reached a peak at 0.3 g and then decreased slowly.The scleraxis, tenogenic markers, and mRNA expression decreased at 0.05 g, 0.1 g, and 0.3 g, and significantly increased at 0.6 g and 0.9 g.

View Article: PubMed Central - PubMed

Affiliation: Tianjin Stomatological Hospital of Nankai University, Tianjin, China.

ABSTRACT

Introduction: To understand the effects of low-magnitude, high-frequency (LMHF) mechanical vibration at different intensities on human periodontal ligament stem cell (hPDLSC) proliferation and osteogenic differentiation.

Material and methods: The effect of vibration on hPDLSC proliferation, osteogenic differentiation, tenogenic differentiation and cytoskeleton was assessed at the cellular, genetic and protein level.

Results: The PDLSC proliferation was decreased after different magnitudes of mechanical vibration; however, there were no obvious senescent cells in the experimental and the static control group. Expression of osteogenesis markers was increased. The expression of alkaline phosphatase (ALP) and osteocalcin (OCN) mRNA was up-regulated at 0.1 g, 0.3 g, 0.6 g and 0.9 g magnitude, with the peak at 0.3 g. The type I collagen (Col-I) level was increased after vibration exposure at 0.1 g, 0.3 g, and 0.6 g, peaking at 0.3 g. The expression levels of both mRNA and protein of Runx2 and osterix (OSX) significantly increased at a magnitude of 0.1 g to 0.9 g, reached a peak at 0.3 g and then decreased slowly. The scleraxis, tenogenic markers, and mRNA expression decreased at 0.05 g, 0.1 g, and 0.3 g, and significantly increased at 0.6 g and 0.9 g. Compared with the static group, the F-actin stress fibers of hPDLSCs became thicker and clearer following vibration.

Conclusions: The LMHF mechanical vibration promotes PDLSC osteogenic differentiation and implies the existence of a magnitude-dependent effect of vibration on determining PDLSC commitment to the osteoblast lineage. Changes in the cytoskeleton of hPDLSCs after vibration may be one of the mechanisms of the biological effects.

No MeSH data available.


Related in: MedlinePlus

The effect of mechanical vibration at different magnitudes on tenogenic gene expression in hPDLSCs. Quantitative PCR results indicate that the mRNA expression level of Scleraxis, a tendon specific transcription factor, decreased at 0.05 g, 0.1 g, and 0.3 g, and significantly increased at 0.6 g and 0.9 g, with a peak increase at 0.9 gEach bar represents the mean ± standard deviation (n = 3); *p < 0.05.
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Figure 0003: The effect of mechanical vibration at different magnitudes on tenogenic gene expression in hPDLSCs. Quantitative PCR results indicate that the mRNA expression level of Scleraxis, a tendon specific transcription factor, decreased at 0.05 g, 0.1 g, and 0.3 g, and significantly increased at 0.6 g and 0.9 g, with a peak increase at 0.9 gEach bar represents the mean ± standard deviation (n = 3); *p < 0.05.

Mentions: The PDLSCs express both osteogenic and tenogenic phenotypes. The mRNA expression level of scleraxis, a tendon-specific transcription factor, decreased at 0.05 g, 0.1 g, and 0.3 g, and significantly increased at 0.6 g and 0.9 g, with a peak increase at 0.9 g (1.97-fold increase versus control; p < 0.05) (Figure 3).


Influence of different intensities of vibration on proliferation and differentiation of human periodontal ligament stem cells.

Zhang C, Lu Y, Zhang L, Liu Y, Zhou Y, Chen Y, Yu H - Arch Med Sci (2015)

The effect of mechanical vibration at different magnitudes on tenogenic gene expression in hPDLSCs. Quantitative PCR results indicate that the mRNA expression level of Scleraxis, a tendon specific transcription factor, decreased at 0.05 g, 0.1 g, and 0.3 g, and significantly increased at 0.6 g and 0.9 g, with a peak increase at 0.9 gEach bar represents the mean ± standard deviation (n = 3); *p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4495159&req=5

Figure 0003: The effect of mechanical vibration at different magnitudes on tenogenic gene expression in hPDLSCs. Quantitative PCR results indicate that the mRNA expression level of Scleraxis, a tendon specific transcription factor, decreased at 0.05 g, 0.1 g, and 0.3 g, and significantly increased at 0.6 g and 0.9 g, with a peak increase at 0.9 gEach bar represents the mean ± standard deviation (n = 3); *p < 0.05.
Mentions: The PDLSCs express both osteogenic and tenogenic phenotypes. The mRNA expression level of scleraxis, a tendon-specific transcription factor, decreased at 0.05 g, 0.1 g, and 0.3 g, and significantly increased at 0.6 g and 0.9 g, with a peak increase at 0.9 g (1.97-fold increase versus control; p < 0.05) (Figure 3).

Bottom Line: The expression of alkaline phosphatase (ALP) and osteocalcin (OCN) mRNA was up-regulated at 0.1 g, 0.3 g, 0.6 g and 0.9 g magnitude, with the peak at 0.3 g.The expression levels of both mRNA and protein of Runx2 and osterix (OSX) significantly increased at a magnitude of 0.1 g to 0.9 g, reached a peak at 0.3 g and then decreased slowly.The scleraxis, tenogenic markers, and mRNA expression decreased at 0.05 g, 0.1 g, and 0.3 g, and significantly increased at 0.6 g and 0.9 g.

View Article: PubMed Central - PubMed

Affiliation: Tianjin Stomatological Hospital of Nankai University, Tianjin, China.

ABSTRACT

Introduction: To understand the effects of low-magnitude, high-frequency (LMHF) mechanical vibration at different intensities on human periodontal ligament stem cell (hPDLSC) proliferation and osteogenic differentiation.

Material and methods: The effect of vibration on hPDLSC proliferation, osteogenic differentiation, tenogenic differentiation and cytoskeleton was assessed at the cellular, genetic and protein level.

Results: The PDLSC proliferation was decreased after different magnitudes of mechanical vibration; however, there were no obvious senescent cells in the experimental and the static control group. Expression of osteogenesis markers was increased. The expression of alkaline phosphatase (ALP) and osteocalcin (OCN) mRNA was up-regulated at 0.1 g, 0.3 g, 0.6 g and 0.9 g magnitude, with the peak at 0.3 g. The type I collagen (Col-I) level was increased after vibration exposure at 0.1 g, 0.3 g, and 0.6 g, peaking at 0.3 g. The expression levels of both mRNA and protein of Runx2 and osterix (OSX) significantly increased at a magnitude of 0.1 g to 0.9 g, reached a peak at 0.3 g and then decreased slowly. The scleraxis, tenogenic markers, and mRNA expression decreased at 0.05 g, 0.1 g, and 0.3 g, and significantly increased at 0.6 g and 0.9 g. Compared with the static group, the F-actin stress fibers of hPDLSCs became thicker and clearer following vibration.

Conclusions: The LMHF mechanical vibration promotes PDLSC osteogenic differentiation and implies the existence of a magnitude-dependent effect of vibration on determining PDLSC commitment to the osteoblast lineage. Changes in the cytoskeleton of hPDLSCs after vibration may be one of the mechanisms of the biological effects.

No MeSH data available.


Related in: MedlinePlus