Limits...
-308 G/A TNF-α gene polymorphism influences the course of basal cell carcinoma in a Polish population.

Sobjanek M, Zabłotna M, Michajłowski I, Nedoszytko B, Lesiak A, Nowicki R - Arch Med Sci (2015)

Bottom Line: There was no statistically significant association between allele, genotype and haplotype frequencies in BCC patients in comparison with controls.The -238/-308 GA haplotype was connected with increased risk of recurrence (OR = 4.36, 95% CI: 1.49-12.7, p = 0.007).We also found significantly higher TNF-α levels among BCC patients in comparison with controls (p = 0.004).

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Venerology and Allergology, Medical University of Gdansk, Gdansk, Poland.

ABSTRACT

Introduction: The etiopathogenesis of basal cell carcinoma (BCC) is multifactorial. The TNF-α gene seems to be an interesting gene candidate for BCC susceptibility because of the proinflammatory and immunosuppressive properties of its product. The aim of the study was to assess the frequency of -308 G/A and -238 G/A gene polymorphisms in the TNF-α gene and serum levels of cytokine in patients with BCC.

Material and methods: The study included 176 (94 women, 82 men) patients with BCC and 261 healthy volunteers. -308 G/A and -238 G/A TNF-α polymorphisms were analyzed using the amplification refractory mutation system-polymerase chain reaction method (ARMS-PCR). Serum concentrations of TNF-α were measured using ELISA.

Results: There was no statistically significant association between allele, genotype and haplotype frequencies in BCC patients in comparison with controls. Occurrence of the -308 TNF-α A allele or GA genotype in the group of patients with BCC increases risk of recurrence of tumor recurrence (OR = 4.8, 95% CI: 1.6-13.9, p = 0.004 and OR = 4.97, 95% CI: 1.7-14.5, p = 0.004). Moreover, -308 TNF-α GG genotype decreased risk of recurrence (OR = 0.2, 95% CI: 0.07-0.6, p = 0.004). The -238/-308 GA haplotype was connected with increased risk of recurrence (OR = 4.36, 95% CI: 1.49-12.7, p = 0.007). We also found significantly higher TNF-α levels among BCC patients in comparison with controls (p = 0.004).

Conclusions: The obtained results did not confirm the role of the -308 G/A and -238 G/A TNF-α gene polymorphisms in BCC development, but the presence of the A allele or GA genotype in -308 G/A TNF-α gene polymorphism may have an impact on the course of the disease.

No MeSH data available.


Related in: MedlinePlus

Allele frequencies of –308 G/A TNF polymorphism in BCC patients with and without tumor recurrence (p = 0.005)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4495156&req=5

Figure 0001: Allele frequencies of –308 G/A TNF polymorphism in BCC patients with and without tumor recurrence (p = 0.005)

Mentions: The genotype distribution of all polymorphisms was in Hardy-Weinberg equilibrium. Table II shows genotypes and alleles frequencies for TNF-α –308 G/A and –238 G/A polymorphisms in patients with BCC and control subjects. There were no statistically significant association between allele, genotype and haplotype frequencies between BCC and control groups. However, AA genotype in –308 G/A TNF-α polymorphisms was statistically more frequent in the group with multiple BCC compared to that with one tumor (3.13 vs. 0%; p = 0.035). In the group of patients with recurrent BCC, GG genotype in –308 G/A TNF-α polymorphism was statistically less common than in the group without recurrence (43.75 vs. 78.81, p = 0.002), whereas GA genotype was more frequent in the recurrent BCC group (56.25 vs. 20.53; p = 0.001). The presence of the A allele or GA genotype for –308 G/A TNF-α polymorphisms significantly increases BCC recurrence risk (OR = 4.8 (95% CI: 1.6–13.9), p = 0.004; OR = 4.97 (95% CI: 1.7–14.5), p = 0.004, respectively). GG genotype in –308 G/A TNF-α polymorphism decreased that risk; OR = 0.2 (95% CI: 0.07–0.6; p = 0.004) (Figures 1, 2).


-308 G/A TNF-α gene polymorphism influences the course of basal cell carcinoma in a Polish population.

Sobjanek M, Zabłotna M, Michajłowski I, Nedoszytko B, Lesiak A, Nowicki R - Arch Med Sci (2015)

Allele frequencies of –308 G/A TNF polymorphism in BCC patients with and without tumor recurrence (p = 0.005)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4495156&req=5

Figure 0001: Allele frequencies of –308 G/A TNF polymorphism in BCC patients with and without tumor recurrence (p = 0.005)
Mentions: The genotype distribution of all polymorphisms was in Hardy-Weinberg equilibrium. Table II shows genotypes and alleles frequencies for TNF-α –308 G/A and –238 G/A polymorphisms in patients with BCC and control subjects. There were no statistically significant association between allele, genotype and haplotype frequencies between BCC and control groups. However, AA genotype in –308 G/A TNF-α polymorphisms was statistically more frequent in the group with multiple BCC compared to that with one tumor (3.13 vs. 0%; p = 0.035). In the group of patients with recurrent BCC, GG genotype in –308 G/A TNF-α polymorphism was statistically less common than in the group without recurrence (43.75 vs. 78.81, p = 0.002), whereas GA genotype was more frequent in the recurrent BCC group (56.25 vs. 20.53; p = 0.001). The presence of the A allele or GA genotype for –308 G/A TNF-α polymorphisms significantly increases BCC recurrence risk (OR = 4.8 (95% CI: 1.6–13.9), p = 0.004; OR = 4.97 (95% CI: 1.7–14.5), p = 0.004, respectively). GG genotype in –308 G/A TNF-α polymorphism decreased that risk; OR = 0.2 (95% CI: 0.07–0.6; p = 0.004) (Figures 1, 2).

Bottom Line: There was no statistically significant association between allele, genotype and haplotype frequencies in BCC patients in comparison with controls.The -238/-308 GA haplotype was connected with increased risk of recurrence (OR = 4.36, 95% CI: 1.49-12.7, p = 0.007).We also found significantly higher TNF-α levels among BCC patients in comparison with controls (p = 0.004).

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology, Venerology and Allergology, Medical University of Gdansk, Gdansk, Poland.

ABSTRACT

Introduction: The etiopathogenesis of basal cell carcinoma (BCC) is multifactorial. The TNF-α gene seems to be an interesting gene candidate for BCC susceptibility because of the proinflammatory and immunosuppressive properties of its product. The aim of the study was to assess the frequency of -308 G/A and -238 G/A gene polymorphisms in the TNF-α gene and serum levels of cytokine in patients with BCC.

Material and methods: The study included 176 (94 women, 82 men) patients with BCC and 261 healthy volunteers. -308 G/A and -238 G/A TNF-α polymorphisms were analyzed using the amplification refractory mutation system-polymerase chain reaction method (ARMS-PCR). Serum concentrations of TNF-α were measured using ELISA.

Results: There was no statistically significant association between allele, genotype and haplotype frequencies in BCC patients in comparison with controls. Occurrence of the -308 TNF-α A allele or GA genotype in the group of patients with BCC increases risk of recurrence of tumor recurrence (OR = 4.8, 95% CI: 1.6-13.9, p = 0.004 and OR = 4.97, 95% CI: 1.7-14.5, p = 0.004). Moreover, -308 TNF-α GG genotype decreased risk of recurrence (OR = 0.2, 95% CI: 0.07-0.6, p = 0.004). The -238/-308 GA haplotype was connected with increased risk of recurrence (OR = 4.36, 95% CI: 1.49-12.7, p = 0.007). We also found significantly higher TNF-α levels among BCC patients in comparison with controls (p = 0.004).

Conclusions: The obtained results did not confirm the role of the -308 G/A and -238 G/A TNF-α gene polymorphisms in BCC development, but the presence of the A allele or GA genotype in -308 G/A TNF-α gene polymorphism may have an impact on the course of the disease.

No MeSH data available.


Related in: MedlinePlus