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MicroRNA-490-5p is a novel tumor suppressor targeting c-FOS in human bladder cancer.

Lan G, Yang L, Xie X, Peng L, Wang Y - Arch Med Sci (2015)

Bottom Line: We found that overexpression of miR-490-5p in T24 cells could inhibit cell proliferation and invasion and induce cell apoptosis.In addition, our bioinformatics prediction and experimental data showed that c-FOS was a potential target of miR-490-5p.The expression level of c-FOS was significantly decreased after miR-490-5p overexpression and significantly increased after miR-490-5p suppression, indicating that c-FOS was a target of miR-490-5p.

View Article: PubMed Central - PubMed

Affiliation: Department of Kidney Transplantation, The Second Xiangya Hospital of Central South University, Changsha, China.

ABSTRACT

Introduction: Recent studies have demonstrated the critical roles of micro-RNAs in tumorigenesis and tumor progression. Here, we describe the regulation and function of miR-490-5p in bladder cancer.

Material and methods: Paired tissue samples were collected from bladder cancer patients (n = 20). Real-time PCR revealed that miR-490-5p expression was significantly down-regulated in human bladder cancer tissues and cells. Also there was an inverse relationship between the expression level of miR-490-5p and the pathological grade of bladder cancer. Western blotting was performed to detect the expression levels of c-FOS and TET1 in 6 matched tumor tissue samples and 4 bladder cell lines. Furthermore, to better understand the underlying mechanisms of miR-490-5p, we conducted gain and loss of function analysis by transfecting bladder cancer T24 cells with chemically synthesized miR-490-5p mimics and inhibitor, respectively.

Results: We found that overexpression of miR-490-5p in T24 cells could inhibit cell proliferation and invasion and induce cell apoptosis. Conversely, suppression of miR-490-5p expression induced cell proliferation and invasion, while it inhibited cell apoptosis. In addition, our bioinformatics prediction and experimental data showed that c-FOS was a potential target of miR-490-5p. The expression level of c-FOS was significantly decreased after miR-490-5p overexpression and significantly increased after miR-490-5p suppression, indicating that c-FOS was a target of miR-490-5p.

Conclusions: These findings suggest that miR-490-5p is a novel tumor suppressor, contributing to the carcinogenesis of bladder cancer by targeting c-FOS.

No MeSH data available.


Related in: MedlinePlus

Effects of miR-490-5p overexpression on c-FOS expression in T24 cells. Quantitative results of western blotting are shown in (A) and representative western blotting results are shown in (B). UVRAG was also blotted and served as an internal control
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Figure 0005: Effects of miR-490-5p overexpression on c-FOS expression in T24 cells. Quantitative results of western blotting are shown in (A) and representative western blotting results are shown in (B). UVRAG was also blotted and served as an internal control

Mentions: We further investigated the effect of miR-490-5p overexpression on the level of c-FOS. Quantitative results of western blotting are shown in Figure 5 A, and representative western blotting results are shown in Figure 5 B. The c-FOS protein level in the miR-490-5p mimics group was significantly lower than that in the other two groups. The effect of miR-490-5p suppression on the level of c-FOS was also observed. The c-FOS protein level in the miR-490-5p inhibitor group was significantly higher than those in the other two groups, as shown in Figure 6 A, B. These results indicated that miR-490-5p targeted c-FOS and negatively regulated its expression in bladder cancer.


MicroRNA-490-5p is a novel tumor suppressor targeting c-FOS in human bladder cancer.

Lan G, Yang L, Xie X, Peng L, Wang Y - Arch Med Sci (2015)

Effects of miR-490-5p overexpression on c-FOS expression in T24 cells. Quantitative results of western blotting are shown in (A) and representative western blotting results are shown in (B). UVRAG was also blotted and served as an internal control
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4495152&req=5

Figure 0005: Effects of miR-490-5p overexpression on c-FOS expression in T24 cells. Quantitative results of western blotting are shown in (A) and representative western blotting results are shown in (B). UVRAG was also blotted and served as an internal control
Mentions: We further investigated the effect of miR-490-5p overexpression on the level of c-FOS. Quantitative results of western blotting are shown in Figure 5 A, and representative western blotting results are shown in Figure 5 B. The c-FOS protein level in the miR-490-5p mimics group was significantly lower than that in the other two groups. The effect of miR-490-5p suppression on the level of c-FOS was also observed. The c-FOS protein level in the miR-490-5p inhibitor group was significantly higher than those in the other two groups, as shown in Figure 6 A, B. These results indicated that miR-490-5p targeted c-FOS and negatively regulated its expression in bladder cancer.

Bottom Line: We found that overexpression of miR-490-5p in T24 cells could inhibit cell proliferation and invasion and induce cell apoptosis.In addition, our bioinformatics prediction and experimental data showed that c-FOS was a potential target of miR-490-5p.The expression level of c-FOS was significantly decreased after miR-490-5p overexpression and significantly increased after miR-490-5p suppression, indicating that c-FOS was a target of miR-490-5p.

View Article: PubMed Central - PubMed

Affiliation: Department of Kidney Transplantation, The Second Xiangya Hospital of Central South University, Changsha, China.

ABSTRACT

Introduction: Recent studies have demonstrated the critical roles of micro-RNAs in tumorigenesis and tumor progression. Here, we describe the regulation and function of miR-490-5p in bladder cancer.

Material and methods: Paired tissue samples were collected from bladder cancer patients (n = 20). Real-time PCR revealed that miR-490-5p expression was significantly down-regulated in human bladder cancer tissues and cells. Also there was an inverse relationship between the expression level of miR-490-5p and the pathological grade of bladder cancer. Western blotting was performed to detect the expression levels of c-FOS and TET1 in 6 matched tumor tissue samples and 4 bladder cell lines. Furthermore, to better understand the underlying mechanisms of miR-490-5p, we conducted gain and loss of function analysis by transfecting bladder cancer T24 cells with chemically synthesized miR-490-5p mimics and inhibitor, respectively.

Results: We found that overexpression of miR-490-5p in T24 cells could inhibit cell proliferation and invasion and induce cell apoptosis. Conversely, suppression of miR-490-5p expression induced cell proliferation and invasion, while it inhibited cell apoptosis. In addition, our bioinformatics prediction and experimental data showed that c-FOS was a potential target of miR-490-5p. The expression level of c-FOS was significantly decreased after miR-490-5p overexpression and significantly increased after miR-490-5p suppression, indicating that c-FOS was a target of miR-490-5p.

Conclusions: These findings suggest that miR-490-5p is a novel tumor suppressor, contributing to the carcinogenesis of bladder cancer by targeting c-FOS.

No MeSH data available.


Related in: MedlinePlus