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MicroRNA-490-5p is a novel tumor suppressor targeting c-FOS in human bladder cancer.

Lan G, Yang L, Xie X, Peng L, Wang Y - Arch Med Sci (2015)

Bottom Line: We found that overexpression of miR-490-5p in T24 cells could inhibit cell proliferation and invasion and induce cell apoptosis.In addition, our bioinformatics prediction and experimental data showed that c-FOS was a potential target of miR-490-5p.The expression level of c-FOS was significantly decreased after miR-490-5p overexpression and significantly increased after miR-490-5p suppression, indicating that c-FOS was a target of miR-490-5p.

View Article: PubMed Central - PubMed

Affiliation: Department of Kidney Transplantation, The Second Xiangya Hospital of Central South University, Changsha, China.

ABSTRACT

Introduction: Recent studies have demonstrated the critical roles of micro-RNAs in tumorigenesis and tumor progression. Here, we describe the regulation and function of miR-490-5p in bladder cancer.

Material and methods: Paired tissue samples were collected from bladder cancer patients (n = 20). Real-time PCR revealed that miR-490-5p expression was significantly down-regulated in human bladder cancer tissues and cells. Also there was an inverse relationship between the expression level of miR-490-5p and the pathological grade of bladder cancer. Western blotting was performed to detect the expression levels of c-FOS and TET1 in 6 matched tumor tissue samples and 4 bladder cell lines. Furthermore, to better understand the underlying mechanisms of miR-490-5p, we conducted gain and loss of function analysis by transfecting bladder cancer T24 cells with chemically synthesized miR-490-5p mimics and inhibitor, respectively.

Results: We found that overexpression of miR-490-5p in T24 cells could inhibit cell proliferation and invasion and induce cell apoptosis. Conversely, suppression of miR-490-5p expression induced cell proliferation and invasion, while it inhibited cell apoptosis. In addition, our bioinformatics prediction and experimental data showed that c-FOS was a potential target of miR-490-5p. The expression level of c-FOS was significantly decreased after miR-490-5p overexpression and significantly increased after miR-490-5p suppression, indicating that c-FOS was a target of miR-490-5p.

Conclusions: These findings suggest that miR-490-5p is a novel tumor suppressor, contributing to the carcinogenesis of bladder cancer by targeting c-FOS.

No MeSH data available.


Related in: MedlinePlus

Effects of miR-490-5p suppression on cell proliferation, apoptosis and invasion activity in T24 cell line in vitro. A – Expression level of miR-490-5p in the untreated group, the negative control miRNA group and the miR-490-5p inhibitor group (*p < 0.01). B – Cell viability assayed by MTT assay at 0 h, 24 h, 48 h and 72 h after transfection (*p < 0.05 at 48 h; ** p < 0.01 at 72 h). C – Caspase-3 level in T24 cells after miR-490-5p suppression (*p < 0.01). D – Cell invasion ability after miR-490-5p suppression in T24 cells (*p < 0.01)
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Figure 0004: Effects of miR-490-5p suppression on cell proliferation, apoptosis and invasion activity in T24 cell line in vitro. A – Expression level of miR-490-5p in the untreated group, the negative control miRNA group and the miR-490-5p inhibitor group (*p < 0.01). B – Cell viability assayed by MTT assay at 0 h, 24 h, 48 h and 72 h after transfection (*p < 0.05 at 48 h; ** p < 0.01 at 72 h). C – Caspase-3 level in T24 cells after miR-490-5p suppression (*p < 0.01). D – Cell invasion ability after miR-490-5p suppression in T24 cells (*p < 0.01)

Mentions: We transfected T24 cells with miR-490-5p inhibitor and evaluated the effects of miR-490-5p suppression. As shown in Figure 4 A, the expression level of miR-490-5p in the miR-490-5p inhibitor group was significantly lower than in the negative control miRNA group and the untreated group, indicating that the miR-490-5p inhibitor was successfully transfected into the T24 cells.


MicroRNA-490-5p is a novel tumor suppressor targeting c-FOS in human bladder cancer.

Lan G, Yang L, Xie X, Peng L, Wang Y - Arch Med Sci (2015)

Effects of miR-490-5p suppression on cell proliferation, apoptosis and invasion activity in T24 cell line in vitro. A – Expression level of miR-490-5p in the untreated group, the negative control miRNA group and the miR-490-5p inhibitor group (*p < 0.01). B – Cell viability assayed by MTT assay at 0 h, 24 h, 48 h and 72 h after transfection (*p < 0.05 at 48 h; ** p < 0.01 at 72 h). C – Caspase-3 level in T24 cells after miR-490-5p suppression (*p < 0.01). D – Cell invasion ability after miR-490-5p suppression in T24 cells (*p < 0.01)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4495152&req=5

Figure 0004: Effects of miR-490-5p suppression on cell proliferation, apoptosis and invasion activity in T24 cell line in vitro. A – Expression level of miR-490-5p in the untreated group, the negative control miRNA group and the miR-490-5p inhibitor group (*p < 0.01). B – Cell viability assayed by MTT assay at 0 h, 24 h, 48 h and 72 h after transfection (*p < 0.05 at 48 h; ** p < 0.01 at 72 h). C – Caspase-3 level in T24 cells after miR-490-5p suppression (*p < 0.01). D – Cell invasion ability after miR-490-5p suppression in T24 cells (*p < 0.01)
Mentions: We transfected T24 cells with miR-490-5p inhibitor and evaluated the effects of miR-490-5p suppression. As shown in Figure 4 A, the expression level of miR-490-5p in the miR-490-5p inhibitor group was significantly lower than in the negative control miRNA group and the untreated group, indicating that the miR-490-5p inhibitor was successfully transfected into the T24 cells.

Bottom Line: We found that overexpression of miR-490-5p in T24 cells could inhibit cell proliferation and invasion and induce cell apoptosis.In addition, our bioinformatics prediction and experimental data showed that c-FOS was a potential target of miR-490-5p.The expression level of c-FOS was significantly decreased after miR-490-5p overexpression and significantly increased after miR-490-5p suppression, indicating that c-FOS was a target of miR-490-5p.

View Article: PubMed Central - PubMed

Affiliation: Department of Kidney Transplantation, The Second Xiangya Hospital of Central South University, Changsha, China.

ABSTRACT

Introduction: Recent studies have demonstrated the critical roles of micro-RNAs in tumorigenesis and tumor progression. Here, we describe the regulation and function of miR-490-5p in bladder cancer.

Material and methods: Paired tissue samples were collected from bladder cancer patients (n = 20). Real-time PCR revealed that miR-490-5p expression was significantly down-regulated in human bladder cancer tissues and cells. Also there was an inverse relationship between the expression level of miR-490-5p and the pathological grade of bladder cancer. Western blotting was performed to detect the expression levels of c-FOS and TET1 in 6 matched tumor tissue samples and 4 bladder cell lines. Furthermore, to better understand the underlying mechanisms of miR-490-5p, we conducted gain and loss of function analysis by transfecting bladder cancer T24 cells with chemically synthesized miR-490-5p mimics and inhibitor, respectively.

Results: We found that overexpression of miR-490-5p in T24 cells could inhibit cell proliferation and invasion and induce cell apoptosis. Conversely, suppression of miR-490-5p expression induced cell proliferation and invasion, while it inhibited cell apoptosis. In addition, our bioinformatics prediction and experimental data showed that c-FOS was a potential target of miR-490-5p. The expression level of c-FOS was significantly decreased after miR-490-5p overexpression and significantly increased after miR-490-5p suppression, indicating that c-FOS was a target of miR-490-5p.

Conclusions: These findings suggest that miR-490-5p is a novel tumor suppressor, contributing to the carcinogenesis of bladder cancer by targeting c-FOS.

No MeSH data available.


Related in: MedlinePlus