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MicroRNA-490-5p is a novel tumor suppressor targeting c-FOS in human bladder cancer.

Lan G, Yang L, Xie X, Peng L, Wang Y - Arch Med Sci (2015)

Bottom Line: We found that overexpression of miR-490-5p in T24 cells could inhibit cell proliferation and invasion and induce cell apoptosis.In addition, our bioinformatics prediction and experimental data showed that c-FOS was a potential target of miR-490-5p.The expression level of c-FOS was significantly decreased after miR-490-5p overexpression and significantly increased after miR-490-5p suppression, indicating that c-FOS was a target of miR-490-5p.

View Article: PubMed Central - PubMed

Affiliation: Department of Kidney Transplantation, The Second Xiangya Hospital of Central South University, Changsha, China.

ABSTRACT

Introduction: Recent studies have demonstrated the critical roles of micro-RNAs in tumorigenesis and tumor progression. Here, we describe the regulation and function of miR-490-5p in bladder cancer.

Material and methods: Paired tissue samples were collected from bladder cancer patients (n = 20). Real-time PCR revealed that miR-490-5p expression was significantly down-regulated in human bladder cancer tissues and cells. Also there was an inverse relationship between the expression level of miR-490-5p and the pathological grade of bladder cancer. Western blotting was performed to detect the expression levels of c-FOS and TET1 in 6 matched tumor tissue samples and 4 bladder cell lines. Furthermore, to better understand the underlying mechanisms of miR-490-5p, we conducted gain and loss of function analysis by transfecting bladder cancer T24 cells with chemically synthesized miR-490-5p mimics and inhibitor, respectively.

Results: We found that overexpression of miR-490-5p in T24 cells could inhibit cell proliferation and invasion and induce cell apoptosis. Conversely, suppression of miR-490-5p expression induced cell proliferation and invasion, while it inhibited cell apoptosis. In addition, our bioinformatics prediction and experimental data showed that c-FOS was a potential target of miR-490-5p. The expression level of c-FOS was significantly decreased after miR-490-5p overexpression and significantly increased after miR-490-5p suppression, indicating that c-FOS was a target of miR-490-5p.

Conclusions: These findings suggest that miR-490-5p is a novel tumor suppressor, contributing to the carcinogenesis of bladder cancer by targeting c-FOS.

No MeSH data available.


Related in: MedlinePlus

Relative expression levels of miR-490-5p in bladder cancer tissues and bladder cancer cell lines. Expression levels of miR-490-5p were examined by real-time PCR. A – Expression levels of miR-490-5p in SV-HUC-1 cells and three bladder cancer cell lines. One-way LSD test, *p < 0.01. B – Expression levels of miR-490-5p in 20 paired bladder cancer and adjacent non-tumor tissues. Student's t-test, *p < 0.01. C – Expression levels of miR-490-5p in different pathological grades. Student's t-test, *p < 0.01T – Tumor tissues, N – adjacent non-tumor tissues.
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Figure 0001: Relative expression levels of miR-490-5p in bladder cancer tissues and bladder cancer cell lines. Expression levels of miR-490-5p were examined by real-time PCR. A – Expression levels of miR-490-5p in SV-HUC-1 cells and three bladder cancer cell lines. One-way LSD test, *p < 0.01. B – Expression levels of miR-490-5p in 20 paired bladder cancer and adjacent non-tumor tissues. Student's t-test, *p < 0.01. C – Expression levels of miR-490-5p in different pathological grades. Student's t-test, *p < 0.01T – Tumor tissues, N – adjacent non-tumor tissues.

Mentions: First, we analyzed the expression levels of miR-490-5p in three human bladder cancer cell lines by real-time PCR. As shown in Figure 1 A, the miR-490-5p expression levels in three bladder cancer cell lines were significantly down-regulated compared with those in SV-HUC-1 cells. Next we detected the expression levels of miR-490-5p in 20 bladder cancer tissues and their paired adjacent non-tumor tissues. Consistent with the data obtained from bladder cancer cell lines, the average expression level of miR-490-5p was significantly lower in bladder cancer tissues than in paired adjacent non-tumor tissues (Figure 1 B).


MicroRNA-490-5p is a novel tumor suppressor targeting c-FOS in human bladder cancer.

Lan G, Yang L, Xie X, Peng L, Wang Y - Arch Med Sci (2015)

Relative expression levels of miR-490-5p in bladder cancer tissues and bladder cancer cell lines. Expression levels of miR-490-5p were examined by real-time PCR. A – Expression levels of miR-490-5p in SV-HUC-1 cells and three bladder cancer cell lines. One-way LSD test, *p < 0.01. B – Expression levels of miR-490-5p in 20 paired bladder cancer and adjacent non-tumor tissues. Student's t-test, *p < 0.01. C – Expression levels of miR-490-5p in different pathological grades. Student's t-test, *p < 0.01T – Tumor tissues, N – adjacent non-tumor tissues.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4495152&req=5

Figure 0001: Relative expression levels of miR-490-5p in bladder cancer tissues and bladder cancer cell lines. Expression levels of miR-490-5p were examined by real-time PCR. A – Expression levels of miR-490-5p in SV-HUC-1 cells and three bladder cancer cell lines. One-way LSD test, *p < 0.01. B – Expression levels of miR-490-5p in 20 paired bladder cancer and adjacent non-tumor tissues. Student's t-test, *p < 0.01. C – Expression levels of miR-490-5p in different pathological grades. Student's t-test, *p < 0.01T – Tumor tissues, N – adjacent non-tumor tissues.
Mentions: First, we analyzed the expression levels of miR-490-5p in three human bladder cancer cell lines by real-time PCR. As shown in Figure 1 A, the miR-490-5p expression levels in three bladder cancer cell lines were significantly down-regulated compared with those in SV-HUC-1 cells. Next we detected the expression levels of miR-490-5p in 20 bladder cancer tissues and their paired adjacent non-tumor tissues. Consistent with the data obtained from bladder cancer cell lines, the average expression level of miR-490-5p was significantly lower in bladder cancer tissues than in paired adjacent non-tumor tissues (Figure 1 B).

Bottom Line: We found that overexpression of miR-490-5p in T24 cells could inhibit cell proliferation and invasion and induce cell apoptosis.In addition, our bioinformatics prediction and experimental data showed that c-FOS was a potential target of miR-490-5p.The expression level of c-FOS was significantly decreased after miR-490-5p overexpression and significantly increased after miR-490-5p suppression, indicating that c-FOS was a target of miR-490-5p.

View Article: PubMed Central - PubMed

Affiliation: Department of Kidney Transplantation, The Second Xiangya Hospital of Central South University, Changsha, China.

ABSTRACT

Introduction: Recent studies have demonstrated the critical roles of micro-RNAs in tumorigenesis and tumor progression. Here, we describe the regulation and function of miR-490-5p in bladder cancer.

Material and methods: Paired tissue samples were collected from bladder cancer patients (n = 20). Real-time PCR revealed that miR-490-5p expression was significantly down-regulated in human bladder cancer tissues and cells. Also there was an inverse relationship between the expression level of miR-490-5p and the pathological grade of bladder cancer. Western blotting was performed to detect the expression levels of c-FOS and TET1 in 6 matched tumor tissue samples and 4 bladder cell lines. Furthermore, to better understand the underlying mechanisms of miR-490-5p, we conducted gain and loss of function analysis by transfecting bladder cancer T24 cells with chemically synthesized miR-490-5p mimics and inhibitor, respectively.

Results: We found that overexpression of miR-490-5p in T24 cells could inhibit cell proliferation and invasion and induce cell apoptosis. Conversely, suppression of miR-490-5p expression induced cell proliferation and invasion, while it inhibited cell apoptosis. In addition, our bioinformatics prediction and experimental data showed that c-FOS was a potential target of miR-490-5p. The expression level of c-FOS was significantly decreased after miR-490-5p overexpression and significantly increased after miR-490-5p suppression, indicating that c-FOS was a target of miR-490-5p.

Conclusions: These findings suggest that miR-490-5p is a novel tumor suppressor, contributing to the carcinogenesis of bladder cancer by targeting c-FOS.

No MeSH data available.


Related in: MedlinePlus