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Effects of thyroid hormones on the antioxidative status in the uterus of young adult rats.

Kong L, Wei Q, Fedail JS, Shi F, Nagaoka K, Watanabe G - J. Reprod. Dev. (2015)

Bottom Line: The results showed the body weights decreased significantly in both the hypo-T and hyper-T groups and that uterine weights were decreased significantly in the hypo-T group.The serum concentrations of total triiodothyronine (T3) and thyroxine (T4), as well as estradiol (E2), were significantly decreased in the hypo-T group, but increased in the hyper-T group.Moreover, the activities of both glutathione peroxidase (GSH-Px) and catalase (CAT) exhibited significant decreases and increases in the hypo-T and hyper-T groups, respectively.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.

ABSTRACT
Thyroid hormones and oxidative stress play significant roles in the normal functioning of the female reproductive system. Nitric oxide (NO), a free radical synthesized by nitric oxide synthases (NOS), participates in the regulation of thyroid function and is also a good biomarker for assessment of the oxidative stress status. Therefore, the purpose of this study was to investigate effects of thyroid hormones on uterine antioxidative status in young adult rats. Thirty immature female Sprague-Dawley rats were randomly divided into three groups: control, hypothyroid (hypo-T) and hyperthyroid (hyper-T). The results showed the body weights decreased significantly in both the hypo-T and hyper-T groups and that uterine weights were decreased significantly in the hypo-T group. The serum concentrations of total triiodothyronine (T3) and thyroxine (T4), as well as estradiol (E2), were significantly decreased in the hypo-T group, but increased in the hyper-T group. The progesterone (P4) concentrations in the hypo- and hyperthyroid rats markedly decreased. Immunohistochemistry results provided evidence that thyroid hormone nuclear receptor α/β (TRα/β) and three NOS isoforms were located in different cell types of rat uteri. The NO content and total NOS and inducible NOS (iNOS) activities were markedly diminished in the hypo-T group but increased in the hyper-T group. Moreover, the activities of both glutathione peroxidase (GSH-Px) and catalase (CAT) exhibited significant decreases and increases in the hypo-T and hyper-T groups, respectively. The malondialdehyde (MDA) contents in both the hypo-T and hyper-T groups showed a significant increase. Total superoxide dismutase (T-SOD) activity in the hypo- and hyper-T rats markedly decreased. In conclusion, these results indicated that thyroid hormones have an important influence on the modulation of uterine antioxidative status.

No MeSH data available.


Related in: MedlinePlus

Immunostaining of TRα/β and the three NOS isoforms (nNOS, iNOS and eNOS) in rat uteri. The staining intensities of TRα/β (A1-3), nNOS (B1-3), iNOS (C1-3) and eNOS (D1-3) were qualitatively different in various cell types of rat uterine. No specific staining was observed in the negative control sections (A4, B4, C4 and D4). NC, negative control sections; LEC, luminal epithelial cells; GEC, glandular epithelial cells; SC, stromal cells; MC, myometrial cells. Scale bar =100 µm.
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fig_003: Immunostaining of TRα/β and the three NOS isoforms (nNOS, iNOS and eNOS) in rat uteri. The staining intensities of TRα/β (A1-3), nNOS (B1-3), iNOS (C1-3) and eNOS (D1-3) were qualitatively different in various cell types of rat uterine. No specific staining was observed in the negative control sections (A4, B4, C4 and D4). NC, negative control sections; LEC, luminal epithelial cells; GEC, glandular epithelial cells; SC, stromal cells; MC, myometrial cells. Scale bar =100 µm.

Mentions: From the results of immunohistochemical staining, TRα/β was expressed more strongly in the uterine luminal epithelium and endometrial gland epithelium cells compared with other areas of rat uteri; as a result, the intensity of immunostaining was weak in myometrial smooth muscle cells, and immunostaining was not detected in stromal cells. The relative levels of immunostaining intensity in each treated group were different from those of the control group. The immunostaining intensity of TRα/β in the hyper-T group was increased, while it was decreased in the hypo-T group (Fig. 3-A1, -A2 and -A3Fig. 3.


Effects of thyroid hormones on the antioxidative status in the uterus of young adult rats.

Kong L, Wei Q, Fedail JS, Shi F, Nagaoka K, Watanabe G - J. Reprod. Dev. (2015)

Immunostaining of TRα/β and the three NOS isoforms (nNOS, iNOS and eNOS) in rat uteri. The staining intensities of TRα/β (A1-3), nNOS (B1-3), iNOS (C1-3) and eNOS (D1-3) were qualitatively different in various cell types of rat uterine. No specific staining was observed in the negative control sections (A4, B4, C4 and D4). NC, negative control sections; LEC, luminal epithelial cells; GEC, glandular epithelial cells; SC, stromal cells; MC, myometrial cells. Scale bar =100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4495068&req=5

fig_003: Immunostaining of TRα/β and the three NOS isoforms (nNOS, iNOS and eNOS) in rat uteri. The staining intensities of TRα/β (A1-3), nNOS (B1-3), iNOS (C1-3) and eNOS (D1-3) were qualitatively different in various cell types of rat uterine. No specific staining was observed in the negative control sections (A4, B4, C4 and D4). NC, negative control sections; LEC, luminal epithelial cells; GEC, glandular epithelial cells; SC, stromal cells; MC, myometrial cells. Scale bar =100 µm.
Mentions: From the results of immunohistochemical staining, TRα/β was expressed more strongly in the uterine luminal epithelium and endometrial gland epithelium cells compared with other areas of rat uteri; as a result, the intensity of immunostaining was weak in myometrial smooth muscle cells, and immunostaining was not detected in stromal cells. The relative levels of immunostaining intensity in each treated group were different from those of the control group. The immunostaining intensity of TRα/β in the hyper-T group was increased, while it was decreased in the hypo-T group (Fig. 3-A1, -A2 and -A3Fig. 3.

Bottom Line: The results showed the body weights decreased significantly in both the hypo-T and hyper-T groups and that uterine weights were decreased significantly in the hypo-T group.The serum concentrations of total triiodothyronine (T3) and thyroxine (T4), as well as estradiol (E2), were significantly decreased in the hypo-T group, but increased in the hyper-T group.Moreover, the activities of both glutathione peroxidase (GSH-Px) and catalase (CAT) exhibited significant decreases and increases in the hypo-T and hyper-T groups, respectively.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.

ABSTRACT
Thyroid hormones and oxidative stress play significant roles in the normal functioning of the female reproductive system. Nitric oxide (NO), a free radical synthesized by nitric oxide synthases (NOS), participates in the regulation of thyroid function and is also a good biomarker for assessment of the oxidative stress status. Therefore, the purpose of this study was to investigate effects of thyroid hormones on uterine antioxidative status in young adult rats. Thirty immature female Sprague-Dawley rats were randomly divided into three groups: control, hypothyroid (hypo-T) and hyperthyroid (hyper-T). The results showed the body weights decreased significantly in both the hypo-T and hyper-T groups and that uterine weights were decreased significantly in the hypo-T group. The serum concentrations of total triiodothyronine (T3) and thyroxine (T4), as well as estradiol (E2), were significantly decreased in the hypo-T group, but increased in the hyper-T group. The progesterone (P4) concentrations in the hypo- and hyperthyroid rats markedly decreased. Immunohistochemistry results provided evidence that thyroid hormone nuclear receptor α/β (TRα/β) and three NOS isoforms were located in different cell types of rat uteri. The NO content and total NOS and inducible NOS (iNOS) activities were markedly diminished in the hypo-T group but increased in the hyper-T group. Moreover, the activities of both glutathione peroxidase (GSH-Px) and catalase (CAT) exhibited significant decreases and increases in the hypo-T and hyper-T groups, respectively. The malondialdehyde (MDA) contents in both the hypo-T and hyper-T groups showed a significant increase. Total superoxide dismutase (T-SOD) activity in the hypo- and hyper-T rats markedly decreased. In conclusion, these results indicated that thyroid hormones have an important influence on the modulation of uterine antioxidative status.

No MeSH data available.


Related in: MedlinePlus