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High USP22 expression indicates poor prognosis in hepatocellular carcinoma.

Tang B, Tang F, Li B, Yuan S, Xu Q, Tomlinson S, Jin J, Hu W, He S - Oncotarget (2015)

Bottom Line: Kaplan-Meier analysis showed that elevated USP22 expression predicted poorer overall survival and recurrence-free survival.Multivariate analyses revealed that USP22 expression is an independent prognostic parameter in HCC.These findings provide evidence that high USP22 expression might be important in tumor progression and serves as an independent molecular marker for poor HCC prognosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, People's Republic of China.

ABSTRACT
Ubiquitin-specific protease 22 (USP22) removes ubiquitin from histones, thus regulating gene transcription. The expression frequency and expression levels of USP22 were significantly higher in hepatocellular carcinoma (HCC) than in normal liver tissues. High USP22 expression in HCC was significantly correlated with clinical stage and tumor grade. Kaplan-Meier analysis showed that elevated USP22 expression predicted poorer overall survival and recurrence-free survival. High USP22 expression was also associated with shortened survival time in patients at advanced tumor stages and with high grade HCC. Multivariate analyses revealed that USP22 expression is an independent prognostic parameter in HCC. These findings provide evidence that high USP22 expression might be important in tumor progression and serves as an independent molecular marker for poor HCC prognosis. Thus, USP22 overexpression identifies patients at high risk and represents a novel therapeutic molecular target for this tumor.

No MeSH data available.


Related in: MedlinePlus

USP22 expression in HCC and normal liver tissues(A) Semi-quantitative RT-PCR analysis of USP22 mRNA expression in HCC specimens (T) and normal adjacent hepatic tissue (N); (B) Western blot analysis of USP22 protein expression in representative HCC (T) and normal adjacent tissue (N); (C) Quantitative RT-PCR analysis of USP22 expression in HCC specimens (T) and normal adjacent hepatic tissue (N). β-actin served as an internal control.
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Figure 2: USP22 expression in HCC and normal liver tissues(A) Semi-quantitative RT-PCR analysis of USP22 mRNA expression in HCC specimens (T) and normal adjacent hepatic tissue (N); (B) Western blot analysis of USP22 protein expression in representative HCC (T) and normal adjacent tissue (N); (C) Quantitative RT-PCR analysis of USP22 expression in HCC specimens (T) and normal adjacent hepatic tissue (N). β-actin served as an internal control.

Mentions: Based on the semi-quantitative RT-PCR analysis of seven pairs of HCC and normal adjacent liver tissues, we found that USP22 mRNA was overexpressed in HCC (Fig. 2A); this finding was confirmed using quantitative RT-PCR analysis (Fig. 2C). To further confirm that USP22 is overexpressed in HCC, the protein levels of USP22 in HCC and matching normal adjacent liver tissues were analyzed using Western blots. As shown in Fig. 2B, the USP22 protein was overexpressed in HCC specimens but not in the matched normal adjacent liver tissues.


High USP22 expression indicates poor prognosis in hepatocellular carcinoma.

Tang B, Tang F, Li B, Yuan S, Xu Q, Tomlinson S, Jin J, Hu W, He S - Oncotarget (2015)

USP22 expression in HCC and normal liver tissues(A) Semi-quantitative RT-PCR analysis of USP22 mRNA expression in HCC specimens (T) and normal adjacent hepatic tissue (N); (B) Western blot analysis of USP22 protein expression in representative HCC (T) and normal adjacent tissue (N); (C) Quantitative RT-PCR analysis of USP22 expression in HCC specimens (T) and normal adjacent hepatic tissue (N). β-actin served as an internal control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494964&req=5

Figure 2: USP22 expression in HCC and normal liver tissues(A) Semi-quantitative RT-PCR analysis of USP22 mRNA expression in HCC specimens (T) and normal adjacent hepatic tissue (N); (B) Western blot analysis of USP22 protein expression in representative HCC (T) and normal adjacent tissue (N); (C) Quantitative RT-PCR analysis of USP22 expression in HCC specimens (T) and normal adjacent hepatic tissue (N). β-actin served as an internal control.
Mentions: Based on the semi-quantitative RT-PCR analysis of seven pairs of HCC and normal adjacent liver tissues, we found that USP22 mRNA was overexpressed in HCC (Fig. 2A); this finding was confirmed using quantitative RT-PCR analysis (Fig. 2C). To further confirm that USP22 is overexpressed in HCC, the protein levels of USP22 in HCC and matching normal adjacent liver tissues were analyzed using Western blots. As shown in Fig. 2B, the USP22 protein was overexpressed in HCC specimens but not in the matched normal adjacent liver tissues.

Bottom Line: Kaplan-Meier analysis showed that elevated USP22 expression predicted poorer overall survival and recurrence-free survival.Multivariate analyses revealed that USP22 expression is an independent prognostic parameter in HCC.These findings provide evidence that high USP22 expression might be important in tumor progression and serves as an independent molecular marker for poor HCC prognosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, People's Republic of China.

ABSTRACT
Ubiquitin-specific protease 22 (USP22) removes ubiquitin from histones, thus regulating gene transcription. The expression frequency and expression levels of USP22 were significantly higher in hepatocellular carcinoma (HCC) than in normal liver tissues. High USP22 expression in HCC was significantly correlated with clinical stage and tumor grade. Kaplan-Meier analysis showed that elevated USP22 expression predicted poorer overall survival and recurrence-free survival. High USP22 expression was also associated with shortened survival time in patients at advanced tumor stages and with high grade HCC. Multivariate analyses revealed that USP22 expression is an independent prognostic parameter in HCC. These findings provide evidence that high USP22 expression might be important in tumor progression and serves as an independent molecular marker for poor HCC prognosis. Thus, USP22 overexpression identifies patients at high risk and represents a novel therapeutic molecular target for this tumor.

No MeSH data available.


Related in: MedlinePlus