Limits...
High USP22 expression indicates poor prognosis in hepatocellular carcinoma.

Tang B, Tang F, Li B, Yuan S, Xu Q, Tomlinson S, Jin J, Hu W, He S - Oncotarget (2015)

Bottom Line: Kaplan-Meier analysis showed that elevated USP22 expression predicted poorer overall survival and recurrence-free survival.Multivariate analyses revealed that USP22 expression is an independent prognostic parameter in HCC.These findings provide evidence that high USP22 expression might be important in tumor progression and serves as an independent molecular marker for poor HCC prognosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, People's Republic of China.

ABSTRACT
Ubiquitin-specific protease 22 (USP22) removes ubiquitin from histones, thus regulating gene transcription. The expression frequency and expression levels of USP22 were significantly higher in hepatocellular carcinoma (HCC) than in normal liver tissues. High USP22 expression in HCC was significantly correlated with clinical stage and tumor grade. Kaplan-Meier analysis showed that elevated USP22 expression predicted poorer overall survival and recurrence-free survival. High USP22 expression was also associated with shortened survival time in patients at advanced tumor stages and with high grade HCC. Multivariate analyses revealed that USP22 expression is an independent prognostic parameter in HCC. These findings provide evidence that high USP22 expression might be important in tumor progression and serves as an independent molecular marker for poor HCC prognosis. Thus, USP22 overexpression identifies patients at high risk and represents a novel therapeutic molecular target for this tumor.

No MeSH data available.


Related in: MedlinePlus

USP22 expression in HCC tissues and cell lines(A) Semi-quantitative RT-PCR analysis of USP22 mRNA expression in seven HCC cell lines (Bel-7402, HepG2, SK-Hep-1, HuH-7, Hep3B, QGY-7701, and SMMC-7721) and a normal hepatic cell line (LO2); (B) Western blot analysis of USP22 protein expression in seven HCC cell lines (Bel-7402, HepG2, SK-Hep-1, HuH-7, Hep3B, QGY-7701, and SMMC-7721) and a normal hepatic cell line (LO2). β-actin was used as internal control for RT-PCR and Western blot.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4494964&req=5

Figure 1: USP22 expression in HCC tissues and cell lines(A) Semi-quantitative RT-PCR analysis of USP22 mRNA expression in seven HCC cell lines (Bel-7402, HepG2, SK-Hep-1, HuH-7, Hep3B, QGY-7701, and SMMC-7721) and a normal hepatic cell line (LO2); (B) Western blot analysis of USP22 protein expression in seven HCC cell lines (Bel-7402, HepG2, SK-Hep-1, HuH-7, Hep3B, QGY-7701, and SMMC-7721) and a normal hepatic cell line (LO2). β-actin was used as internal control for RT-PCR and Western blot.

Mentions: USP22 expression was analyzed in seven established human HCC cell lines (Bel-7402, HepG2, HuH-7, SK-Hep-1, Hep3B, QGY-7701 and SMMC-7721) and a normal hepatic cell line LO2 using RT-PCR and Western blot assay. HuH-1 is a well-differentiated HCC cell line, whereas Bel-7402, HepG2, SK-Hep-1, Hep3B, QGY-7701 and SMMC-7721 are moderately to poorly differentiated HCC cell lines. As shown in Fig. 1A and 1B, USP22 expression was significantly higher in the poorly and moderately differentiated cells (Bel-7402, HepG2, SK-Hep-1, Hep3B, QGY-7701 and SMMC-7721) than in the well-differentiated and normal hepatic cells (HuH-1 and LO2). These initial observations revealed that de-ubiquitination might be correlated with HCC differentiation.


High USP22 expression indicates poor prognosis in hepatocellular carcinoma.

Tang B, Tang F, Li B, Yuan S, Xu Q, Tomlinson S, Jin J, Hu W, He S - Oncotarget (2015)

USP22 expression in HCC tissues and cell lines(A) Semi-quantitative RT-PCR analysis of USP22 mRNA expression in seven HCC cell lines (Bel-7402, HepG2, SK-Hep-1, HuH-7, Hep3B, QGY-7701, and SMMC-7721) and a normal hepatic cell line (LO2); (B) Western blot analysis of USP22 protein expression in seven HCC cell lines (Bel-7402, HepG2, SK-Hep-1, HuH-7, Hep3B, QGY-7701, and SMMC-7721) and a normal hepatic cell line (LO2). β-actin was used as internal control for RT-PCR and Western blot.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494964&req=5

Figure 1: USP22 expression in HCC tissues and cell lines(A) Semi-quantitative RT-PCR analysis of USP22 mRNA expression in seven HCC cell lines (Bel-7402, HepG2, SK-Hep-1, HuH-7, Hep3B, QGY-7701, and SMMC-7721) and a normal hepatic cell line (LO2); (B) Western blot analysis of USP22 protein expression in seven HCC cell lines (Bel-7402, HepG2, SK-Hep-1, HuH-7, Hep3B, QGY-7701, and SMMC-7721) and a normal hepatic cell line (LO2). β-actin was used as internal control for RT-PCR and Western blot.
Mentions: USP22 expression was analyzed in seven established human HCC cell lines (Bel-7402, HepG2, HuH-7, SK-Hep-1, Hep3B, QGY-7701 and SMMC-7721) and a normal hepatic cell line LO2 using RT-PCR and Western blot assay. HuH-1 is a well-differentiated HCC cell line, whereas Bel-7402, HepG2, SK-Hep-1, Hep3B, QGY-7701 and SMMC-7721 are moderately to poorly differentiated HCC cell lines. As shown in Fig. 1A and 1B, USP22 expression was significantly higher in the poorly and moderately differentiated cells (Bel-7402, HepG2, SK-Hep-1, Hep3B, QGY-7701 and SMMC-7721) than in the well-differentiated and normal hepatic cells (HuH-1 and LO2). These initial observations revealed that de-ubiquitination might be correlated with HCC differentiation.

Bottom Line: Kaplan-Meier analysis showed that elevated USP22 expression predicted poorer overall survival and recurrence-free survival.Multivariate analyses revealed that USP22 expression is an independent prognostic parameter in HCC.These findings provide evidence that high USP22 expression might be important in tumor progression and serves as an independent molecular marker for poor HCC prognosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, People's Republic of China.

ABSTRACT
Ubiquitin-specific protease 22 (USP22) removes ubiquitin from histones, thus regulating gene transcription. The expression frequency and expression levels of USP22 were significantly higher in hepatocellular carcinoma (HCC) than in normal liver tissues. High USP22 expression in HCC was significantly correlated with clinical stage and tumor grade. Kaplan-Meier analysis showed that elevated USP22 expression predicted poorer overall survival and recurrence-free survival. High USP22 expression was also associated with shortened survival time in patients at advanced tumor stages and with high grade HCC. Multivariate analyses revealed that USP22 expression is an independent prognostic parameter in HCC. These findings provide evidence that high USP22 expression might be important in tumor progression and serves as an independent molecular marker for poor HCC prognosis. Thus, USP22 overexpression identifies patients at high risk and represents a novel therapeutic molecular target for this tumor.

No MeSH data available.


Related in: MedlinePlus