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PAQR3 expression is downregulated in human breast cancers and correlated with HER2 expression.

Li Z, Ling ZQ, Guo W, Lu XX, Pan Y, Wang Z, Chen Y - Oncotarget (2015)

Bottom Line: Knockdown of PAQR3 in MDA-MD-231 cells elevated cell proliferation and migration.PAQR3 is able to modulate the proliferation and migration of breast cancer cells.Our data indicate that PAQR3 functions as a tumor suppressor in the development of human breast cancers.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, China.

ABSTRACT
PAQR3 is a newly discovered tumor suppressor and its functional role in breast cancer has not been well characterized. We report here that PAQR3 is associated with the progression and survival of human patients with breast cancer, as well as cell proliferation and migration of human breast cancer cells. PAQR3 mRNA level was robustly downregulated in human breast cancer samples compared with their corresponding para-cancerous histological normal tissues (n = 82, P < 0.0001). The mRNA level of PAQR3 was negatively correlated with HER2 expression (P < 0.0001) and disease-free survival of the patients (P < 0.0001). PAQR3 overexpression inhibited cell proliferation, colony formation and migration of breast cancer cells including MCF7, SKBR3, MDA-MD-231, MDA-MD-468 and MDA-MD-453 cells. Knockdown of PAQR3 in MDA-MD-231 cells elevated cell proliferation and migration. Inhibition of HER2 by trastuzumab increased PAQR3 expression in SKBR3 cells. In conclusion, PAQR3 expression is frequently downregulated in human breast cancers inversely correlated with HER2 expression. PAQR3 is able to modulate the proliferation and migration of breast cancer cells. Our data indicate that PAQR3 functions as a tumor suppressor in the development of human breast cancers.

No MeSH data available.


Related in: MedlinePlus

PAQR3 overexpression reduces cell growth and colony formation of human breast cancer cells(A) The expression level of PAQR3 in breast cancer cells were detected by quantitative RT- PCR. (B) The expression levels of PAQR3 in control and PAQR3-overexpressing cells by lentivirus infection were detected by quantitative RT-PCR. (C) MTT assay was used to determine the cell proliferation rate with the cells with or without overexpression of PAQR3 at the time point as indicated. The data are shown as mean ± SD and * for P < 0.05 and ** for P < 0.01. (D) Colony formation assay was performed with these cells. The cells were seeded into 6-well plates and cultured for 5 days, followed by crystal violet staining.
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Figure 3: PAQR3 overexpression reduces cell growth and colony formation of human breast cancer cells(A) The expression level of PAQR3 in breast cancer cells were detected by quantitative RT- PCR. (B) The expression levels of PAQR3 in control and PAQR3-overexpressing cells by lentivirus infection were detected by quantitative RT-PCR. (C) MTT assay was used to determine the cell proliferation rate with the cells with or without overexpression of PAQR3 at the time point as indicated. The data are shown as mean ± SD and * for P < 0.05 and ** for P < 0.01. (D) Colony formation assay was performed with these cells. The cells were seeded into 6-well plates and cultured for 5 days, followed by crystal violet staining.

Mentions: We next investigated whether PAQR3 has a direct effect on the growth and migration of breast cancer cells. We used five breast cancer cell lines with different status of HER2 expression. MCF7 is an ER-positive breast cancer cell line. SKBR3, MDA-MD-468 and MDA-MD-453 cell lines are HER2-positive. MDA-MD-231 cell line is triple-negative. We analyzed the PAQR3 expression levels of these cell lines (Figure 3A). Consistent with the clinical data that HER2 expression is inversely correlated with PAQR3 expression, we found that the HER2-negative MDA-MD-231 cells had the highest level of PAQR3 expression (Figure 3A).


PAQR3 expression is downregulated in human breast cancers and correlated with HER2 expression.

Li Z, Ling ZQ, Guo W, Lu XX, Pan Y, Wang Z, Chen Y - Oncotarget (2015)

PAQR3 overexpression reduces cell growth and colony formation of human breast cancer cells(A) The expression level of PAQR3 in breast cancer cells were detected by quantitative RT- PCR. (B) The expression levels of PAQR3 in control and PAQR3-overexpressing cells by lentivirus infection were detected by quantitative RT-PCR. (C) MTT assay was used to determine the cell proliferation rate with the cells with or without overexpression of PAQR3 at the time point as indicated. The data are shown as mean ± SD and * for P < 0.05 and ** for P < 0.01. (D) Colony formation assay was performed with these cells. The cells were seeded into 6-well plates and cultured for 5 days, followed by crystal violet staining.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494943&req=5

Figure 3: PAQR3 overexpression reduces cell growth and colony formation of human breast cancer cells(A) The expression level of PAQR3 in breast cancer cells were detected by quantitative RT- PCR. (B) The expression levels of PAQR3 in control and PAQR3-overexpressing cells by lentivirus infection were detected by quantitative RT-PCR. (C) MTT assay was used to determine the cell proliferation rate with the cells with or without overexpression of PAQR3 at the time point as indicated. The data are shown as mean ± SD and * for P < 0.05 and ** for P < 0.01. (D) Colony formation assay was performed with these cells. The cells were seeded into 6-well plates and cultured for 5 days, followed by crystal violet staining.
Mentions: We next investigated whether PAQR3 has a direct effect on the growth and migration of breast cancer cells. We used five breast cancer cell lines with different status of HER2 expression. MCF7 is an ER-positive breast cancer cell line. SKBR3, MDA-MD-468 and MDA-MD-453 cell lines are HER2-positive. MDA-MD-231 cell line is triple-negative. We analyzed the PAQR3 expression levels of these cell lines (Figure 3A). Consistent with the clinical data that HER2 expression is inversely correlated with PAQR3 expression, we found that the HER2-negative MDA-MD-231 cells had the highest level of PAQR3 expression (Figure 3A).

Bottom Line: Knockdown of PAQR3 in MDA-MD-231 cells elevated cell proliferation and migration.PAQR3 is able to modulate the proliferation and migration of breast cancer cells.Our data indicate that PAQR3 functions as a tumor suppressor in the development of human breast cancers.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, China.

ABSTRACT
PAQR3 is a newly discovered tumor suppressor and its functional role in breast cancer has not been well characterized. We report here that PAQR3 is associated with the progression and survival of human patients with breast cancer, as well as cell proliferation and migration of human breast cancer cells. PAQR3 mRNA level was robustly downregulated in human breast cancer samples compared with their corresponding para-cancerous histological normal tissues (n = 82, P < 0.0001). The mRNA level of PAQR3 was negatively correlated with HER2 expression (P < 0.0001) and disease-free survival of the patients (P < 0.0001). PAQR3 overexpression inhibited cell proliferation, colony formation and migration of breast cancer cells including MCF7, SKBR3, MDA-MD-231, MDA-MD-468 and MDA-MD-453 cells. Knockdown of PAQR3 in MDA-MD-231 cells elevated cell proliferation and migration. Inhibition of HER2 by trastuzumab increased PAQR3 expression in SKBR3 cells. In conclusion, PAQR3 expression is frequently downregulated in human breast cancers inversely correlated with HER2 expression. PAQR3 is able to modulate the proliferation and migration of breast cancer cells. Our data indicate that PAQR3 functions as a tumor suppressor in the development of human breast cancers.

No MeSH data available.


Related in: MedlinePlus