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miR-20b is up-regulated in brain metastases from primary breast cancers.

Ahmad A, Ginnebaugh KR, Sethi S, Chen W, Ali R, Mittal S, Sarkar FH - Oncotarget (2015)

Bottom Line: However, unique molecular biomarkers have not yet been established.We further tested the effect of miR-20b overexpression on colony formation and invasion in vitro using MCF-7 and MDA-MB-231 cells.Further, miR-20b levels were observed to be high in brain-metastasizing cells, compared to bone-metastasizing cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Wayne State University School of Medicine and Karmanos Cancer Institute, Detroit, Michigan, USA.

ABSTRACT
Brain metastases are frequent in patients with advanced breast cancer and are associated with poor prognosis. However, unique molecular biomarkers have not yet been established. We hypothesized that microRNA-20b (miR-20b) plays a role in breast cancer brain metastasis. Our study cohort comprised of eleven breast cancer patients with brain metastasis and nine control patients (age, stage, and follow-up matched) with breast cancer without brain metastasis. Cases were reviewed microscopically to select tumor blocks with >50% tumor cells, RNA was extracted from formalin-fixed paraffin-embedded (FFPE) tumor tissue blocks and expression of miR-20b analyzed using qRT-PCR. We further tested the effect of miR-20b overexpression on colony formation and invasion in vitro using MCF-7 and MDA-MB-231 cells. In the patient-derived samples, miR-20b expression was significantly higher in brain metastases of breast cancer patients, compared to primary breast tumors as well as the patients without brain metastasis. miR-20b also significantly induced the colony formation and invasiveness of breast cancer cells. Further, miR-20b levels were observed to be high in brain-metastasizing cells, compared to bone-metastasizing cells. Together, our findings suggest a novel role of miR-20b in breast cancer brain metastasis that warrants further investigation for its potential to be developed as prognostic and/or therapeutic target.

No MeSH data available.


Related in: MedlinePlus

Patient with solitary breast cancer brain metastasis involving the posterior aspect of the left middle frontal gyrusPost-contrast T1-weighted axial (A), coronal (C), and sagittal (D) images and fluid-attenuated inversion recovery (FLAIR) axial image (B) showing a 2.5 × 3.3 × 3.1 cm mass (white arrows) with extensive peritumoral vasogenic edema (yellow arrows). Of note, this patient had the highest expression of miR-20b in the resected tumor specimen.
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Figure 1: Patient with solitary breast cancer brain metastasis involving the posterior aspect of the left middle frontal gyrusPost-contrast T1-weighted axial (A), coronal (C), and sagittal (D) images and fluid-attenuated inversion recovery (FLAIR) axial image (B) showing a 2.5 × 3.3 × 3.1 cm mass (white arrows) with extensive peritumoral vasogenic edema (yellow arrows). Of note, this patient had the highest expression of miR-20b in the resected tumor specimen.

Mentions: A total of 20 breast cancer cases met the study criteria. These included 11 breast cancer cases with brain metastasis and 9 age, stage and follow-up matched breast cancer cases without brain metastasis [12]. Figure 1 shows the typical radiographic features of a patient who underwent resection of a solitary breast cancer brain metastasis.


miR-20b is up-regulated in brain metastases from primary breast cancers.

Ahmad A, Ginnebaugh KR, Sethi S, Chen W, Ali R, Mittal S, Sarkar FH - Oncotarget (2015)

Patient with solitary breast cancer brain metastasis involving the posterior aspect of the left middle frontal gyrusPost-contrast T1-weighted axial (A), coronal (C), and sagittal (D) images and fluid-attenuated inversion recovery (FLAIR) axial image (B) showing a 2.5 × 3.3 × 3.1 cm mass (white arrows) with extensive peritumoral vasogenic edema (yellow arrows). Of note, this patient had the highest expression of miR-20b in the resected tumor specimen.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494931&req=5

Figure 1: Patient with solitary breast cancer brain metastasis involving the posterior aspect of the left middle frontal gyrusPost-contrast T1-weighted axial (A), coronal (C), and sagittal (D) images and fluid-attenuated inversion recovery (FLAIR) axial image (B) showing a 2.5 × 3.3 × 3.1 cm mass (white arrows) with extensive peritumoral vasogenic edema (yellow arrows). Of note, this patient had the highest expression of miR-20b in the resected tumor specimen.
Mentions: A total of 20 breast cancer cases met the study criteria. These included 11 breast cancer cases with brain metastasis and 9 age, stage and follow-up matched breast cancer cases without brain metastasis [12]. Figure 1 shows the typical radiographic features of a patient who underwent resection of a solitary breast cancer brain metastasis.

Bottom Line: However, unique molecular biomarkers have not yet been established.We further tested the effect of miR-20b overexpression on colony formation and invasion in vitro using MCF-7 and MDA-MB-231 cells.Further, miR-20b levels were observed to be high in brain-metastasizing cells, compared to bone-metastasizing cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Wayne State University School of Medicine and Karmanos Cancer Institute, Detroit, Michigan, USA.

ABSTRACT
Brain metastases are frequent in patients with advanced breast cancer and are associated with poor prognosis. However, unique molecular biomarkers have not yet been established. We hypothesized that microRNA-20b (miR-20b) plays a role in breast cancer brain metastasis. Our study cohort comprised of eleven breast cancer patients with brain metastasis and nine control patients (age, stage, and follow-up matched) with breast cancer without brain metastasis. Cases were reviewed microscopically to select tumor blocks with >50% tumor cells, RNA was extracted from formalin-fixed paraffin-embedded (FFPE) tumor tissue blocks and expression of miR-20b analyzed using qRT-PCR. We further tested the effect of miR-20b overexpression on colony formation and invasion in vitro using MCF-7 and MDA-MB-231 cells. In the patient-derived samples, miR-20b expression was significantly higher in brain metastases of breast cancer patients, compared to primary breast tumors as well as the patients without brain metastasis. miR-20b also significantly induced the colony formation and invasiveness of breast cancer cells. Further, miR-20b levels were observed to be high in brain-metastasizing cells, compared to bone-metastasizing cells. Together, our findings suggest a novel role of miR-20b in breast cancer brain metastasis that warrants further investigation for its potential to be developed as prognostic and/or therapeutic target.

No MeSH data available.


Related in: MedlinePlus