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Ubiquitin E3 ligase MARCH7 promotes ovarian tumor growth.

Hu J, Meng Y, Yu T, Hu L, Mao M - Oncotarget (2015)

Bottom Line: We found that expression of MARCH7 was higher in ovarian cancer tissues than normal ovarian tissues.Finally, MARCH7 was regulated by miR-101.Thus, MARCH7 is oncogenic and a potential target (oncotarget) for ovarian cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.

ABSTRACT
Ubiquitin E3 ligase MARCH7 is involved in T cell proliferation and neuronal development. We found that expression of MARCH7 was higher in ovarian cancer tissues than normal ovarian tissues. Silencing MARCH7 decreased cell proliferation, migration, and invasion. Ectopic expression of MARCH7 increased cell proliferation, migration and invasion. Silencing MARCH7 prevented ovarian cancer growth in mice. Silencing MARCH7 inhibited NFkB and Wnt/β-catenin pathway. In agreement, ectopically expressed MARCH7 activated NFkB and Wnt/β-catenin pathways. Finally, MARCH7 was regulated by miR-101. Thus, MARCH7 is oncogenic and a potential target (oncotarget) for ovarian cancer therapy.

No MeSH data available.


Related in: MedlinePlus

MARCH7 regulated the proliferation of ovarian cancer SKOV3 and A2780 cells(A) The relative expression of MARCH7 mRNA in ovarian cancer cell lines. (B, C) MARCH7 mRNA and protein level were down-regulated by infected with LV3-shMARCH7-1 or LV3-shMARCH7-2. (D, E) Ovarian cancer SKOV3 cells were infected with LV3-NC, LV3-shMARCH7-1 and LV3-shMARCH7-2. Cell proliferation was assessed by EdU. The proliferation rate of LV3-shMARCH7-1 and LV3-shMARCH7-2 cells was lower than that of LV3-NC cells. Original magnification, 200X. (F) Cell proliferation was determined by CCK-8 assay. (G, H) Colony formation assay. Skov3 cells infected with Lv3-shMarch7-1 or Lv3-shMarch7-2 were cultured by seeding 1,000 cells in 6-well plates and enumerating the number of colonies formed in 2 weeks. The number of colony formation of LV3-shMARCH7-1 or LV3-shMARCH7-2 was lower as compared with LV3-NC. (I, J) Ovarian cancer A2780 cells were infected with LV5-GFP or LV5-MARCH7. Then cell proliferation was determined by EdU. Original magnification, 200X. The proliferation rate of LV5-MARCH7 cells was increased compared with LV5-GFP cells. (K) Cell proliferation was determined by CCK-8 assay. (L, M) Colony formation assay. A2780 cells infected with LV5-MARCH7 were cultured by seeding 1000 cells in 6-well plates and counting the number of colonies formed in 2 weeks. The number of colony formation of LV5-MARCH7 was higher as compared with LV5-GFP. Error bars represent standard error. * p < 0.05, and **p < 0.001.
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Figure 2: MARCH7 regulated the proliferation of ovarian cancer SKOV3 and A2780 cells(A) The relative expression of MARCH7 mRNA in ovarian cancer cell lines. (B, C) MARCH7 mRNA and protein level were down-regulated by infected with LV3-shMARCH7-1 or LV3-shMARCH7-2. (D, E) Ovarian cancer SKOV3 cells were infected with LV3-NC, LV3-shMARCH7-1 and LV3-shMARCH7-2. Cell proliferation was assessed by EdU. The proliferation rate of LV3-shMARCH7-1 and LV3-shMARCH7-2 cells was lower than that of LV3-NC cells. Original magnification, 200X. (F) Cell proliferation was determined by CCK-8 assay. (G, H) Colony formation assay. Skov3 cells infected with Lv3-shMarch7-1 or Lv3-shMarch7-2 were cultured by seeding 1,000 cells in 6-well plates and enumerating the number of colonies formed in 2 weeks. The number of colony formation of LV3-shMARCH7-1 or LV3-shMARCH7-2 was lower as compared with LV3-NC. (I, J) Ovarian cancer A2780 cells were infected with LV5-GFP or LV5-MARCH7. Then cell proliferation was determined by EdU. Original magnification, 200X. The proliferation rate of LV5-MARCH7 cells was increased compared with LV5-GFP cells. (K) Cell proliferation was determined by CCK-8 assay. (L, M) Colony formation assay. A2780 cells infected with LV5-MARCH7 were cultured by seeding 1000 cells in 6-well plates and counting the number of colonies formed in 2 weeks. The number of colony formation of LV5-MARCH7 was higher as compared with LV5-GFP. Error bars represent standard error. * p < 0.05, and **p < 0.001.

Mentions: The expression of MARCH7 was investigated in 7 cell lines at the mRNA level by real-time quantitative PCR (qPCR) to select suitable cell lines for functional assays. Of these, MARCH7 expression was higher in the SKOV3, CaOV-3, and Es-2 cell lines, than in the A2780 cell line (Fig. 2A). Therefore, A2780 cell line was selected for exogenous expression; SKOV3 cell was selected for down -regulation of MARCH7 to determine the MARCH7 functions. The mRNA and protein level of MARCH7 was decreased in LV3-shMARCH7-1 or LV3-shMARCH7-2 infected SKOV3 cells compared with LV3-NC SKOV3 cells (Fig. 2B and 2C).


Ubiquitin E3 ligase MARCH7 promotes ovarian tumor growth.

Hu J, Meng Y, Yu T, Hu L, Mao M - Oncotarget (2015)

MARCH7 regulated the proliferation of ovarian cancer SKOV3 and A2780 cells(A) The relative expression of MARCH7 mRNA in ovarian cancer cell lines. (B, C) MARCH7 mRNA and protein level were down-regulated by infected with LV3-shMARCH7-1 or LV3-shMARCH7-2. (D, E) Ovarian cancer SKOV3 cells were infected with LV3-NC, LV3-shMARCH7-1 and LV3-shMARCH7-2. Cell proliferation was assessed by EdU. The proliferation rate of LV3-shMARCH7-1 and LV3-shMARCH7-2 cells was lower than that of LV3-NC cells. Original magnification, 200X. (F) Cell proliferation was determined by CCK-8 assay. (G, H) Colony formation assay. Skov3 cells infected with Lv3-shMarch7-1 or Lv3-shMarch7-2 were cultured by seeding 1,000 cells in 6-well plates and enumerating the number of colonies formed in 2 weeks. The number of colony formation of LV3-shMARCH7-1 or LV3-shMARCH7-2 was lower as compared with LV3-NC. (I, J) Ovarian cancer A2780 cells were infected with LV5-GFP or LV5-MARCH7. Then cell proliferation was determined by EdU. Original magnification, 200X. The proliferation rate of LV5-MARCH7 cells was increased compared with LV5-GFP cells. (K) Cell proliferation was determined by CCK-8 assay. (L, M) Colony formation assay. A2780 cells infected with LV5-MARCH7 were cultured by seeding 1000 cells in 6-well plates and counting the number of colonies formed in 2 weeks. The number of colony formation of LV5-MARCH7 was higher as compared with LV5-GFP. Error bars represent standard error. * p < 0.05, and **p < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Figure 2: MARCH7 regulated the proliferation of ovarian cancer SKOV3 and A2780 cells(A) The relative expression of MARCH7 mRNA in ovarian cancer cell lines. (B, C) MARCH7 mRNA and protein level were down-regulated by infected with LV3-shMARCH7-1 or LV3-shMARCH7-2. (D, E) Ovarian cancer SKOV3 cells were infected with LV3-NC, LV3-shMARCH7-1 and LV3-shMARCH7-2. Cell proliferation was assessed by EdU. The proliferation rate of LV3-shMARCH7-1 and LV3-shMARCH7-2 cells was lower than that of LV3-NC cells. Original magnification, 200X. (F) Cell proliferation was determined by CCK-8 assay. (G, H) Colony formation assay. Skov3 cells infected with Lv3-shMarch7-1 or Lv3-shMarch7-2 were cultured by seeding 1,000 cells in 6-well plates and enumerating the number of colonies formed in 2 weeks. The number of colony formation of LV3-shMARCH7-1 or LV3-shMARCH7-2 was lower as compared with LV3-NC. (I, J) Ovarian cancer A2780 cells were infected with LV5-GFP or LV5-MARCH7. Then cell proliferation was determined by EdU. Original magnification, 200X. The proliferation rate of LV5-MARCH7 cells was increased compared with LV5-GFP cells. (K) Cell proliferation was determined by CCK-8 assay. (L, M) Colony formation assay. A2780 cells infected with LV5-MARCH7 were cultured by seeding 1000 cells in 6-well plates and counting the number of colonies formed in 2 weeks. The number of colony formation of LV5-MARCH7 was higher as compared with LV5-GFP. Error bars represent standard error. * p < 0.05, and **p < 0.001.
Mentions: The expression of MARCH7 was investigated in 7 cell lines at the mRNA level by real-time quantitative PCR (qPCR) to select suitable cell lines for functional assays. Of these, MARCH7 expression was higher in the SKOV3, CaOV-3, and Es-2 cell lines, than in the A2780 cell line (Fig. 2A). Therefore, A2780 cell line was selected for exogenous expression; SKOV3 cell was selected for down -regulation of MARCH7 to determine the MARCH7 functions. The mRNA and protein level of MARCH7 was decreased in LV3-shMARCH7-1 or LV3-shMARCH7-2 infected SKOV3 cells compared with LV3-NC SKOV3 cells (Fig. 2B and 2C).

Bottom Line: We found that expression of MARCH7 was higher in ovarian cancer tissues than normal ovarian tissues.Finally, MARCH7 was regulated by miR-101.Thus, MARCH7 is oncogenic and a potential target (oncotarget) for ovarian cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.

ABSTRACT
Ubiquitin E3 ligase MARCH7 is involved in T cell proliferation and neuronal development. We found that expression of MARCH7 was higher in ovarian cancer tissues than normal ovarian tissues. Silencing MARCH7 decreased cell proliferation, migration, and invasion. Ectopic expression of MARCH7 increased cell proliferation, migration and invasion. Silencing MARCH7 prevented ovarian cancer growth in mice. Silencing MARCH7 inhibited NFkB and Wnt/β-catenin pathway. In agreement, ectopically expressed MARCH7 activated NFkB and Wnt/β-catenin pathways. Finally, MARCH7 was regulated by miR-101. Thus, MARCH7 is oncogenic and a potential target (oncotarget) for ovarian cancer therapy.

No MeSH data available.


Related in: MedlinePlus