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The impact of insulin on chemotherapeutic sensitivity to 5-fluorouracil in gastric cancer cell lines SGC7901, MKN45 and MKN28.

Wei Z, Liang L, Junsong L, Rui C, Shuai C, Guanglin Q, Shicai H, Zexing W, Jin W, Xiangming C, Shufeng W - J. Exp. Clin. Cancer Res. (2015)

Bottom Line: The expression of P-glycoprotein was determined by Western blotting.Addition of 1 μM insulin remarkably promoted the proliferation of SGC7901, MKN45 and MKN28 cells and decreased the cytotoxicity of 5-fluorouracil.In addition, the expression of P-glycoprotein was upregulated in SGC7901, MKN45 and MKN28 cells.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, P.R.China. zhaowei9803@126.com.

ABSTRACT

Background: The role of insulin in the pathogenesis of cancer has been increasingly emphasized because of the high incidence of obesity and metabolic syndrome and their correlated complication including cancer. This study aimed to explore the impact of insulin on chemoresistance to 5-fluorouracil in gastric cancer and the possible mechanisms.

Methods: Tissue samples of gastric cancer and adjacent normal gastric mucosa from patients with or without obesity were performed immunohistochemical staining for P-glycoprotein. The follow-up was done after the surgical treatment. The effect of insulin on chemotherapeutic sensitivity of the three gastric cancer cell lines to 5-fluorouracil was evaluated by pre-incubation with insulin before administration of 5-fluorouracil. The expression of P-glycoprotein was determined by Western blotting.

Results: P-glycoprotein were overexpressed in tissues from patients who suffered gastric cancer and were higher in those simultaneously suffered gastric cancer and obesity. Addition of 1 μM insulin remarkably promoted the proliferation of SGC7901, MKN45 and MKN28 cells and decreased the cytotoxicity of 5-fluorouracil. In addition, the expression of P-glycoprotein was upregulated in SGC7901, MKN45 and MKN28 cells.

Conclusion: Insulin improved the proliferation of gastric cancer cell lines and contributed to chemoresistance of gastric cancer cells to 5-fluorouracil which is likely to involve upregulation of P-glycoprotein.

No MeSH data available.


Related in: MedlinePlus

Immunoblotting of P-gp expression in three gastric cancer cell lines in comparison with GES cell line under the condition of adding different concentrations of insulin and 5-fluorouracil at 72 h. A is the blotting of P-gp and B is the densitometry.
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Fig5: Immunoblotting of P-gp expression in three gastric cancer cell lines in comparison with GES cell line under the condition of adding different concentrations of insulin and 5-fluorouracil at 72 h. A is the blotting of P-gp and B is the densitometry.

Mentions: We used Western-blot analyses to investigate the expression of P-glycoprotein in different gastric cancer cell lines with or without administration of insulin and 5-fluorouracil. As shown in Figure 5, P-glycoprotein expression increased in gastric cancer cells after treatment of 5-fluorouracil compared with GES cells. With the presence of insulin, the level of P-glycoprotein was highly upregulated in gastric cancer cells in contrast to the nonobvious alteration in GES cells. These densitometry analysis was in accordance with immunoblotting (Figure 5).Figure 5


The impact of insulin on chemotherapeutic sensitivity to 5-fluorouracil in gastric cancer cell lines SGC7901, MKN45 and MKN28.

Wei Z, Liang L, Junsong L, Rui C, Shuai C, Guanglin Q, Shicai H, Zexing W, Jin W, Xiangming C, Shufeng W - J. Exp. Clin. Cancer Res. (2015)

Immunoblotting of P-gp expression in three gastric cancer cell lines in comparison with GES cell line under the condition of adding different concentrations of insulin and 5-fluorouracil at 72 h. A is the blotting of P-gp and B is the densitometry.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4494778&req=5

Fig5: Immunoblotting of P-gp expression in three gastric cancer cell lines in comparison with GES cell line under the condition of adding different concentrations of insulin and 5-fluorouracil at 72 h. A is the blotting of P-gp and B is the densitometry.
Mentions: We used Western-blot analyses to investigate the expression of P-glycoprotein in different gastric cancer cell lines with or without administration of insulin and 5-fluorouracil. As shown in Figure 5, P-glycoprotein expression increased in gastric cancer cells after treatment of 5-fluorouracil compared with GES cells. With the presence of insulin, the level of P-glycoprotein was highly upregulated in gastric cancer cells in contrast to the nonobvious alteration in GES cells. These densitometry analysis was in accordance with immunoblotting (Figure 5).Figure 5

Bottom Line: The expression of P-glycoprotein was determined by Western blotting.Addition of 1 μM insulin remarkably promoted the proliferation of SGC7901, MKN45 and MKN28 cells and decreased the cytotoxicity of 5-fluorouracil.In addition, the expression of P-glycoprotein was upregulated in SGC7901, MKN45 and MKN28 cells.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, P.R.China. zhaowei9803@126.com.

ABSTRACT

Background: The role of insulin in the pathogenesis of cancer has been increasingly emphasized because of the high incidence of obesity and metabolic syndrome and their correlated complication including cancer. This study aimed to explore the impact of insulin on chemoresistance to 5-fluorouracil in gastric cancer and the possible mechanisms.

Methods: Tissue samples of gastric cancer and adjacent normal gastric mucosa from patients with or without obesity were performed immunohistochemical staining for P-glycoprotein. The follow-up was done after the surgical treatment. The effect of insulin on chemotherapeutic sensitivity of the three gastric cancer cell lines to 5-fluorouracil was evaluated by pre-incubation with insulin before administration of 5-fluorouracil. The expression of P-glycoprotein was determined by Western blotting.

Results: P-glycoprotein were overexpressed in tissues from patients who suffered gastric cancer and were higher in those simultaneously suffered gastric cancer and obesity. Addition of 1 μM insulin remarkably promoted the proliferation of SGC7901, MKN45 and MKN28 cells and decreased the cytotoxicity of 5-fluorouracil. In addition, the expression of P-glycoprotein was upregulated in SGC7901, MKN45 and MKN28 cells.

Conclusion: Insulin improved the proliferation of gastric cancer cell lines and contributed to chemoresistance of gastric cancer cells to 5-fluorouracil which is likely to involve upregulation of P-glycoprotein.

No MeSH data available.


Related in: MedlinePlus