Evaluation of coronary adventitial vasa vasorum using 3D optical coherence tomography--animal and human studies.
Bottom Line: In OCT images, adventitial MC was identified as signal-voiding areas which were located within 1 mm from the lumen-intima border.In the human study, there was a significant positive correlation between MCV and PV (segment number = 24, r(2) = 0.63, P < 0.01).Our results suggest that evaluation of MCV with 3D OCT imaging might be a novel method to estimate the amount of adventitial VV in vivo, and further has the potential to provide a pathophysiological insight into a role of the VV in allograft vasculopathy.
Affiliation: Division of Cardiovascular Diseases, Mayo Clinic, Rochester, MN, USA.Show MeSH
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Mentions: The 3-D reconstruction and analysis was performed with the ANALYZE software 11.0 (Biomedical Imaging Resource, Rochester, Minnesota), which was demonstrated as the useful modality of 3D volumetric analysis of IVUS images 14. DICOM file of OCT images was loaded as red channel data with 8 bit matrix of 20*20*200 μm cubic voxels. MC areas and lumen-intima borders are traced in every cross-sectional OCT image slices separated by a distance of 200 μm. Adventitial MC was defined as signal-voiding tubular or layer structures with major diameters from 50 to 300 micrometer 15, which were observed in at least 2 consecutive slices, and located within 1 mm from the lumen-intima border (Figure 1A and 1B). After volume rendering process, which provides a variety of display representation of 3-D image data sets, 3D pattern of MC was determined visually (Figure 1C). Then, volumetric analysis of MC and lumen was performed in every 1 mm segment consisted of 5 OCT image slices.
Affiliation: Division of Cardiovascular Diseases, Mayo Clinic, Rochester, MN, USA.