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Evaluation of coronary adventitial vasa vasorum using 3D optical coherence tomography--animal and human studies.

Aoki T, Rodriguez-Porcel M, Matsuo Y, Cassar A, Kwon TG, Franchi F, Gulati R, Kushwaha SS, Lennon RJ, Lerman LO, Ritman EL, Lerman A - Atherosclerosis (2015)

Bottom Line: In OCT images, adventitial MC was identified as signal-voiding areas which were located within 1 mm from the lumen-intima border.In the human study, there was a significant positive correlation between MCV and PV (segment number = 24, r(2) = 0.63, P < 0.01).Our results suggest that evaluation of MCV with 3D OCT imaging might be a novel method to estimate the amount of adventitial VV in vivo, and further has the potential to provide a pathophysiological insight into a role of the VV in allograft vasculopathy.

View Article: PubMed Central - PubMed

Affiliation: Division of Cardiovascular Diseases, Mayo Clinic, Rochester, MN, USA.

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OCT and m-CT Images of Swine Carotid ArteryCross sectional images of OCT (A) with trace line (B) at the level of dashed line in 3D OCT image (C). The white arrows indicate corresponding microvessels in both of OCT and m-CT (Figure 2). The arrow heads means mismatched vessels between both images. Areas surrounded by yellow and green lines indicate MC; lumen area, by red line. MC are depicted in green; MC used for segment matching, yellow; Vessel lumen, red. White bar indicates 1 mm.Cross sectional images of m-CT (D) with trace line (E) at the level of dashed line in 3D m-CT image (F). The white arrows indicate corresponding VV to MC in OCT images. Areas surrounded by green lines indicate VV; lumen area, red. MC is depicted in green; vessel lumen, red. White bar indicates 1 mm.MC, microchannels; m-CT, micro-computerized tomography; OCT, optical coherence tomography; VV, vasa vasorum.
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Figure 1: OCT and m-CT Images of Swine Carotid ArteryCross sectional images of OCT (A) with trace line (B) at the level of dashed line in 3D OCT image (C). The white arrows indicate corresponding microvessels in both of OCT and m-CT (Figure 2). The arrow heads means mismatched vessels between both images. Areas surrounded by yellow and green lines indicate MC; lumen area, by red line. MC are depicted in green; MC used for segment matching, yellow; Vessel lumen, red. White bar indicates 1 mm.Cross sectional images of m-CT (D) with trace line (E) at the level of dashed line in 3D m-CT image (F). The white arrows indicate corresponding VV to MC in OCT images. Areas surrounded by green lines indicate VV; lumen area, red. MC is depicted in green; vessel lumen, red. White bar indicates 1 mm.MC, microchannels; m-CT, micro-computerized tomography; OCT, optical coherence tomography; VV, vasa vasorum.

Mentions: The 3-D reconstruction and analysis was performed with the ANALYZE software 11.0 (Biomedical Imaging Resource, Rochester, Minnesota), which was demonstrated as the useful modality of 3D volumetric analysis of IVUS images 14. DICOM file of OCT images was loaded as red channel data with 8 bit matrix of 20*20*200 μm cubic voxels. MC areas and lumen-intima borders are traced in every cross-sectional OCT image slices separated by a distance of 200 μm. Adventitial MC was defined as signal-voiding tubular or layer structures with major diameters from 50 to 300 micrometer 15, which were observed in at least 2 consecutive slices, and located within 1 mm from the lumen-intima border (Figure 1A and 1B). After volume rendering process, which provides a variety of display representation of 3-D image data sets, 3D pattern of MC was determined visually (Figure 1C). Then, volumetric analysis of MC and lumen was performed in every 1 mm segment consisted of 5 OCT image slices.


Evaluation of coronary adventitial vasa vasorum using 3D optical coherence tomography--animal and human studies.

Aoki T, Rodriguez-Porcel M, Matsuo Y, Cassar A, Kwon TG, Franchi F, Gulati R, Kushwaha SS, Lennon RJ, Lerman LO, Ritman EL, Lerman A - Atherosclerosis (2015)

OCT and m-CT Images of Swine Carotid ArteryCross sectional images of OCT (A) with trace line (B) at the level of dashed line in 3D OCT image (C). The white arrows indicate corresponding microvessels in both of OCT and m-CT (Figure 2). The arrow heads means mismatched vessels between both images. Areas surrounded by yellow and green lines indicate MC; lumen area, by red line. MC are depicted in green; MC used for segment matching, yellow; Vessel lumen, red. White bar indicates 1 mm.Cross sectional images of m-CT (D) with trace line (E) at the level of dashed line in 3D m-CT image (F). The white arrows indicate corresponding VV to MC in OCT images. Areas surrounded by green lines indicate VV; lumen area, red. MC is depicted in green; vessel lumen, red. White bar indicates 1 mm.MC, microchannels; m-CT, micro-computerized tomography; OCT, optical coherence tomography; VV, vasa vasorum.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494669&req=5

Figure 1: OCT and m-CT Images of Swine Carotid ArteryCross sectional images of OCT (A) with trace line (B) at the level of dashed line in 3D OCT image (C). The white arrows indicate corresponding microvessels in both of OCT and m-CT (Figure 2). The arrow heads means mismatched vessels between both images. Areas surrounded by yellow and green lines indicate MC; lumen area, by red line. MC are depicted in green; MC used for segment matching, yellow; Vessel lumen, red. White bar indicates 1 mm.Cross sectional images of m-CT (D) with trace line (E) at the level of dashed line in 3D m-CT image (F). The white arrows indicate corresponding VV to MC in OCT images. Areas surrounded by green lines indicate VV; lumen area, red. MC is depicted in green; vessel lumen, red. White bar indicates 1 mm.MC, microchannels; m-CT, micro-computerized tomography; OCT, optical coherence tomography; VV, vasa vasorum.
Mentions: The 3-D reconstruction and analysis was performed with the ANALYZE software 11.0 (Biomedical Imaging Resource, Rochester, Minnesota), which was demonstrated as the useful modality of 3D volumetric analysis of IVUS images 14. DICOM file of OCT images was loaded as red channel data with 8 bit matrix of 20*20*200 μm cubic voxels. MC areas and lumen-intima borders are traced in every cross-sectional OCT image slices separated by a distance of 200 μm. Adventitial MC was defined as signal-voiding tubular or layer structures with major diameters from 50 to 300 micrometer 15, which were observed in at least 2 consecutive slices, and located within 1 mm from the lumen-intima border (Figure 1A and 1B). After volume rendering process, which provides a variety of display representation of 3-D image data sets, 3D pattern of MC was determined visually (Figure 1C). Then, volumetric analysis of MC and lumen was performed in every 1 mm segment consisted of 5 OCT image slices.

Bottom Line: In OCT images, adventitial MC was identified as signal-voiding areas which were located within 1 mm from the lumen-intima border.In the human study, there was a significant positive correlation between MCV and PV (segment number = 24, r(2) = 0.63, P < 0.01).Our results suggest that evaluation of MCV with 3D OCT imaging might be a novel method to estimate the amount of adventitial VV in vivo, and further has the potential to provide a pathophysiological insight into a role of the VV in allograft vasculopathy.

View Article: PubMed Central - PubMed

Affiliation: Division of Cardiovascular Diseases, Mayo Clinic, Rochester, MN, USA.

Show MeSH
Related in: MedlinePlus