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The reduction of volume and fiber bundle connections in the hippocampus of EGR3 transgenic schizophrenia rats.

Ma E, Song T, Zhang H, Lu J, Wang L, Zhao Q, Guo R, Li M, Ma G, Lu G, Li K - Neuropsychiatr Dis Treat (2015)

Bottom Line: MedINRIA software was used for data processing of diffusion tensor and fiber bundles tracking.The fibronectin in relevant brain regions was also analyzed.A significant reduction in the volume and number of the fiber bundles was also observed in left prefrontal-left hippocampus, left hippocampus-left thalamus, left prefrontal-left hippocampus-left thalamus areas in the model group (all P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Imaging, Jinling Hospital, Medical School of Nanjing University, Nanjing, People's Republic of China ; Department of Radiology, China-Japan Friendship Hospital, Beijing, People's Republic of China.

ABSTRACT

Background and objective: There is a growing consensus that schizophrenia is ultimately caused by abnormal communication between spatially disparate brain structures. White matter fasciculi represent the primary infrastructure for long distance communication in the brain. In this study, we aimed to investigate the white matter connection in schizophrenia susceptible brain regions of early growth response factor 3 (EGR3) expressing rats.

Methods: A rat model of schizophrenia was created by the transfection of the EGR3 gene into rat hippocampus. All animals were placed in a fixation system using a commercial rat-dedicated coil. Schizophrenia susceptible brain regions were scanned using in vivo diffusion tensor magnetic resonance imaging. The volume, quantity, average length of fiber bundles, fractional anisotropy, apparent diffusion coefficient, the relative heterosexual fraction, and volume ratio were collected in the whole brain and schizophrenia related brain areas (the hippocampus, thalamus, and prefrontal lobe). MedINRIA software was used for data processing of diffusion tensor and fiber bundles tracking. The fibronectin in relevant brain regions was also analyzed.

Results: There was a significant decrease in the volume of the fiber beam through the left hippocampus dentate in the schizophrenia model group in comparison to the control group and the risperidone treatment group (P<0.05). A significant reduction in the volume and number of the fiber bundles was also observed in left prefrontal-left hippocampus, left hippocampus-left thalamus, left prefrontal-left hippocampus-left thalamus areas in the model group (all P<0.05).

Conclusion: The volume of hippocampus and the number of fiber bundles were reduced in EGR3 transgenic schizophrenia rats, and are the most sensitive indicators in schizophrenia. The diffusion tensor imaging technique plays an important role in the evaluation of patients with schizophrenia.

No MeSH data available.


Related in: MedlinePlus

White fiber connection between left hippocampus and left thalamus.Notes: a, control; b, sham group; c, ERG3 transfection; d, risperidone treatment group. (A) DTI demonstrates the white fiber bundle connection on coronal view; (B) statistical analysis of fiber volume, P=0.0022 for control vs schizophrenia in volume and P=0.0136 for control vs model group in number.Abbreviations:EGR3, early growth response factor 3; DTI, diffusion tensor imaging.
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f5-ndt-11-1625: White fiber connection between left hippocampus and left thalamus.Notes: a, control; b, sham group; c, ERG3 transfection; d, risperidone treatment group. (A) DTI demonstrates the white fiber bundle connection on coronal view; (B) statistical analysis of fiber volume, P=0.0022 for control vs schizophrenia in volume and P=0.0136 for control vs model group in number.Abbreviations:EGR3, early growth response factor 3; DTI, diffusion tensor imaging.

Mentions: The volume and number of fiber connections between the left anterior frontal lobe and the left hippocampus were significantly decreased in the EGR3 transfection schizophrenic group compared with the other three groups (Figure 4). There were no significant differences in the length, FA, ADC, RA, and VR of the fiber connections (data not shown). Similarly, we found a significant reduction of fiber connections (both volume and number) between the left hippocampus and the left thalamus and the connections were restored after risperidone treatment (Figure 5). In addition, both the number and volume of the three consecutive connections among left anterior frontal lobe–left hippocampus–left thalamus were significantly decreased in the EGR3 gene transfection schizophrenic group (Figure 6). No changes in the fiber length were noticed. The volume and number of connections were restored after risperidone treatment.


The reduction of volume and fiber bundle connections in the hippocampus of EGR3 transgenic schizophrenia rats.

Ma E, Song T, Zhang H, Lu J, Wang L, Zhao Q, Guo R, Li M, Ma G, Lu G, Li K - Neuropsychiatr Dis Treat (2015)

White fiber connection between left hippocampus and left thalamus.Notes: a, control; b, sham group; c, ERG3 transfection; d, risperidone treatment group. (A) DTI demonstrates the white fiber bundle connection on coronal view; (B) statistical analysis of fiber volume, P=0.0022 for control vs schizophrenia in volume and P=0.0136 for control vs model group in number.Abbreviations:EGR3, early growth response factor 3; DTI, diffusion tensor imaging.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494618&req=5

f5-ndt-11-1625: White fiber connection between left hippocampus and left thalamus.Notes: a, control; b, sham group; c, ERG3 transfection; d, risperidone treatment group. (A) DTI demonstrates the white fiber bundle connection on coronal view; (B) statistical analysis of fiber volume, P=0.0022 for control vs schizophrenia in volume and P=0.0136 for control vs model group in number.Abbreviations:EGR3, early growth response factor 3; DTI, diffusion tensor imaging.
Mentions: The volume and number of fiber connections between the left anterior frontal lobe and the left hippocampus were significantly decreased in the EGR3 transfection schizophrenic group compared with the other three groups (Figure 4). There were no significant differences in the length, FA, ADC, RA, and VR of the fiber connections (data not shown). Similarly, we found a significant reduction of fiber connections (both volume and number) between the left hippocampus and the left thalamus and the connections were restored after risperidone treatment (Figure 5). In addition, both the number and volume of the three consecutive connections among left anterior frontal lobe–left hippocampus–left thalamus were significantly decreased in the EGR3 gene transfection schizophrenic group (Figure 6). No changes in the fiber length were noticed. The volume and number of connections were restored after risperidone treatment.

Bottom Line: MedINRIA software was used for data processing of diffusion tensor and fiber bundles tracking.The fibronectin in relevant brain regions was also analyzed.A significant reduction in the volume and number of the fiber bundles was also observed in left prefrontal-left hippocampus, left hippocampus-left thalamus, left prefrontal-left hippocampus-left thalamus areas in the model group (all P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Imaging, Jinling Hospital, Medical School of Nanjing University, Nanjing, People's Republic of China ; Department of Radiology, China-Japan Friendship Hospital, Beijing, People's Republic of China.

ABSTRACT

Background and objective: There is a growing consensus that schizophrenia is ultimately caused by abnormal communication between spatially disparate brain structures. White matter fasciculi represent the primary infrastructure for long distance communication in the brain. In this study, we aimed to investigate the white matter connection in schizophrenia susceptible brain regions of early growth response factor 3 (EGR3) expressing rats.

Methods: A rat model of schizophrenia was created by the transfection of the EGR3 gene into rat hippocampus. All animals were placed in a fixation system using a commercial rat-dedicated coil. Schizophrenia susceptible brain regions were scanned using in vivo diffusion tensor magnetic resonance imaging. The volume, quantity, average length of fiber bundles, fractional anisotropy, apparent diffusion coefficient, the relative heterosexual fraction, and volume ratio were collected in the whole brain and schizophrenia related brain areas (the hippocampus, thalamus, and prefrontal lobe). MedINRIA software was used for data processing of diffusion tensor and fiber bundles tracking. The fibronectin in relevant brain regions was also analyzed.

Results: There was a significant decrease in the volume of the fiber beam through the left hippocampus dentate in the schizophrenia model group in comparison to the control group and the risperidone treatment group (P<0.05). A significant reduction in the volume and number of the fiber bundles was also observed in left prefrontal-left hippocampus, left hippocampus-left thalamus, left prefrontal-left hippocampus-left thalamus areas in the model group (all P<0.05).

Conclusion: The volume of hippocampus and the number of fiber bundles were reduced in EGR3 transgenic schizophrenia rats, and are the most sensitive indicators in schizophrenia. The diffusion tensor imaging technique plays an important role in the evaluation of patients with schizophrenia.

No MeSH data available.


Related in: MedlinePlus