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The reduction of volume and fiber bundle connections in the hippocampus of EGR3 transgenic schizophrenia rats.

Ma E, Song T, Zhang H, Lu J, Wang L, Zhao Q, Guo R, Li M, Ma G, Lu G, Li K - Neuropsychiatr Dis Treat (2015)

Bottom Line: MedINRIA software was used for data processing of diffusion tensor and fiber bundles tracking.The fibronectin in relevant brain regions was also analyzed.A significant reduction in the volume and number of the fiber bundles was also observed in left prefrontal-left hippocampus, left hippocampus-left thalamus, left prefrontal-left hippocampus-left thalamus areas in the model group (all P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Imaging, Jinling Hospital, Medical School of Nanjing University, Nanjing, People's Republic of China ; Department of Radiology, China-Japan Friendship Hospital, Beijing, People's Republic of China.

ABSTRACT

Background and objective: There is a growing consensus that schizophrenia is ultimately caused by abnormal communication between spatially disparate brain structures. White matter fasciculi represent the primary infrastructure for long distance communication in the brain. In this study, we aimed to investigate the white matter connection in schizophrenia susceptible brain regions of early growth response factor 3 (EGR3) expressing rats.

Methods: A rat model of schizophrenia was created by the transfection of the EGR3 gene into rat hippocampus. All animals were placed in a fixation system using a commercial rat-dedicated coil. Schizophrenia susceptible brain regions were scanned using in vivo diffusion tensor magnetic resonance imaging. The volume, quantity, average length of fiber bundles, fractional anisotropy, apparent diffusion coefficient, the relative heterosexual fraction, and volume ratio were collected in the whole brain and schizophrenia related brain areas (the hippocampus, thalamus, and prefrontal lobe). MedINRIA software was used for data processing of diffusion tensor and fiber bundles tracking. The fibronectin in relevant brain regions was also analyzed.

Results: There was a significant decrease in the volume of the fiber beam through the left hippocampus dentate in the schizophrenia model group in comparison to the control group and the risperidone treatment group (P<0.05). A significant reduction in the volume and number of the fiber bundles was also observed in left prefrontal-left hippocampus, left hippocampus-left thalamus, left prefrontal-left hippocampus-left thalamus areas in the model group (all P<0.05).

Conclusion: The volume of hippocampus and the number of fiber bundles were reduced in EGR3 transgenic schizophrenia rats, and are the most sensitive indicators in schizophrenia. The diffusion tensor imaging technique plays an important role in the evaluation of patients with schizophrenia.

No MeSH data available.


Related in: MedlinePlus

Significant reduction of white fiber bundle volume in left hippocampus of rats with EGR3 gene transfection.Notes: a, control; b, sham group; c, ERG3 transfection model group; d, risperidone treatment group. (A) Registration of white fiber bundle and anatomy; (B) DTI demonstrates the white fiber bundle on coronal view; (C) DTI demonstrates the white fiber bundle on axial view; (D) statistical analysis of white fiber volume, P=0.039 for control vs model group and P=0.046 for schizophrenia vs risperidone treatment group. The color in B and C indicates the relative directions (0°–90°) of fiber bundles in brain white matter. Blue color (0°) indicates the fiber bundles were horizontal to the X-axis. Pink color indicates the fiber bundles were vertical to the X-axis.Abbreviations:EGR3, early growth response factor 3; DTI, diffusion tensor imaging.
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f1-ndt-11-1625: Significant reduction of white fiber bundle volume in left hippocampus of rats with EGR3 gene transfection.Notes: a, control; b, sham group; c, ERG3 transfection model group; d, risperidone treatment group. (A) Registration of white fiber bundle and anatomy; (B) DTI demonstrates the white fiber bundle on coronal view; (C) DTI demonstrates the white fiber bundle on axial view; (D) statistical analysis of white fiber volume, P=0.039 for control vs model group and P=0.046 for schizophrenia vs risperidone treatment group. The color in B and C indicates the relative directions (0°–90°) of fiber bundles in brain white matter. Blue color (0°) indicates the fiber bundles were horizontal to the X-axis. Pink color indicates the fiber bundles were vertical to the X-axis.Abbreviations:EGR3, early growth response factor 3; DTI, diffusion tensor imaging.

Mentions: The white fiber bundle volume in left hippocampus were remarkably decreased in the EGR3 gene transfection schizophrenic group compared with that in the control group and the sham group (Figure 1). Risperidone treatment restored the volume of white fiber back to normal (Figure 1). No significant changes were observed in the length, FA, ADC, RA, and VR in the left hippocampus among the four groups (P>0.05) (Table 3).


The reduction of volume and fiber bundle connections in the hippocampus of EGR3 transgenic schizophrenia rats.

Ma E, Song T, Zhang H, Lu J, Wang L, Zhao Q, Guo R, Li M, Ma G, Lu G, Li K - Neuropsychiatr Dis Treat (2015)

Significant reduction of white fiber bundle volume in left hippocampus of rats with EGR3 gene transfection.Notes: a, control; b, sham group; c, ERG3 transfection model group; d, risperidone treatment group. (A) Registration of white fiber bundle and anatomy; (B) DTI demonstrates the white fiber bundle on coronal view; (C) DTI demonstrates the white fiber bundle on axial view; (D) statistical analysis of white fiber volume, P=0.039 for control vs model group and P=0.046 for schizophrenia vs risperidone treatment group. The color in B and C indicates the relative directions (0°–90°) of fiber bundles in brain white matter. Blue color (0°) indicates the fiber bundles were horizontal to the X-axis. Pink color indicates the fiber bundles were vertical to the X-axis.Abbreviations:EGR3, early growth response factor 3; DTI, diffusion tensor imaging.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494618&req=5

f1-ndt-11-1625: Significant reduction of white fiber bundle volume in left hippocampus of rats with EGR3 gene transfection.Notes: a, control; b, sham group; c, ERG3 transfection model group; d, risperidone treatment group. (A) Registration of white fiber bundle and anatomy; (B) DTI demonstrates the white fiber bundle on coronal view; (C) DTI demonstrates the white fiber bundle on axial view; (D) statistical analysis of white fiber volume, P=0.039 for control vs model group and P=0.046 for schizophrenia vs risperidone treatment group. The color in B and C indicates the relative directions (0°–90°) of fiber bundles in brain white matter. Blue color (0°) indicates the fiber bundles were horizontal to the X-axis. Pink color indicates the fiber bundles were vertical to the X-axis.Abbreviations:EGR3, early growth response factor 3; DTI, diffusion tensor imaging.
Mentions: The white fiber bundle volume in left hippocampus were remarkably decreased in the EGR3 gene transfection schizophrenic group compared with that in the control group and the sham group (Figure 1). Risperidone treatment restored the volume of white fiber back to normal (Figure 1). No significant changes were observed in the length, FA, ADC, RA, and VR in the left hippocampus among the four groups (P>0.05) (Table 3).

Bottom Line: MedINRIA software was used for data processing of diffusion tensor and fiber bundles tracking.The fibronectin in relevant brain regions was also analyzed.A significant reduction in the volume and number of the fiber bundles was also observed in left prefrontal-left hippocampus, left hippocampus-left thalamus, left prefrontal-left hippocampus-left thalamus areas in the model group (all P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Imaging, Jinling Hospital, Medical School of Nanjing University, Nanjing, People's Republic of China ; Department of Radiology, China-Japan Friendship Hospital, Beijing, People's Republic of China.

ABSTRACT

Background and objective: There is a growing consensus that schizophrenia is ultimately caused by abnormal communication between spatially disparate brain structures. White matter fasciculi represent the primary infrastructure for long distance communication in the brain. In this study, we aimed to investigate the white matter connection in schizophrenia susceptible brain regions of early growth response factor 3 (EGR3) expressing rats.

Methods: A rat model of schizophrenia was created by the transfection of the EGR3 gene into rat hippocampus. All animals were placed in a fixation system using a commercial rat-dedicated coil. Schizophrenia susceptible brain regions were scanned using in vivo diffusion tensor magnetic resonance imaging. The volume, quantity, average length of fiber bundles, fractional anisotropy, apparent diffusion coefficient, the relative heterosexual fraction, and volume ratio were collected in the whole brain and schizophrenia related brain areas (the hippocampus, thalamus, and prefrontal lobe). MedINRIA software was used for data processing of diffusion tensor and fiber bundles tracking. The fibronectin in relevant brain regions was also analyzed.

Results: There was a significant decrease in the volume of the fiber beam through the left hippocampus dentate in the schizophrenia model group in comparison to the control group and the risperidone treatment group (P<0.05). A significant reduction in the volume and number of the fiber bundles was also observed in left prefrontal-left hippocampus, left hippocampus-left thalamus, left prefrontal-left hippocampus-left thalamus areas in the model group (all P<0.05).

Conclusion: The volume of hippocampus and the number of fiber bundles were reduced in EGR3 transgenic schizophrenia rats, and are the most sensitive indicators in schizophrenia. The diffusion tensor imaging technique plays an important role in the evaluation of patients with schizophrenia.

No MeSH data available.


Related in: MedlinePlus