Limits...
Cellular response of chondrocytes to magnesium alloys for orthopedic applications.

Liao Y, Xu Q, Zhang J, Niu J, Yuan G, Jiang Y, He Y, Wang X - Int. J. Mol. Med. (2015)

Bottom Line: There were no statistically significant differences observed between the JDBM, AZ31, WE43 and pure Mg group (p>0.05).These findings suggested that the JDBM alloy was highly biocompatible with chondrocytes in vitro, yielding results similar to those of AZ31, WE43 and pure Mg.Furthermore, CaHPO4·2H2O coating significantly improved the biocompatibility of this alloy.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.

ABSTRACT
In the present study, the effects of Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO4·2H2O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg) and Ti alloy (TC4) on rabbit chondrocytes were investigated in vitro. Adhesion experiments revealed the satisfactory morphology of chondrocytes on the surface of all samples. An indirect cytotoxicity test using MTT assay revealed that C‑JDBM and TC4 exhibited results similar to those of the negative control, better than those obtained with JDBM, AZ31, WE43 and pure Mg (p<0.05). There were no statistically significant differences observed between the JDBM, AZ31, WE43 and pure Mg group (p>0.05). The results of indirect cell cytotoxicity and proliferation assays, as well as those of apoptosis assay, glycosaminoglycan (GAG) quantification, assessment of collagen Ⅱ (Col Ⅱ) levels and RT-qPCR revealed a similar a trend as was observed with MTT assay. These findings suggested that the JDBM alloy was highly biocompatible with chondrocytes in vitro, yielding results similar to those of AZ31, WE43 and pure Mg. Furthermore, CaHPO4·2H2O coating significantly improved the biocompatibility of this alloy.

No MeSH data available.


Related in: MedlinePlus

Aggrecan and collagen II (Col II) gene expression relative to GAPDH after 5 days of incubation with the Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO4·2H2O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg), and TC4 extraction media, with Dulbecco’s modified Eagle’s medium with F12 (DMEM/F12) as the control. *P<0.05 (DMEM/F12, C-JDBM and TC4 vs. JDBM, AZ31, WE43, and Mg). #P>0.05 (DMEM/F12 vs. C-JDBM vs. TC4, JDBM vs. AZ31 vs. WE43 vs. Mg). Negative control, cells cultured in DMEM/F12 alone; positive control, cells cultured in DMEM/F12 medium with 10% DMSO.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4494570&req=5

f7-ijmm-36-01-0073: Aggrecan and collagen II (Col II) gene expression relative to GAPDH after 5 days of incubation with the Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO4·2H2O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg), and TC4 extraction media, with Dulbecco’s modified Eagle’s medium with F12 (DMEM/F12) as the control. *P<0.05 (DMEM/F12, C-JDBM and TC4 vs. JDBM, AZ31, WE43, and Mg). #P>0.05 (DMEM/F12 vs. C-JDBM vs. TC4, JDBM vs. AZ31 vs. WE43 vs. Mg). Negative control, cells cultured in DMEM/F12 alone; positive control, cells cultured in DMEM/F12 medium with 10% DMSO.

Mentions: As shown in Fig. 7, the results of the analysis of the relative mRNA expression levels of aggrecan and Col II in the chondrocytes following 5 days of incubation under the different treatment conditions were consistent with those obtained form the other assays (Figs. 3–6) and with the results of our previous study (12). The results revealed that the cytocompatibility of JDBM was comparable to that of AZ31, WE43 and pure Mg. Furthermore, the CaHPO4·2H2O coating significantly improved its compatibility.


Cellular response of chondrocytes to magnesium alloys for orthopedic applications.

Liao Y, Xu Q, Zhang J, Niu J, Yuan G, Jiang Y, He Y, Wang X - Int. J. Mol. Med. (2015)

Aggrecan and collagen II (Col II) gene expression relative to GAPDH after 5 days of incubation with the Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO4·2H2O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg), and TC4 extraction media, with Dulbecco’s modified Eagle’s medium with F12 (DMEM/F12) as the control. *P<0.05 (DMEM/F12, C-JDBM and TC4 vs. JDBM, AZ31, WE43, and Mg). #P>0.05 (DMEM/F12 vs. C-JDBM vs. TC4, JDBM vs. AZ31 vs. WE43 vs. Mg). Negative control, cells cultured in DMEM/F12 alone; positive control, cells cultured in DMEM/F12 medium with 10% DMSO.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494570&req=5

f7-ijmm-36-01-0073: Aggrecan and collagen II (Col II) gene expression relative to GAPDH after 5 days of incubation with the Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO4·2H2O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg), and TC4 extraction media, with Dulbecco’s modified Eagle’s medium with F12 (DMEM/F12) as the control. *P<0.05 (DMEM/F12, C-JDBM and TC4 vs. JDBM, AZ31, WE43, and Mg). #P>0.05 (DMEM/F12 vs. C-JDBM vs. TC4, JDBM vs. AZ31 vs. WE43 vs. Mg). Negative control, cells cultured in DMEM/F12 alone; positive control, cells cultured in DMEM/F12 medium with 10% DMSO.
Mentions: As shown in Fig. 7, the results of the analysis of the relative mRNA expression levels of aggrecan and Col II in the chondrocytes following 5 days of incubation under the different treatment conditions were consistent with those obtained form the other assays (Figs. 3–6) and with the results of our previous study (12). The results revealed that the cytocompatibility of JDBM was comparable to that of AZ31, WE43 and pure Mg. Furthermore, the CaHPO4·2H2O coating significantly improved its compatibility.

Bottom Line: There were no statistically significant differences observed between the JDBM, AZ31, WE43 and pure Mg group (p>0.05).These findings suggested that the JDBM alloy was highly biocompatible with chondrocytes in vitro, yielding results similar to those of AZ31, WE43 and pure Mg.Furthermore, CaHPO4·2H2O coating significantly improved the biocompatibility of this alloy.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.

ABSTRACT
In the present study, the effects of Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO4·2H2O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg) and Ti alloy (TC4) on rabbit chondrocytes were investigated in vitro. Adhesion experiments revealed the satisfactory morphology of chondrocytes on the surface of all samples. An indirect cytotoxicity test using MTT assay revealed that C‑JDBM and TC4 exhibited results similar to those of the negative control, better than those obtained with JDBM, AZ31, WE43 and pure Mg (p<0.05). There were no statistically significant differences observed between the JDBM, AZ31, WE43 and pure Mg group (p>0.05). The results of indirect cell cytotoxicity and proliferation assays, as well as those of apoptosis assay, glycosaminoglycan (GAG) quantification, assessment of collagen Ⅱ (Col Ⅱ) levels and RT-qPCR revealed a similar a trend as was observed with MTT assay. These findings suggested that the JDBM alloy was highly biocompatible with chondrocytes in vitro, yielding results similar to those of AZ31, WE43 and pure Mg. Furthermore, CaHPO4·2H2O coating significantly improved the biocompatibility of this alloy.

No MeSH data available.


Related in: MedlinePlus