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Cellular response of chondrocytes to magnesium alloys for orthopedic applications.

Liao Y, Xu Q, Zhang J, Niu J, Yuan G, Jiang Y, He Y, Wang X - Int. J. Mol. Med. (2015)

Bottom Line: There were no statistically significant differences observed between the JDBM, AZ31, WE43 and pure Mg group (p>0.05).These findings suggested that the JDBM alloy was highly biocompatible with chondrocytes in vitro, yielding results similar to those of AZ31, WE43 and pure Mg.Furthermore, CaHPO4·2H2O coating significantly improved the biocompatibility of this alloy.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.

ABSTRACT
In the present study, the effects of Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO4·2H2O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg) and Ti alloy (TC4) on rabbit chondrocytes were investigated in vitro. Adhesion experiments revealed the satisfactory morphology of chondrocytes on the surface of all samples. An indirect cytotoxicity test using MTT assay revealed that C‑JDBM and TC4 exhibited results similar to those of the negative control, better than those obtained with JDBM, AZ31, WE43 and pure Mg (p<0.05). There were no statistically significant differences observed between the JDBM, AZ31, WE43 and pure Mg group (p>0.05). The results of indirect cell cytotoxicity and proliferation assays, as well as those of apoptosis assay, glycosaminoglycan (GAG) quantification, assessment of collagen Ⅱ (Col Ⅱ) levels and RT-qPCR revealed a similar a trend as was observed with MTT assay. These findings suggested that the JDBM alloy was highly biocompatible with chondrocytes in vitro, yielding results similar to those of AZ31, WE43 and pure Mg. Furthermore, CaHPO4·2H2O coating significantly improved the biocompatibility of this alloy.

No MeSH data available.


Related in: MedlinePlus

4′,6-Diamidino-2-phenylindole (DAPI) staining of chondrocytes (x400 magnification). Cells cultured for 3 days on (A) brushite (CaHPO4·2H2O)-co ated JDBM (C-JDBM), (B) Mg-Nd-Zn-Zr (JDBM), (C) AZ31, (D) WE43, (E) pure magnesium (Mg), and (F) TC4. Cells cultured for 1 day on (G) C-JDBM, (H) pure Mg, and (I) TC4. A few cells were already observed on the samples after 1 day of culture (G–I). After 3 days of culture, the number of cells on the discs increased (A–F). Among the cultures, a significantly greater number of adhered cells was observed on the surfaces of the C-JDBM and TC4 discs (A and F, respectively), whereas the least number of cells was observed on the WE43 disc (D).
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f2-ijmm-36-01-0073: 4′,6-Diamidino-2-phenylindole (DAPI) staining of chondrocytes (x400 magnification). Cells cultured for 3 days on (A) brushite (CaHPO4·2H2O)-co ated JDBM (C-JDBM), (B) Mg-Nd-Zn-Zr (JDBM), (C) AZ31, (D) WE43, (E) pure magnesium (Mg), and (F) TC4. Cells cultured for 1 day on (G) C-JDBM, (H) pure Mg, and (I) TC4. A few cells were already observed on the samples after 1 day of culture (G–I). After 3 days of culture, the number of cells on the discs increased (A–F). Among the cultures, a significantly greater number of adhered cells was observed on the surfaces of the C-JDBM and TC4 discs (A and F, respectively), whereas the least number of cells was observed on the WE43 disc (D).

Mentions: The morphologies of the chondrocytes cultured on the discs of JDBM, C-JDBM, AZ31, WE43, pure Mg and TC4 for 1 or 3 days are illustrated in Figs. 1 and 2.


Cellular response of chondrocytes to magnesium alloys for orthopedic applications.

Liao Y, Xu Q, Zhang J, Niu J, Yuan G, Jiang Y, He Y, Wang X - Int. J. Mol. Med. (2015)

4′,6-Diamidino-2-phenylindole (DAPI) staining of chondrocytes (x400 magnification). Cells cultured for 3 days on (A) brushite (CaHPO4·2H2O)-co ated JDBM (C-JDBM), (B) Mg-Nd-Zn-Zr (JDBM), (C) AZ31, (D) WE43, (E) pure magnesium (Mg), and (F) TC4. Cells cultured for 1 day on (G) C-JDBM, (H) pure Mg, and (I) TC4. A few cells were already observed on the samples after 1 day of culture (G–I). After 3 days of culture, the number of cells on the discs increased (A–F). Among the cultures, a significantly greater number of adhered cells was observed on the surfaces of the C-JDBM and TC4 discs (A and F, respectively), whereas the least number of cells was observed on the WE43 disc (D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494570&req=5

f2-ijmm-36-01-0073: 4′,6-Diamidino-2-phenylindole (DAPI) staining of chondrocytes (x400 magnification). Cells cultured for 3 days on (A) brushite (CaHPO4·2H2O)-co ated JDBM (C-JDBM), (B) Mg-Nd-Zn-Zr (JDBM), (C) AZ31, (D) WE43, (E) pure magnesium (Mg), and (F) TC4. Cells cultured for 1 day on (G) C-JDBM, (H) pure Mg, and (I) TC4. A few cells were already observed on the samples after 1 day of culture (G–I). After 3 days of culture, the number of cells on the discs increased (A–F). Among the cultures, a significantly greater number of adhered cells was observed on the surfaces of the C-JDBM and TC4 discs (A and F, respectively), whereas the least number of cells was observed on the WE43 disc (D).
Mentions: The morphologies of the chondrocytes cultured on the discs of JDBM, C-JDBM, AZ31, WE43, pure Mg and TC4 for 1 or 3 days are illustrated in Figs. 1 and 2.

Bottom Line: There were no statistically significant differences observed between the JDBM, AZ31, WE43 and pure Mg group (p>0.05).These findings suggested that the JDBM alloy was highly biocompatible with chondrocytes in vitro, yielding results similar to those of AZ31, WE43 and pure Mg.Furthermore, CaHPO4·2H2O coating significantly improved the biocompatibility of this alloy.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China.

ABSTRACT
In the present study, the effects of Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO4·2H2O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg) and Ti alloy (TC4) on rabbit chondrocytes were investigated in vitro. Adhesion experiments revealed the satisfactory morphology of chondrocytes on the surface of all samples. An indirect cytotoxicity test using MTT assay revealed that C‑JDBM and TC4 exhibited results similar to those of the negative control, better than those obtained with JDBM, AZ31, WE43 and pure Mg (p<0.05). There were no statistically significant differences observed between the JDBM, AZ31, WE43 and pure Mg group (p>0.05). The results of indirect cell cytotoxicity and proliferation assays, as well as those of apoptosis assay, glycosaminoglycan (GAG) quantification, assessment of collagen Ⅱ (Col Ⅱ) levels and RT-qPCR revealed a similar a trend as was observed with MTT assay. These findings suggested that the JDBM alloy was highly biocompatible with chondrocytes in vitro, yielding results similar to those of AZ31, WE43 and pure Mg. Furthermore, CaHPO4·2H2O coating significantly improved the biocompatibility of this alloy.

No MeSH data available.


Related in: MedlinePlus