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A Novel Rat Model of Intramedullary Tibia Fracture Fixation Using Polyetheretherketone Threaded Rod.

Jin Z, Sun Y, Guan Y, Yu G - Plast Reconstr Surg Glob Open (2015)

Bottom Line: X-ray examination was performed at 0, 4, and 8 weeks postoperatively.Histological analysis was conducted via hematoxylin-eosin staining of nondecalcified tissue sections.This innovative model is simple and effective, providing a new selection in future biomedical research.

View Article: PubMed Central - PubMed

Affiliation: Department of Joint Surgery and Sports Medicine, First Hospital of China Medical University, Shenyang, China; and Department of Radiological Diagnosis and Interventional Treatment, First Hospital of China Medical University, Shenyang, China.

ABSTRACT

Background: The rat fracture fixation models have been widely adopted, but current implant designs suffer from operational difficulty, massive soft-tissue dissection, and radiological intervention. The authors developed a new tibia fracture-healing model using minor invasive intramedullary fixations with polyetheretherketone (PEEK) threaded rods, which have excellent x-ray translucency and no magnetic resonance artifact.

Methods: Tibia fractures of 6 adult male Sprague-Dawley rats were fixed with intramedullary PEEK threaded rods. X-ray examination was performed at 0, 4, and 8 weeks postoperatively. Histological analysis was conducted via hematoxylin-eosin staining of nondecalcified tissue sections.

Results: Radiological fracture healing was observed at 8 weeks postoperatively. Histology demonstrated fracture gap bridging and bone ingrowth adjacent to PEEK.

Conclusion: This innovative model is simple and effective, providing a new selection in future biomedical research.

No MeSH data available.


Related in: MedlinePlus

A, Surgical procedure: bone tunnel reaming with PEEK threaded rod. B, Surgical procedure: fracture fixation with anatomical alignment and compression.
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Figure 1: A, Surgical procedure: bone tunnel reaming with PEEK threaded rod. B, Surgical procedure: fracture fixation with anatomical alignment and compression.

Mentions: Before operation, animals received intraperitoneal injections of 50 mg/kg pentobarbital sodium (Merck, Darmstadt, Germany) for general anesthesia. The left hind leg was shaved and disinfected. A 3-cm longitudinal anteromedial skin incision was made to expose the left knee joint and tibia. The patella was released laterally, and a 2-mm diameter bone tunnel was drilled from proximal tibial plateau with a depth of 20 mm, followed by another reaming step with PEEK threaded rod (Fig. 1). The level of osteotomy was at the proximal third of tibia. After transverse osteotomy, a 20-mm PEEK threaded rod was inserted by a press-fit maneuver, and care was taken to ensure rigid fixation without extra bone damage (Fig. 1). Then, the wound was irrigated with normal saline, and the muscle fascia and skin were sutured interruptedly. Intraoperative lateral radiographs were obtained after wound closure using standard imaging module of an x-ray unit (Arcadis; Siemens, Muenchen, Germany). Postoperative plain films were obtained at 4 and 8 weeks to monitor healing status.


A Novel Rat Model of Intramedullary Tibia Fracture Fixation Using Polyetheretherketone Threaded Rod.

Jin Z, Sun Y, Guan Y, Yu G - Plast Reconstr Surg Glob Open (2015)

A, Surgical procedure: bone tunnel reaming with PEEK threaded rod. B, Surgical procedure: fracture fixation with anatomical alignment and compression.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4494487&req=5

Figure 1: A, Surgical procedure: bone tunnel reaming with PEEK threaded rod. B, Surgical procedure: fracture fixation with anatomical alignment and compression.
Mentions: Before operation, animals received intraperitoneal injections of 50 mg/kg pentobarbital sodium (Merck, Darmstadt, Germany) for general anesthesia. The left hind leg was shaved and disinfected. A 3-cm longitudinal anteromedial skin incision was made to expose the left knee joint and tibia. The patella was released laterally, and a 2-mm diameter bone tunnel was drilled from proximal tibial plateau with a depth of 20 mm, followed by another reaming step with PEEK threaded rod (Fig. 1). The level of osteotomy was at the proximal third of tibia. After transverse osteotomy, a 20-mm PEEK threaded rod was inserted by a press-fit maneuver, and care was taken to ensure rigid fixation without extra bone damage (Fig. 1). Then, the wound was irrigated with normal saline, and the muscle fascia and skin were sutured interruptedly. Intraoperative lateral radiographs were obtained after wound closure using standard imaging module of an x-ray unit (Arcadis; Siemens, Muenchen, Germany). Postoperative plain films were obtained at 4 and 8 weeks to monitor healing status.

Bottom Line: X-ray examination was performed at 0, 4, and 8 weeks postoperatively.Histological analysis was conducted via hematoxylin-eosin staining of nondecalcified tissue sections.This innovative model is simple and effective, providing a new selection in future biomedical research.

View Article: PubMed Central - PubMed

Affiliation: Department of Joint Surgery and Sports Medicine, First Hospital of China Medical University, Shenyang, China; and Department of Radiological Diagnosis and Interventional Treatment, First Hospital of China Medical University, Shenyang, China.

ABSTRACT

Background: The rat fracture fixation models have been widely adopted, but current implant designs suffer from operational difficulty, massive soft-tissue dissection, and radiological intervention. The authors developed a new tibia fracture-healing model using minor invasive intramedullary fixations with polyetheretherketone (PEEK) threaded rods, which have excellent x-ray translucency and no magnetic resonance artifact.

Methods: Tibia fractures of 6 adult male Sprague-Dawley rats were fixed with intramedullary PEEK threaded rods. X-ray examination was performed at 0, 4, and 8 weeks postoperatively. Histological analysis was conducted via hematoxylin-eosin staining of nondecalcified tissue sections.

Results: Radiological fracture healing was observed at 8 weeks postoperatively. Histology demonstrated fracture gap bridging and bone ingrowth adjacent to PEEK.

Conclusion: This innovative model is simple and effective, providing a new selection in future biomedical research.

No MeSH data available.


Related in: MedlinePlus