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Effectively Axonal-supercharged Interpositional Jump-Graft with an Artificial Nerve Conduit for Rat Facial Nerve Paralysis.

Niimi Y, Matsumine H, Takeuchi Y, Sasaki R, Watanabe Y, Yamato M, Miyata M, Sakurai H - Plast Reconstr Surg Glob Open (2015)

Bottom Line: Interpositional jump graft (IPJG) is a nerve graft axonally supercharged from the hypoglossal nerve.Thirteen weeks after the surgery, the outcome was histologically and physiologically compared with conventional IPJG with autograft using the great auricular nerve.In the autograft and silicone tube groups, the regeneration of myelinated axons was observed.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Reconstructive Surgery, Tokyo Women's Medical University, Yachiyo Medical Center, Yachiyo-shi, Chiba, Japan; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan; Department of Plastic and Reconstructive Surgery, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan; Department of Physiology, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan; and Department of Oral and Maxillofacial Surgery, Global Center of Excellence (COE) Program, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan.

ABSTRACT

Background: Interpositional jump graft (IPJG) is a nerve graft axonally supercharged from the hypoglossal nerve. However, for using the technique, an autologous nerve, which should contain the great auricular and sural nerves, must be obtained. Depending on the donor site, unavoidable issues such as nerve disorders and postoperative scarring may appear. To reduce the issues, in this study, the authors developed an end-to-side neurorrhaphy technique with the recipient nerve and an artificial nerve conduit and investigated the efficacy of an IPJG with an artificial nerve conduit in a rat facial nerve paresis model.

Methods: A ligature clip was used to crush the facial nerve trunk, thereby creating a partial facial nerve paresis model. An artificial nerve conduit was then prepared with a 10-mm-long silicone tube containing 10 μL type I collagen and used to create an IPJG between the facial nerve trunk and the hypoglossal nerve (the silicone tube group). Thirteen weeks after the surgery, the outcome was histologically and physiologically compared with conventional IPJG with autograft using the great auricular nerve.

Results: Retrograde tracer test confirmed a double innervation by the facial and hypoglossal nerve nuclei. In the autograft and silicone tube groups, the regeneration of myelinated axons was observed.

Conclusion: In this study, the authors successfully developed an end-to-side neurorrhaphy technique with the recipient nerve and an artificial nerve conduit, and revealed that an IPJG in the conduit was effective in the rat facial nerve paresis model.

No MeSH data available.


Related in: MedlinePlus

Macroscopical observations of IPJGs with autograft and in a silicone tube conduit, and the toluidine blue staining of their sections at postoperative week 13. A, IPJG with autograft. B, IPJG in a silicone tube conduit. C, Toluidine blue section of IPJG with autograft. D, Toluidine blue section of regenerated IPJG.
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Figure 3: Macroscopical observations of IPJGs with autograft and in a silicone tube conduit, and the toluidine blue staining of their sections at postoperative week 13. A, IPJG with autograft. B, IPJG in a silicone tube conduit. C, Toluidine blue section of IPJG with autograft. D, Toluidine blue section of regenerated IPJG.

Mentions: On macro view, at postoperative week 7, no myelin sheath regeneration was observed in the silicone tube group. At postoperative week 13, the neuronal regeneration rates were 71% (n = 5/7) in the silicone tube group (Fig. 3B) and 100% (n = 7/7) in the autograft group (Fig. 3A). On toluidine blue–stained histological sections, the surrounding area was covered with a thick epineurium, and the regeneration of myelin sheath was observed in the center in the silicone tube group (Fig. 3D). Although, in the autograft group, regenerated nerve was found but covered with granulation tissue, several thick axonal blood vessels appeared to be running alongside the epineurium, and the regeneration of a dense myelin sheath was observed (Fig. 3C).


Effectively Axonal-supercharged Interpositional Jump-Graft with an Artificial Nerve Conduit for Rat Facial Nerve Paralysis.

Niimi Y, Matsumine H, Takeuchi Y, Sasaki R, Watanabe Y, Yamato M, Miyata M, Sakurai H - Plast Reconstr Surg Glob Open (2015)

Macroscopical observations of IPJGs with autograft and in a silicone tube conduit, and the toluidine blue staining of their sections at postoperative week 13. A, IPJG with autograft. B, IPJG in a silicone tube conduit. C, Toluidine blue section of IPJG with autograft. D, Toluidine blue section of regenerated IPJG.
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Related In: Results  -  Collection

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Figure 3: Macroscopical observations of IPJGs with autograft and in a silicone tube conduit, and the toluidine blue staining of their sections at postoperative week 13. A, IPJG with autograft. B, IPJG in a silicone tube conduit. C, Toluidine blue section of IPJG with autograft. D, Toluidine blue section of regenerated IPJG.
Mentions: On macro view, at postoperative week 7, no myelin sheath regeneration was observed in the silicone tube group. At postoperative week 13, the neuronal regeneration rates were 71% (n = 5/7) in the silicone tube group (Fig. 3B) and 100% (n = 7/7) in the autograft group (Fig. 3A). On toluidine blue–stained histological sections, the surrounding area was covered with a thick epineurium, and the regeneration of myelin sheath was observed in the center in the silicone tube group (Fig. 3D). Although, in the autograft group, regenerated nerve was found but covered with granulation tissue, several thick axonal blood vessels appeared to be running alongside the epineurium, and the regeneration of a dense myelin sheath was observed (Fig. 3C).

Bottom Line: Interpositional jump graft (IPJG) is a nerve graft axonally supercharged from the hypoglossal nerve.Thirteen weeks after the surgery, the outcome was histologically and physiologically compared with conventional IPJG with autograft using the great auricular nerve.In the autograft and silicone tube groups, the regeneration of myelinated axons was observed.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Reconstructive Surgery, Tokyo Women's Medical University, Yachiyo Medical Center, Yachiyo-shi, Chiba, Japan; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan; Department of Plastic and Reconstructive Surgery, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan; Department of Physiology, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan; and Department of Oral and Maxillofacial Surgery, Global Center of Excellence (COE) Program, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan.

ABSTRACT

Background: Interpositional jump graft (IPJG) is a nerve graft axonally supercharged from the hypoglossal nerve. However, for using the technique, an autologous nerve, which should contain the great auricular and sural nerves, must be obtained. Depending on the donor site, unavoidable issues such as nerve disorders and postoperative scarring may appear. To reduce the issues, in this study, the authors developed an end-to-side neurorrhaphy technique with the recipient nerve and an artificial nerve conduit and investigated the efficacy of an IPJG with an artificial nerve conduit in a rat facial nerve paresis model.

Methods: A ligature clip was used to crush the facial nerve trunk, thereby creating a partial facial nerve paresis model. An artificial nerve conduit was then prepared with a 10-mm-long silicone tube containing 10 μL type I collagen and used to create an IPJG between the facial nerve trunk and the hypoglossal nerve (the silicone tube group). Thirteen weeks after the surgery, the outcome was histologically and physiologically compared with conventional IPJG with autograft using the great auricular nerve.

Results: Retrograde tracer test confirmed a double innervation by the facial and hypoglossal nerve nuclei. In the autograft and silicone tube groups, the regeneration of myelinated axons was observed.

Conclusion: In this study, the authors successfully developed an end-to-side neurorrhaphy technique with the recipient nerve and an artificial nerve conduit, and revealed that an IPJG in the conduit was effective in the rat facial nerve paresis model.

No MeSH data available.


Related in: MedlinePlus