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Effects of Transfer from Breeding to Research Facility on the Welfare of Rats.

Arts JW, Oosterhuis NR, Kramer K, Ohl F - Animals (Basel) (2014)

Bottom Line: Transfer from the breeding facility to a research facility is a stressful event for laboratory animals.This study measured ambient and body temperature, corticosterone and glucose levels, body weight, behavior and water and food intake before, during and after transfer in Wistar rats.Male rats need to habituate for at least one week, females for two weeks after transfer.

View Article: PubMed Central - PubMed

Affiliation: Department of Animals in Science & Society, Division of Animal Welfare & Laboratory Animal Science, Veterinary Faculty, Utrecht University, Utrecht, 3584 CM, The Netherlands. jarts@harlan.com.

ABSTRACT
Transfer from the breeding facility to a research facility is a stressful event for laboratory animals. Heat stress has been reported to constitute one of the major concerns during transport of animals. This study measured ambient and body temperature, corticosterone and glucose levels, body weight, behavior and water and food intake before, during and after transfer in Wistar rats. Decreased body weight, water and food intake were observed on the day of transfer in rats. Environmental temperature strongly affected body temperature of rats and needs to be controlled. Male rats need to habituate for at least one week, females for two weeks after transfer.

No MeSH data available.


Related in: MedlinePlus

Core body temperature Tb of transferred (triangles) and control (diamonds) rats and ambient temperature Ta inside transportation boxes (solid line) (°C ± SD) during transfer and in control area (dotted line) (top x-axis: time since packing, bottom x-axis: actual time). The black-white bar at bottom indicates light (white) and dark (black) periods. Different stages during transfer are included. n = 16, sexes pooled.
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animals-04-00712-f003: Core body temperature Tb of transferred (triangles) and control (diamonds) rats and ambient temperature Ta inside transportation boxes (solid line) (°C ± SD) during transfer and in control area (dotted line) (top x-axis: time since packing, bottom x-axis: actual time). The black-white bar at bottom indicates light (white) and dark (black) periods. Different stages during transfer are included. n = 16, sexes pooled.

Mentions: The highest Ta’s inside the transportation boxes were measured when transportation boxes were collected at the holding area of Harlan NL (male: 28.50 °C, female: 27.40 °C, Figure 3). Environmental temperature varied between 22 °C and 25 °C at the holding area. During transfer to CLARF Utrecht with the climate controlled van (15 °C), Ta inside transportation boxes with male rats decreased to 17.30 °C and 17.10 °C for female rats. During the period of transfer Ta at the housing facility of control groups varied between 24.1 °C and 25.1 °C.


Effects of Transfer from Breeding to Research Facility on the Welfare of Rats.

Arts JW, Oosterhuis NR, Kramer K, Ohl F - Animals (Basel) (2014)

Core body temperature Tb of transferred (triangles) and control (diamonds) rats and ambient temperature Ta inside transportation boxes (solid line) (°C ± SD) during transfer and in control area (dotted line) (top x-axis: time since packing, bottom x-axis: actual time). The black-white bar at bottom indicates light (white) and dark (black) periods. Different stages during transfer are included. n = 16, sexes pooled.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494421&req=5

animals-04-00712-f003: Core body temperature Tb of transferred (triangles) and control (diamonds) rats and ambient temperature Ta inside transportation boxes (solid line) (°C ± SD) during transfer and in control area (dotted line) (top x-axis: time since packing, bottom x-axis: actual time). The black-white bar at bottom indicates light (white) and dark (black) periods. Different stages during transfer are included. n = 16, sexes pooled.
Mentions: The highest Ta’s inside the transportation boxes were measured when transportation boxes were collected at the holding area of Harlan NL (male: 28.50 °C, female: 27.40 °C, Figure 3). Environmental temperature varied between 22 °C and 25 °C at the holding area. During transfer to CLARF Utrecht with the climate controlled van (15 °C), Ta inside transportation boxes with male rats decreased to 17.30 °C and 17.10 °C for female rats. During the period of transfer Ta at the housing facility of control groups varied between 24.1 °C and 25.1 °C.

Bottom Line: Transfer from the breeding facility to a research facility is a stressful event for laboratory animals.This study measured ambient and body temperature, corticosterone and glucose levels, body weight, behavior and water and food intake before, during and after transfer in Wistar rats.Male rats need to habituate for at least one week, females for two weeks after transfer.

View Article: PubMed Central - PubMed

Affiliation: Department of Animals in Science & Society, Division of Animal Welfare & Laboratory Animal Science, Veterinary Faculty, Utrecht University, Utrecht, 3584 CM, The Netherlands. jarts@harlan.com.

ABSTRACT
Transfer from the breeding facility to a research facility is a stressful event for laboratory animals. Heat stress has been reported to constitute one of the major concerns during transport of animals. This study measured ambient and body temperature, corticosterone and glucose levels, body weight, behavior and water and food intake before, during and after transfer in Wistar rats. Decreased body weight, water and food intake were observed on the day of transfer in rats. Environmental temperature strongly affected body temperature of rats and needs to be controlled. Male rats need to habituate for at least one week, females for two weeks after transfer.

No MeSH data available.


Related in: MedlinePlus