Limits...
Effects of Transfer from Breeding to Research Facility on the Welfare of Rats.

Arts JW, Oosterhuis NR, Kramer K, Ohl F - Animals (Basel) (2014)

Bottom Line: Transfer from the breeding facility to a research facility is a stressful event for laboratory animals.This study measured ambient and body temperature, corticosterone and glucose levels, body weight, behavior and water and food intake before, during and after transfer in Wistar rats.Male rats need to habituate for at least one week, females for two weeks after transfer.

View Article: PubMed Central - PubMed

Affiliation: Department of Animals in Science & Society, Division of Animal Welfare & Laboratory Animal Science, Veterinary Faculty, Utrecht University, Utrecht, 3584 CM, The Netherlands. jarts@harlan.com.

ABSTRACT
Transfer from the breeding facility to a research facility is a stressful event for laboratory animals. Heat stress has been reported to constitute one of the major concerns during transport of animals. This study measured ambient and body temperature, corticosterone and glucose levels, body weight, behavior and water and food intake before, during and after transfer in Wistar rats. Decreased body weight, water and food intake were observed on the day of transfer in rats. Environmental temperature strongly affected body temperature of rats and needs to be controlled. Male rats need to habituate for at least one week, females for two weeks after transfer.

No MeSH data available.


Related in: MedlinePlus

Core body temperature Tb (°C ± SD) in transferred (TF: triangles, solid line) and control (CO: diamonds, dotted line) rats during dark (black symbols) and light (white symbols) period, 7 days before (DBT) until 22 days after (DAT) transfer (n = 16), sexes pooled. * indicates significant difference between TF and CO.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4494421&req=5

animals-04-00712-f002: Core body temperature Tb (°C ± SD) in transferred (TF: triangles, solid line) and control (CO: diamonds, dotted line) rats during dark (black symbols) and light (white symbols) period, 7 days before (DBT) until 22 days after (DAT) transfer (n = 16), sexes pooled. * indicates significant difference between TF and CO.

Mentions: No sex effect was found for body temperature Tb, therefore data was pooled for analysis and the results are shown in Figure 2. During dark periods, Tb was increased for all groups compared to light periods, shown as a light period effect (F(1,52) = 160.50, p < 0.001), except on the day before transfer (DBT1) for transferred animals (Figure 2), when transferred rats were packed in transportation boxes and were transferred to the logistic center awaiting final transfer to the CLARF. During the dark period there was a before-after × treatment interaction (F(1,26) = 9.82, p = 0.004), which was not found during the light period. During the dark period of transfer a decreased Tb was found in both sexes (t(13) = −4.77, p = 0.001).


Effects of Transfer from Breeding to Research Facility on the Welfare of Rats.

Arts JW, Oosterhuis NR, Kramer K, Ohl F - Animals (Basel) (2014)

Core body temperature Tb (°C ± SD) in transferred (TF: triangles, solid line) and control (CO: diamonds, dotted line) rats during dark (black symbols) and light (white symbols) period, 7 days before (DBT) until 22 days after (DAT) transfer (n = 16), sexes pooled. * indicates significant difference between TF and CO.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494421&req=5

animals-04-00712-f002: Core body temperature Tb (°C ± SD) in transferred (TF: triangles, solid line) and control (CO: diamonds, dotted line) rats during dark (black symbols) and light (white symbols) period, 7 days before (DBT) until 22 days after (DAT) transfer (n = 16), sexes pooled. * indicates significant difference between TF and CO.
Mentions: No sex effect was found for body temperature Tb, therefore data was pooled for analysis and the results are shown in Figure 2. During dark periods, Tb was increased for all groups compared to light periods, shown as a light period effect (F(1,52) = 160.50, p < 0.001), except on the day before transfer (DBT1) for transferred animals (Figure 2), when transferred rats were packed in transportation boxes and were transferred to the logistic center awaiting final transfer to the CLARF. During the dark period there was a before-after × treatment interaction (F(1,26) = 9.82, p = 0.004), which was not found during the light period. During the dark period of transfer a decreased Tb was found in both sexes (t(13) = −4.77, p = 0.001).

Bottom Line: Transfer from the breeding facility to a research facility is a stressful event for laboratory animals.This study measured ambient and body temperature, corticosterone and glucose levels, body weight, behavior and water and food intake before, during and after transfer in Wistar rats.Male rats need to habituate for at least one week, females for two weeks after transfer.

View Article: PubMed Central - PubMed

Affiliation: Department of Animals in Science & Society, Division of Animal Welfare & Laboratory Animal Science, Veterinary Faculty, Utrecht University, Utrecht, 3584 CM, The Netherlands. jarts@harlan.com.

ABSTRACT
Transfer from the breeding facility to a research facility is a stressful event for laboratory animals. Heat stress has been reported to constitute one of the major concerns during transport of animals. This study measured ambient and body temperature, corticosterone and glucose levels, body weight, behavior and water and food intake before, during and after transfer in Wistar rats. Decreased body weight, water and food intake were observed on the day of transfer in rats. Environmental temperature strongly affected body temperature of rats and needs to be controlled. Male rats need to habituate for at least one week, females for two weeks after transfer.

No MeSH data available.


Related in: MedlinePlus