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Extending the Collection Duration of Breath Samples for Enteric Methane Emission Estimation Using the SF₆ Tracer Technique.

Pinares-Patiño C, Gere J, Williams K, Gratton R, Juliarena P, Molano G, MacLean S, Sandoval E, Taylor G, Koolaard J - Animals (Basel) (2012)

Bottom Line: Half of the lines collected samples into PVC yokes using a modified capillary system as commonly used in New Zealand (NZL), and half collected samples into stainless steel cylinders using a ball-bearing flow restrictor as used in Argentina (ARG), all within a 10-day time frame, either daily, across two consecutive 5-day periods or across one 10-day period (in duplicate).The NZL system had greater sampling success (97.3 vs. 79.5%) and yielded more consistent CH₄ emission estimates than the ARG system.Emission estimates from NZL daily, NZL 5-day and NZL 10-day samplings were 114, 110 and 111 g d(-1), respectively.

View Article: PubMed Central - PubMed

Affiliation: Grasslands Research Centre, AgResearch Limited, Palmerston North 4442, New Zealand. cesar.pinares@agresearch.co.nz.

ABSTRACT
The daily sample collection protocol of the sulphur hexafluoride (SF₆) tracer technique for the estimation of methane (CH₄) emissions from ruminants may not be practical under extensive grazing systems. Here, under controlled conditions, we evaluated extended periods of sampling as an alternative to daily sample collections. Eight rumen-fistulated cows were housed and fed lucerne silage to achieve common daily feed intakes of 6.4 kg dry matter per cow. Following SF₆ permeation tube dosing, eight sampling lines were fitted to the breath collection harness, so that a common gas mix was available to each line. Half of the lines collected samples into PVC yokes using a modified capillary system as commonly used in New Zealand (NZL), and half collected samples into stainless steel cylinders using a ball-bearing flow restrictor as used in Argentina (ARG), all within a 10-day time frame, either daily, across two consecutive 5-day periods or across one 10-day period (in duplicate). The NZL system had greater sampling success (97.3 vs. 79.5%) and yielded more consistent CH₄ emission estimates than the ARG system. Emission estimates from NZL daily, NZL 5-day and NZL 10-day samplings were 114, 110 and 111 g d(-1), respectively. Extended sample collection protocol may be feasible, but definitive evaluation of this alternative as well as sample collection systems is required under grazing situations before a decision on recommendation can be made.

No MeSH data available.


Each cow harness was fitted with eight breath sample lines to allow a similar source of ‘breath’ sample just above the animal’s nostrils. Half of the sample lines were allocated to the Argentinean modified system (ARG) and another half to the New Zealand modified system (NZL). Sampling lines allocated to the NZL system were enclosed within polyethylene tubing having a common sample inlet (inverted ‘Y’) and placed at the mid-point between two ARG sample lines. Each of the ball-bearing flow restrictors for the ARG system (the sample inlet) was covered by water-proof polyester material. All the samples lines were extended to the back sides of the animal crate.
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animals-02-00275-f001: Each cow harness was fitted with eight breath sample lines to allow a similar source of ‘breath’ sample just above the animal’s nostrils. Half of the sample lines were allocated to the Argentinean modified system (ARG) and another half to the New Zealand modified system (NZL). Sampling lines allocated to the NZL system were enclosed within polyethylene tubing having a common sample inlet (inverted ‘Y’) and placed at the mid-point between two ARG sample lines. Each of the ball-bearing flow restrictors for the ARG system (the sample inlet) was covered by water-proof polyester material. All the samples lines were extended to the back sides of the animal crate.

Mentions: Eight sample lines were fitted to the cow harness. All had a similar source of ‘breath’ sample just above the nostrils of each participating animal. Half of the sample lines were allocated to the NZL system and another half to the ARG system (Figure 1). All the sample lines were extended (5 m length) to the back sides of the crates out of reach of the animal, where the collection canisters were hung (Figure 2). The extension lines for the ARG system were 1/4" o.d. polyethylene tubing, whereas those for the NZL system were 1/8" o.d. nylon tubing. The ARG system used stainless steel cylinders (0.5 L volume) as sample collection devices with sample flow restricted by a steel ball-bearing [7]. Flow restriction was achieved by pressing a stainless steel ball (8 mm diameter) housed in a brass female thread socket (Part IR8GH, Casucci Automatizacion S.A., Buenos Aires, Argentina) by means of a 1/8" brass bolt (Part A-TX8G-NPT, Casucci Automatizacion S.A., Buenos Aires, Argentina). Each flow restrictor for the ARG system was housed into a 5 cm polyethylene tubing (12 mm i.d.), the end of which was covered by a polyester fabric (camping tent material) aimed at protecting the flow restrictor from water blockage (Figure 3).


Extending the Collection Duration of Breath Samples for Enteric Methane Emission Estimation Using the SF₆ Tracer Technique.

Pinares-Patiño C, Gere J, Williams K, Gratton R, Juliarena P, Molano G, MacLean S, Sandoval E, Taylor G, Koolaard J - Animals (Basel) (2012)

Each cow harness was fitted with eight breath sample lines to allow a similar source of ‘breath’ sample just above the animal’s nostrils. Half of the sample lines were allocated to the Argentinean modified system (ARG) and another half to the New Zealand modified system (NZL). Sampling lines allocated to the NZL system were enclosed within polyethylene tubing having a common sample inlet (inverted ‘Y’) and placed at the mid-point between two ARG sample lines. Each of the ball-bearing flow restrictors for the ARG system (the sample inlet) was covered by water-proof polyester material. All the samples lines were extended to the back sides of the animal crate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494328&req=5

animals-02-00275-f001: Each cow harness was fitted with eight breath sample lines to allow a similar source of ‘breath’ sample just above the animal’s nostrils. Half of the sample lines were allocated to the Argentinean modified system (ARG) and another half to the New Zealand modified system (NZL). Sampling lines allocated to the NZL system were enclosed within polyethylene tubing having a common sample inlet (inverted ‘Y’) and placed at the mid-point between two ARG sample lines. Each of the ball-bearing flow restrictors for the ARG system (the sample inlet) was covered by water-proof polyester material. All the samples lines were extended to the back sides of the animal crate.
Mentions: Eight sample lines were fitted to the cow harness. All had a similar source of ‘breath’ sample just above the nostrils of each participating animal. Half of the sample lines were allocated to the NZL system and another half to the ARG system (Figure 1). All the sample lines were extended (5 m length) to the back sides of the crates out of reach of the animal, where the collection canisters were hung (Figure 2). The extension lines for the ARG system were 1/4" o.d. polyethylene tubing, whereas those for the NZL system were 1/8" o.d. nylon tubing. The ARG system used stainless steel cylinders (0.5 L volume) as sample collection devices with sample flow restricted by a steel ball-bearing [7]. Flow restriction was achieved by pressing a stainless steel ball (8 mm diameter) housed in a brass female thread socket (Part IR8GH, Casucci Automatizacion S.A., Buenos Aires, Argentina) by means of a 1/8" brass bolt (Part A-TX8G-NPT, Casucci Automatizacion S.A., Buenos Aires, Argentina). Each flow restrictor for the ARG system was housed into a 5 cm polyethylene tubing (12 mm i.d.), the end of which was covered by a polyester fabric (camping tent material) aimed at protecting the flow restrictor from water blockage (Figure 3).

Bottom Line: Half of the lines collected samples into PVC yokes using a modified capillary system as commonly used in New Zealand (NZL), and half collected samples into stainless steel cylinders using a ball-bearing flow restrictor as used in Argentina (ARG), all within a 10-day time frame, either daily, across two consecutive 5-day periods or across one 10-day period (in duplicate).The NZL system had greater sampling success (97.3 vs. 79.5%) and yielded more consistent CH₄ emission estimates than the ARG system.Emission estimates from NZL daily, NZL 5-day and NZL 10-day samplings were 114, 110 and 111 g d(-1), respectively.

View Article: PubMed Central - PubMed

Affiliation: Grasslands Research Centre, AgResearch Limited, Palmerston North 4442, New Zealand. cesar.pinares@agresearch.co.nz.

ABSTRACT
The daily sample collection protocol of the sulphur hexafluoride (SF₆) tracer technique for the estimation of methane (CH₄) emissions from ruminants may not be practical under extensive grazing systems. Here, under controlled conditions, we evaluated extended periods of sampling as an alternative to daily sample collections. Eight rumen-fistulated cows were housed and fed lucerne silage to achieve common daily feed intakes of 6.4 kg dry matter per cow. Following SF₆ permeation tube dosing, eight sampling lines were fitted to the breath collection harness, so that a common gas mix was available to each line. Half of the lines collected samples into PVC yokes using a modified capillary system as commonly used in New Zealand (NZL), and half collected samples into stainless steel cylinders using a ball-bearing flow restrictor as used in Argentina (ARG), all within a 10-day time frame, either daily, across two consecutive 5-day periods or across one 10-day period (in duplicate). The NZL system had greater sampling success (97.3 vs. 79.5%) and yielded more consistent CH₄ emission estimates than the ARG system. Emission estimates from NZL daily, NZL 5-day and NZL 10-day samplings were 114, 110 and 111 g d(-1), respectively. Extended sample collection protocol may be feasible, but definitive evaluation of this alternative as well as sample collection systems is required under grazing situations before a decision on recommendation can be made.

No MeSH data available.