Limits...
Methods for Measuring and Estimating Methane Emission from Ruminants.

Storm IM, Hellwing AL, Nielsen NI, Madsen J - Animals (Basel) (2012)

Bottom Line: A thorough knowledge of the advantages and disadvantages of these methods is very important in order to plan experiments, understand and interpret experimental results, and compare them with other studies.Other methods under development such as the micrometeorological technique, combined feeder and CH₄ analyzer and proxy methods are briefly mentioned.Methods of choice for estimating enteric methane emission depend on aim, equipment, knowledge, time and money available, but interpretation of results obtained with a given method can be improved if knowledge about the disadvantages and advantages are used in the planning of experiments.

View Article: PubMed Central - PubMed

Affiliation: Department of Large Animal Sciences, University of Copenhagen, Grønnegårdsvej 2, DK-1870 Frederiksberg C, Denmark. imld@life.ku.dk.

ABSTRACT
This paper is a brief introduction to the different methods used to quantify the enteric methane emission from ruminants. A thorough knowledge of the advantages and disadvantages of these methods is very important in order to plan experiments, understand and interpret experimental results, and compare them with other studies. The aim of the paper is to describe the principles, advantages and disadvantages of different methods used to quantify the enteric methane emission from ruminants. The best-known methods: Chambers/respiration chambers, SF₆ technique and in vitro gas production technique and the newer CO₂ methods are described. Model estimations, which are used to calculate national budget and single cow enteric emission from intake and diet composition, are also discussed. Other methods under development such as the micrometeorological technique, combined feeder and CH₄ analyzer and proxy methods are briefly mentioned. Methods of choice for estimating enteric methane emission depend on aim, equipment, knowledge, time and money available, but interpretation of results obtained with a given method can be improved if knowledge about the disadvantages and advantages are used in the planning of experiments.

No MeSH data available.


Illustration of a wireless in vitro gas production module. The individual gas production module (a) measures pressure from fermentation in the jar continuously and releases gas at a certain set point above atmospheric pressure. Data is wirelessly transferred from all modules, which can be incubated in a water bath or an incubator (b).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4494326&req=5

animals-02-00160-f004: Illustration of a wireless in vitro gas production module. The individual gas production module (a) measures pressure from fermentation in the jar continuously and releases gas at a certain set point above atmospheric pressure. Data is wirelessly transferred from all modules, which can be incubated in a water bath or an incubator (b).

Mentions: The basic principle of IVGPTs is to ferment feed under controlled laboratory conditions employing natural rumen microbes. Feedstuffs, e.g., subjected to different treatments, are incubated at 39 °C with a mixture of rumen fluid, buffer and minerals for a certain time period, typically 24, 48, 72, 96 or 144 h (Figure 4). The amount of total gas produced during incubation is measured and its composition analyzed, to obtain data on the in vitro production of methane. At the same time it is possible to determine in vitro degradation of the feedstuffs, making it possible to determine whether a reduction in methane production is at the cost of total feed degradation. The output of IVGPT experiments is usually reported as amount of CH4 per gram dry matter (DM), per gram degraded DM (dDM) or per gram degraded NDF (dNDF).


Methods for Measuring and Estimating Methane Emission from Ruminants.

Storm IM, Hellwing AL, Nielsen NI, Madsen J - Animals (Basel) (2012)

Illustration of a wireless in vitro gas production module. The individual gas production module (a) measures pressure from fermentation in the jar continuously and releases gas at a certain set point above atmospheric pressure. Data is wirelessly transferred from all modules, which can be incubated in a water bath or an incubator (b).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494326&req=5

animals-02-00160-f004: Illustration of a wireless in vitro gas production module. The individual gas production module (a) measures pressure from fermentation in the jar continuously and releases gas at a certain set point above atmospheric pressure. Data is wirelessly transferred from all modules, which can be incubated in a water bath or an incubator (b).
Mentions: The basic principle of IVGPTs is to ferment feed under controlled laboratory conditions employing natural rumen microbes. Feedstuffs, e.g., subjected to different treatments, are incubated at 39 °C with a mixture of rumen fluid, buffer and minerals for a certain time period, typically 24, 48, 72, 96 or 144 h (Figure 4). The amount of total gas produced during incubation is measured and its composition analyzed, to obtain data on the in vitro production of methane. At the same time it is possible to determine in vitro degradation of the feedstuffs, making it possible to determine whether a reduction in methane production is at the cost of total feed degradation. The output of IVGPT experiments is usually reported as amount of CH4 per gram dry matter (DM), per gram degraded DM (dDM) or per gram degraded NDF (dNDF).

Bottom Line: A thorough knowledge of the advantages and disadvantages of these methods is very important in order to plan experiments, understand and interpret experimental results, and compare them with other studies.Other methods under development such as the micrometeorological technique, combined feeder and CH₄ analyzer and proxy methods are briefly mentioned.Methods of choice for estimating enteric methane emission depend on aim, equipment, knowledge, time and money available, but interpretation of results obtained with a given method can be improved if knowledge about the disadvantages and advantages are used in the planning of experiments.

View Article: PubMed Central - PubMed

Affiliation: Department of Large Animal Sciences, University of Copenhagen, Grønnegårdsvej 2, DK-1870 Frederiksberg C, Denmark. imld@life.ku.dk.

ABSTRACT
This paper is a brief introduction to the different methods used to quantify the enteric methane emission from ruminants. A thorough knowledge of the advantages and disadvantages of these methods is very important in order to plan experiments, understand and interpret experimental results, and compare them with other studies. The aim of the paper is to describe the principles, advantages and disadvantages of different methods used to quantify the enteric methane emission from ruminants. The best-known methods: Chambers/respiration chambers, SF₆ technique and in vitro gas production technique and the newer CO₂ methods are described. Model estimations, which are used to calculate national budget and single cow enteric emission from intake and diet composition, are also discussed. Other methods under development such as the micrometeorological technique, combined feeder and CH₄ analyzer and proxy methods are briefly mentioned. Methods of choice for estimating enteric methane emission depend on aim, equipment, knowledge, time and money available, but interpretation of results obtained with a given method can be improved if knowledge about the disadvantages and advantages are used in the planning of experiments.

No MeSH data available.