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Methods for Measuring and Estimating Methane Emission from Ruminants.

Storm IM, Hellwing AL, Nielsen NI, Madsen J - Animals (Basel) (2012)

Bottom Line: A thorough knowledge of the advantages and disadvantages of these methods is very important in order to plan experiments, understand and interpret experimental results, and compare them with other studies.Other methods under development such as the micrometeorological technique, combined feeder and CH₄ analyzer and proxy methods are briefly mentioned.Methods of choice for estimating enteric methane emission depend on aim, equipment, knowledge, time and money available, but interpretation of results obtained with a given method can be improved if knowledge about the disadvantages and advantages are used in the planning of experiments.

View Article: PubMed Central - PubMed

Affiliation: Department of Large Animal Sciences, University of Copenhagen, Grønnegårdsvej 2, DK-1870 Frederiksberg C, Denmark. imld@life.ku.dk.

ABSTRACT
This paper is a brief introduction to the different methods used to quantify the enteric methane emission from ruminants. A thorough knowledge of the advantages and disadvantages of these methods is very important in order to plan experiments, understand and interpret experimental results, and compare them with other studies. The aim of the paper is to describe the principles, advantages and disadvantages of different methods used to quantify the enteric methane emission from ruminants. The best-known methods: Chambers/respiration chambers, SF₆ technique and in vitro gas production technique and the newer CO₂ methods are described. Model estimations, which are used to calculate national budget and single cow enteric emission from intake and diet composition, are also discussed. Other methods under development such as the micrometeorological technique, combined feeder and CH₄ analyzer and proxy methods are briefly mentioned. Methods of choice for estimating enteric methane emission depend on aim, equipment, knowledge, time and money available, but interpretation of results obtained with a given method can be improved if knowledge about the disadvantages and advantages are used in the planning of experiments.

No MeSH data available.


Illustration of the SF6 tracer technique. Reprinted with permission from [22]. Copyright (1994) American Chemical Society.
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animals-02-00160-f003: Illustration of the SF6 tracer technique. Reprinted with permission from [22]. Copyright (1994) American Chemical Society.

Mentions: SF6 is filled into small permeation tubes. The rate of diffusion of SF6 out of the permeation tubes is measured by placing them in a 39 °C water bath and measuring the daily weight loss until it is stable. The permeation tube is then placed in the rumen of an experimental animal and collection of air can start. The sampling apparatus consists of a collection canister, a halter and capillary tubing. The capillary tubing is placed at the nose of the animal and connected with the evacuated canister (Figure 3). The tubing regulates the sampling rate. The sampling time is typically one day [22,29,41], but emission estimates from shorter time intervals have been published [30,36]. The concentration of SF6 and CH4 in the canister is determined by gas chromatography. For more detailed description of equipment and guidance see [41]. The methane emission is calculated from the release rate of SF6 and concentration of SF6 and CH4 in the containers in excess of background level [31] as described in Equation (1).(1)FCH4=Ftracer·CCH4measured−CCH4atmCtracermeasured−Ctraceratmwhere is the total production of CH4, Ftracer is the total production or release of SF6, and are the measured concentrations of CH4 and SF6 in the experimental entity e.g., in the unit of ppm, while and are the concentrations of CH4 and SF6 in atmospheric or background air, measured with the same analyzer and in the same unit.


Methods for Measuring and Estimating Methane Emission from Ruminants.

Storm IM, Hellwing AL, Nielsen NI, Madsen J - Animals (Basel) (2012)

Illustration of the SF6 tracer technique. Reprinted with permission from [22]. Copyright (1994) American Chemical Society.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494326&req=5

animals-02-00160-f003: Illustration of the SF6 tracer technique. Reprinted with permission from [22]. Copyright (1994) American Chemical Society.
Mentions: SF6 is filled into small permeation tubes. The rate of diffusion of SF6 out of the permeation tubes is measured by placing them in a 39 °C water bath and measuring the daily weight loss until it is stable. The permeation tube is then placed in the rumen of an experimental animal and collection of air can start. The sampling apparatus consists of a collection canister, a halter and capillary tubing. The capillary tubing is placed at the nose of the animal and connected with the evacuated canister (Figure 3). The tubing regulates the sampling rate. The sampling time is typically one day [22,29,41], but emission estimates from shorter time intervals have been published [30,36]. The concentration of SF6 and CH4 in the canister is determined by gas chromatography. For more detailed description of equipment and guidance see [41]. The methane emission is calculated from the release rate of SF6 and concentration of SF6 and CH4 in the containers in excess of background level [31] as described in Equation (1).(1)FCH4=Ftracer·CCH4measured−CCH4atmCtracermeasured−Ctraceratmwhere is the total production of CH4, Ftracer is the total production or release of SF6, and are the measured concentrations of CH4 and SF6 in the experimental entity e.g., in the unit of ppm, while and are the concentrations of CH4 and SF6 in atmospheric or background air, measured with the same analyzer and in the same unit.

Bottom Line: A thorough knowledge of the advantages and disadvantages of these methods is very important in order to plan experiments, understand and interpret experimental results, and compare them with other studies.Other methods under development such as the micrometeorological technique, combined feeder and CH₄ analyzer and proxy methods are briefly mentioned.Methods of choice for estimating enteric methane emission depend on aim, equipment, knowledge, time and money available, but interpretation of results obtained with a given method can be improved if knowledge about the disadvantages and advantages are used in the planning of experiments.

View Article: PubMed Central - PubMed

Affiliation: Department of Large Animal Sciences, University of Copenhagen, Grønnegårdsvej 2, DK-1870 Frederiksberg C, Denmark. imld@life.ku.dk.

ABSTRACT
This paper is a brief introduction to the different methods used to quantify the enteric methane emission from ruminants. A thorough knowledge of the advantages and disadvantages of these methods is very important in order to plan experiments, understand and interpret experimental results, and compare them with other studies. The aim of the paper is to describe the principles, advantages and disadvantages of different methods used to quantify the enteric methane emission from ruminants. The best-known methods: Chambers/respiration chambers, SF₆ technique and in vitro gas production technique and the newer CO₂ methods are described. Model estimations, which are used to calculate national budget and single cow enteric emission from intake and diet composition, are also discussed. Other methods under development such as the micrometeorological technique, combined feeder and CH₄ analyzer and proxy methods are briefly mentioned. Methods of choice for estimating enteric methane emission depend on aim, equipment, knowledge, time and money available, but interpretation of results obtained with a given method can be improved if knowledge about the disadvantages and advantages are used in the planning of experiments.

No MeSH data available.