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Overview of Serological Techniques for Influenza Vaccine Evaluation: Past, Present and Future.

Trombetta CM, Perini D, Mather S, Temperton N, Montomoli E - Vaccines (Basel) (2014)

Bottom Line: HI and SRH are established and reproducible techniques, whereas VN is more demanding.Every new influenza vaccine needs to fulfil the strict criteria issued by the European Medicines Agency (EMA) in order to be licensed.These criteria currently apply exclusively to SRH and HI assays and refer to two different target groups-healthy adults and the elderly, but other vaccine recipient age groups have not been considered (i.e., children).

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Developmental Medicine, University of Siena, Via Aldo Moro, 53100 Siena, Italy. trombetta@unisi.it.

ABSTRACT
Serological techniques commonly used to quantify influenza-specific antibodies include the Haemagglutination Inhibition (HI), Single Radial Haemolysis (SRH) and Virus Neutralization (VN) assays. HI and SRH are established and reproducible techniques, whereas VN is more demanding. Every new influenza vaccine needs to fulfil the strict criteria issued by the European Medicines Agency (EMA) in order to be licensed. These criteria currently apply exclusively to SRH and HI assays and refer to two different target groups-healthy adults and the elderly, but other vaccine recipient age groups have not been considered (i.e., children). The purpose of this timely review is to highlight the current scenario on correlates of protection concerning influenza vaccines and underline the need to revise the criteria and assays currently in use. In addition to SRH and HI assays, the technical advantages provided by other techniques such as the VN assay, pseudotype-based neutralization assay, neuraminidase and cell-mediated immunity assays need to be considered and regulated via EMA criteria, considering the many significant advantages that they could offer for the development of effective vaccines.

No MeSH data available.


Related in: MedlinePlus

Antibody titres measured by HI, VN and SRH assays after a vaccination with a non-adjuvanted vaccine or an MF59-adjuvanted influenza A/Duck/Singapore/97 (H5N3) vaccine. At each visit, the HI assay was shown to underestimate antibody responses when compared to VN and SRH assays [106].
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vaccines-02-00707-f004: Antibody titres measured by HI, VN and SRH assays after a vaccination with a non-adjuvanted vaccine or an MF59-adjuvanted influenza A/Duck/Singapore/97 (H5N3) vaccine. At each visit, the HI assay was shown to underestimate antibody responses when compared to VN and SRH assays [106].

Mentions: Following the emergence of avian influenza viruses capable of causing infection in humans, and limited use of HI assays for these viruses due to an underestimation of the human immune response raised against these pathogens [104,105,106] (Figure 4), SRH has been widely used as a sensitive and specific technique for the detection of human antibodies directed against avian influenza viruses in clinical trials [107,108,109].


Overview of Serological Techniques for Influenza Vaccine Evaluation: Past, Present and Future.

Trombetta CM, Perini D, Mather S, Temperton N, Montomoli E - Vaccines (Basel) (2014)

Antibody titres measured by HI, VN and SRH assays after a vaccination with a non-adjuvanted vaccine or an MF59-adjuvanted influenza A/Duck/Singapore/97 (H5N3) vaccine. At each visit, the HI assay was shown to underestimate antibody responses when compared to VN and SRH assays [106].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4494249&req=5

vaccines-02-00707-f004: Antibody titres measured by HI, VN and SRH assays after a vaccination with a non-adjuvanted vaccine or an MF59-adjuvanted influenza A/Duck/Singapore/97 (H5N3) vaccine. At each visit, the HI assay was shown to underestimate antibody responses when compared to VN and SRH assays [106].
Mentions: Following the emergence of avian influenza viruses capable of causing infection in humans, and limited use of HI assays for these viruses due to an underestimation of the human immune response raised against these pathogens [104,105,106] (Figure 4), SRH has been widely used as a sensitive and specific technique for the detection of human antibodies directed against avian influenza viruses in clinical trials [107,108,109].

Bottom Line: HI and SRH are established and reproducible techniques, whereas VN is more demanding.Every new influenza vaccine needs to fulfil the strict criteria issued by the European Medicines Agency (EMA) in order to be licensed.These criteria currently apply exclusively to SRH and HI assays and refer to two different target groups-healthy adults and the elderly, but other vaccine recipient age groups have not been considered (i.e., children).

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Developmental Medicine, University of Siena, Via Aldo Moro, 53100 Siena, Italy. trombetta@unisi.it.

ABSTRACT
Serological techniques commonly used to quantify influenza-specific antibodies include the Haemagglutination Inhibition (HI), Single Radial Haemolysis (SRH) and Virus Neutralization (VN) assays. HI and SRH are established and reproducible techniques, whereas VN is more demanding. Every new influenza vaccine needs to fulfil the strict criteria issued by the European Medicines Agency (EMA) in order to be licensed. These criteria currently apply exclusively to SRH and HI assays and refer to two different target groups-healthy adults and the elderly, but other vaccine recipient age groups have not been considered (i.e., children). The purpose of this timely review is to highlight the current scenario on correlates of protection concerning influenza vaccines and underline the need to revise the criteria and assays currently in use. In addition to SRH and HI assays, the technical advantages provided by other techniques such as the VN assay, pseudotype-based neutralization assay, neuraminidase and cell-mediated immunity assays need to be considered and regulated via EMA criteria, considering the many significant advantages that they could offer for the development of effective vaccines.

No MeSH data available.


Related in: MedlinePlus